Sex Identification of Elk (Cervus elaphus canadensis), Moose (Alces alces), and White-Tailed Deer (Odocoileus virginianus) Using the Polymerase Chain Reaction

1998 ◽  
Vol 43 (3) ◽  
pp. 16172J ◽  
Author(s):  
P. J. Wilson ◽  
B. N. White
Keyword(s):  
Polymerase Chain Reaction ◽  
Odocoileus Virginianus ◽  
Cervus Elaphus ◽  
Alces Alces ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction-Based Sex Identification in the Greater Roadrunner

2010 ◽  
Vol 74 (6) ◽  
pp. 1395-1399
Author(s):  
CARLOS A. SANTAMARIA ◽  
SAMUEL KELLEY ◽  
GERRAL G. SCHULZ ◽  
DEAN RANSOM ◽  
LUIS A. HURTADO
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain

Sex identification of pigeons using polymerase chain reaction analysis with simple DNA extraction

2019 ◽  
Vol 12 (2) ◽  
pp. 45-48
Author(s):  
Shao-jie Liang ◽  
Ming-xia Chen ◽  
Chun-qi Gao ◽  
Hui-chao Yan ◽  
Guo-long Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Polymerase Chain Reaction Analysis ◽  
Sex Identification ◽  
Z Chromosome ◽  
W Chromosome ◽  
Chain Reaction ◽  
Assay Time ◽  
Polymerase Chain ◽  
The Cost

Sex identification plays an important role in avian production. Hitherto, it is difficult to distinguish the sexes of monomorphic birds based on their external features. The chromo-helicase-DNA-binding genes contain CHD-W gene and CHD-Z gene, which are located on the W chromosome and Z chromosome, respectively. Since CHD-W gene is unique to females, the polymerase chain reaction can be used for sex identification. However, extracting DNA procedures for verifying the sex is tedious and expensive. To address these disadvantages, the objective of this study was to develop a simple DNA extraction assay to efficiently process blood, liver, and feather samples. The results showed that 2% dimethylsulfoxide was suitable for processing blood, and phosphate-buffered saline was suitable for processing liver and feather samples. The specific primers were designed, and the length of the targets is 474 bp on Z chromosome and 319 bp on W chromosome. The pigeons were identified as females based on the presence of two bands on the gel, and as males based on the presence of one band. Taken together, our results suggested that feather samples were more appropriate than blood or liver for sex identification of pigeons. Compared to the traditional DNA extraction, this method shortened the assay time and reduced the cost.

scholarly journals Sex identification by polymerase chain reaction using a Y-autosome homologous primer set

1993 ◽  
Vol 38 (4) ◽  
pp. 429-431
Author(s):  
Taku Iida ◽  
Yutaka Nakahori ◽  
Karo Tanaka ◽  
Osamu Tsutsumi ◽  
Yuji Taketani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)

2009 ◽  
Vol 46 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Andrzej Dybus ◽  
Anna Siemierz ◽  
Dariusz Wysocki ◽  
Iwona Szatkowska ◽  
Magdalena Muszyńska ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Bird Species ◽  
Sex Identification ◽  
Chain Reaction ◽  
Turdus Merula ◽  
Polymerase Chain

Evaluation of the applicability of polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds (Turdus merula)Turdus merulais one of most common bird species in Europe. This study verified a method for its sex identification by PCR with P2/P8 primers, based on theCHD1gene polymorphism, proposed in earlier studies as a universal tool for sex identification in most bird species. Although there are some reports that PCR cannot determine sex in Eurasian blackbirds due to a lack of differences in intron lengths ofCHD1-ZandCHD1-Wgenes, our study showed that such a possibility does exist, so it can contribute to an increased understanding of the biology of this species.

Sex identification of forensic specimens by polymerase chain reaction (PCR): Two alternative methods

1991 ◽  
Vol 49 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Atsushi Akane ◽  
Hiroshi Shiono ◽  
Kazuo Matsubara ◽  
Yutaka Nakahori ◽  
Satoko Seki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Identification ◽  
Alternative Methods ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Retrospective study of central nervous system lesions and association with Parelaphostrongylus species by histology and specific nested polymerase chain reaction in domestic camelids and wild ungulates

2014 ◽  
Vol 26 (6) ◽  
pp. 748-754 ◽  
Author(s):  
Carrie L. Dobey ◽  
Caroline Grunenwald ◽  
Shelley J. Newman ◽  
Lisa Muller ◽  
Richard W. Gerhold
Keyword(s):  
Polymerase Chain Reaction ◽  
Cervus Elaphus ◽  
Plasma Cells ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Molecular Tests ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Polymerase Chain ◽  
Formalin Fixed

Formalin-fixed, paraffin-embedded tissues from elk ( Cervus elaphus), goats, and camelids with case histories and lesions suggestive of Parelaphostrongylus tenuis were examined by histology to characterize lesions that could aid in definitively diagnosing P. tenuis infection. Additionally, sections of paraffin-embedded tissue were used in a nested polymerase chain reaction (nPCR) using Parelaphostrongylus-specific primers to determine how PCR results corresponded with histological findings. Histological changes in brain and spinal cord consisted of linear tracks of hemorrhage; tracks or perivascular accumulations of hemosiderin-laden macrophages; acute foci of axonal degeneration and/or linear glial scars; and perivascular, parenchymal, or meningeal accumulations of eosinophils and/or lymphocytes and plasma cells. Of the 43 samples with histologic lesions consistent with neural larval migrans, 19 were PCR positive; however, only 8 were confirmed Parelaphostrongylus by DNA sequencing. Additionally, 1 goat was identified with a protostrongylid that had a 97% identity to both Parelaphostrongylus odocoilei and a protostrongylid nematode from pampas deer ( Ozotoceros bezoarticus celer) from Argentina. None of the histologic lesions individually or in combination correlated statistically to positive molecular tests for the nematode. The results indicate that it is possible to extract Parelaphostrongylus DNA from formalin-fixed, paraffin-embedded tissue, but extended fixation presumably can cause DNA crosslinking. Nested PCR provides another diagnostic tool to identify the cause of neurologic disease in camelids and elk with histologic lesions consistent with neural larval migrans. Furthermore, potential novel protostrongylid DNA was detected from a goat with lesions consistent with P. tenuis infection, suggesting that other neurotropic Parelaphostrongylus species may occur locally.

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