Development of a multiplex real-time qPCR assay for simultaneous enumeration of up to four marine toxic bloom-forming microalgal species

Harmful Algae ◽  
2015 ◽  
Vol 48 ◽  
pp. 37-43 ◽  
Author(s):  
Lisa K. Eckford-Soper ◽  
Niels Daugbjerg
Keyword(s):  
Real Time ◽  
Qpcr Assay ◽  
Toxic Bloom ◽  
Microalgal Species ◽  
Real Time Qpcr

scholarly journals Real-time qPCR Assay for the TYLCV Titer in Relation to Symptoms-Based Disease Severity Scales

2017 ◽  
Vol 19 (01) ◽  
pp. 145-151 ◽  
Author(s):  
Um e Ammara ◽  
Abdullah Mohammed Al-Sadi ◽  
Adel Al-Shihi ◽  
Imran Amin
Keyword(s):  
Real Time ◽  
Disease Severity ◽  
Qpcr Assay ◽  
Real Time Qpcr

scholarly journals Detection of Increased Amounts of Cell-Free Fetal DNA with Short PCR Amplicons

2010 ◽  
Vol 56 (1) ◽  
pp. 136-138 ◽  
Author(s):  
Aleksandra Sikora ◽  
Bernhard G Zimmermann ◽  
Corinne Rusterholz ◽  
Daniella Birri ◽  
Varaprasad Kolla ◽  
...  
Keyword(s):  
Real Time ◽  
Digital Pcr ◽  
Maternal Plasma ◽  
Qpcr Assay ◽  
Detection Capability ◽  
Real Time Quantitative Pcr ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Amplicon Size ◽  
Real Time Qpcr

Abstract Aim: A digital PCR approach has recently been suggested to detect greater amounts of cell-free fetal DNA in maternal plasma than conventional real-time quantitative PCR (qPCR). Because the digital qPCR approach uses shorter PCR amplicons than the real-time qPCR assay, we investigated whether a real-time qPCR assay appropriately modified for such short amplicons would improve the detection of cell-free fetal DNA. Method: We developed a novel universal-template (UT) real-time qPCR assay that was specific for the DYS14 sequence on Y chromosome and had a short amplicon size of 50 bp. We examined this “short” assay with 50 maternal plasma samples and compared the results with those for a conventional real-time qPCR assay of the same locus but with a longer amplicon (84 bp). Results: Qualitatively, both assays detected male cell-free fetal DNA with the same specificity and detection capability. Quantitatively, however, the new UT real-time qPCR assay for shorter amplicons detected, on average, almost 1.6-fold more cell-free fetal DNA than the conventional real-time qPCR assay with longer amplicons. Conclusions: The use of short PCR amplicons improves the detection of cell-free fetal DNA. This feature may prove useful in attempts to detect cell-free fetal DNA under conditions in which the amount of template is low, such as in samples obtained early in pregnancy.

Development of fast and sensitive real-time qPCR assay based on a novel probe for detection of porcine DNA in food sample

LWT ◽  
2017 ◽  
Vol 84 ◽  
pp. 686-692 ◽  
Author(s):  
Hamadah Lubis ◽  
Nur Thaqifah Salihah ◽  
Mohammad Mosharraf Hossain ◽  
Minhaz Uddin Ahmed
Keyword(s):  
Real Time ◽  
Food Sample ◽  
Qpcr Assay ◽  
Real Time Qpcr

scholarly journals Detection of Viable Mycobacterium ulcerans in Clinical Samples by a Novel Combined 16S rRNA Reverse Transcriptase/IS2404 Real-Time qPCR Assay

2012 ◽  
Vol 6 (8) ◽  
pp. e1756 ◽  
Author(s):  
Marcus Beissner ◽  
Dominik Symank ◽  
Richard Odame Phillips ◽  
Yaw Ampem Amoako ◽  
Nana-Yaa Awua-Boateng ◽  
...  
Keyword(s):  
16S Rrna ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Mycobacterium Ulcerans ◽  
Qpcr Assay ◽  
Clinical Samples ◽  
Real Time Qpcr
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