A Duplex Real-Time qPCR Assay for the Quantification of Human Nuclear and Mitochondrial DNA in Forensic Samples: Implications for Quantifying DNA in Degraded Samples

2005 ◽  
Vol 50 (5) ◽  
pp. 1-17 ◽  
Author(s):  
Mark D. Timken ◽  
Katie L. Swango ◽  
Cristián Orrego ◽  
Martin R. Buoncristiani
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Qpcr Assay ◽  
Degraded Samples ◽  
Forensic Samples ◽  
Real Time Qpcr

scholarly journals Real-time qPCR Assay for the TYLCV Titer in Relation to Symptoms-Based Disease Severity Scales

2017 ◽  
Vol 19 (01) ◽  
pp. 145-151 ◽  
Author(s):  
Um e Ammara ◽  
Abdullah Mohammed Al-Sadi ◽  
Adel Al-Shihi ◽  
Imran Amin
Keyword(s):  
Real Time ◽  
Disease Severity ◽  
Qpcr Assay ◽  
Real Time Qpcr

scholarly journals Detection of Increased Amounts of Cell-Free Fetal DNA with Short PCR Amplicons

2010 ◽  
Vol 56 (1) ◽  
pp. 136-138 ◽  
Author(s):  
Aleksandra Sikora ◽  
Bernhard G Zimmermann ◽  
Corinne Rusterholz ◽  
Daniella Birri ◽  
Varaprasad Kolla ◽  
...  
Keyword(s):  
Real Time ◽  
Digital Pcr ◽  
Maternal Plasma ◽  
Qpcr Assay ◽  
Detection Capability ◽  
Real Time Quantitative Pcr ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Amplicon Size ◽  
Real Time Qpcr

Abstract Aim: A digital PCR approach has recently been suggested to detect greater amounts of cell-free fetal DNA in maternal plasma than conventional real-time quantitative PCR (qPCR). Because the digital qPCR approach uses shorter PCR amplicons than the real-time qPCR assay, we investigated whether a real-time qPCR assay appropriately modified for such short amplicons would improve the detection of cell-free fetal DNA. Method: We developed a novel universal-template (UT) real-time qPCR assay that was specific for the DYS14 sequence on Y chromosome and had a short amplicon size of 50 bp. We examined this “short” assay with 50 maternal plasma samples and compared the results with those for a conventional real-time qPCR assay of the same locus but with a longer amplicon (84 bp). Results: Qualitatively, both assays detected male cell-free fetal DNA with the same specificity and detection capability. Quantitatively, however, the new UT real-time qPCR assay for shorter amplicons detected, on average, almost 1.6-fold more cell-free fetal DNA than the conventional real-time qPCR assay with longer amplicons. Conclusions: The use of short PCR amplicons improves the detection of cell-free fetal DNA. This feature may prove useful in attempts to detect cell-free fetal DNA under conditions in which the amount of template is low, such as in samples obtained early in pregnancy.

Development of fast and sensitive real-time qPCR assay based on a novel probe for detection of porcine DNA in food sample

LWT ◽  
2017 ◽  
Vol 84 ◽  
pp. 686-692 ◽  
Author(s):  
Hamadah Lubis ◽  
Nur Thaqifah Salihah ◽  
Mohammad Mosharraf Hossain ◽  
Minhaz Uddin Ahmed
Keyword(s):  
Real Time ◽  
Food Sample ◽  
Qpcr Assay ◽  
Real Time Qpcr
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