Evaluation of Iodine-Benzoflavone and Ruthenium Tetroxide Spray Reagents for the Detection of Latent Fingermarks at the Crime Scene

Katherine Flynn; Philip Maynard; Eric Du Pasquier; Chris Lennard; Milutin Stoilovic; Claude Roux

doi:10.1520/jfs2003197

Evaluation of Iodine-Benzoflavone and Ruthenium Tetroxide Spray Reagents for the Detection of Latent Fingermarks at the Crime Scene

Journal of Forensic Sciences ◽

10.1520/jfs2003197 ◽

2004 ◽

Vol 49 (4) ◽

pp. 1-9 ◽

Cited By ~ 21

Author(s):

Katherine Flynn ◽

Philip Maynard ◽

Eric Du Pasquier ◽

Chris Lennard ◽

Milutin Stoilovic ◽

...

Keyword(s):

Crime Scene ◽

Ruthenium Tetroxide ◽

Spray Reagents

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Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169080 ◽

1994 ◽

Vol 52 ◽

pp. 270-271

Author(s):

Henry H. Eichelberger ◽

John G. Baust ◽

Robert G. Van Buskirk

Keyword(s):

Cold Storage ◽

Structural Integrity ◽

Culture Media ◽

Cell Structure ◽

Natural State ◽

Sodium Cacodylate ◽

Ruthenium Tetroxide ◽

Cacodylate Buffer ◽

Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

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