Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia

10.1520/e1295 ◽  
2008 ◽  
Author(s):  
Keyword(s):  
Ceriodaphnia Dubia ◽  
Toxicity Tests

Modeling the Bioavailability of Nickel and Zinc to Ceriodaphnia dubia and Neocloeon triangulifer in Toxicity Tests with Natural Waters

2021 ◽  
Author(s):  
John M Besser ◽  
Chris D Ivey ◽  
Jeffery A Steevens ◽  
Danielle Cleveland ◽  
David Soucek ◽  
...  
Keyword(s):  
Natural Waters ◽  
Ceriodaphnia Dubia ◽  
Toxicity Tests

Bicarbonate as a Potential Confounding Factor in Cladoceran Toxicity Assessments of Pore Water from Contaminated Sediments

1992 ◽  
Vol 49 (8) ◽  
pp. 1633-1640 ◽  
Author(s):  
Robert A. Hoke ◽  
William R. Gala ◽  
James B. Drake ◽  
John P. Giesy ◽  
Stan Flegler
Keyword(s):  
Pore Water ◽  
Ceriodaphnia Dubia ◽  
Toxicity Tests ◽  
Metabolic Inhibitor ◽  
Pore Waters ◽  
Sediment Pore Water ◽  
International Joint Commission ◽  
Reference Toxicant ◽  
Area Of Concern ◽  
Pore Water Sample

Elevated alkalinity values measured in sediment pore water samples from the Grand Calumet River–Indiana Harbor Canal, an International joint Commission Area of Concern (AOC), caused concern over the potential effects of alkalinity on cladoceran test responses. Forty-eight-hour tests with NaHCO3 and NaCl as reference toxicants indicated that NaHCO3 toxicity to Daphnia magna and Ceriodaphnia dubia was due to both Na+ and HCO3−, although greater HCO3− toxicity was observed to D. magna. Theoretical HCO3− concentrations in sediment pore waters from the AOC demonstrated sufficient HCO3− in several samples to produce acute toxicity, based on 48-h LC50s from the reference toxicant tests. X-ray microanalysis was conducted to qualitatively assess the effects on internal Cl− levels of D. magna after exposure to NaHCO3, to an AOC sediment pore water sample, and to NaSCN, a metabolic inhibitor of Cl− uptake. Based on these results the proposed mechanism of HCO3− toxicity to D. magna is the inhibition of the active uptake of Cl− from water. We suggest that pore water alkalinity should be considered when interpreting the results of sediment pore water and effluent toxicity tests with D. magna, other cladocerans, and, perhaps, other invertebrates and fish.

Daphnia magna and Ceriodaphnia dubia Have Similar Sensitivity in Standard Acute and Chronic Toxicity Tests

2021 ◽  
Author(s):  
Kristin A. Connors ◽  
Jessica L. Brill ◽  
Teresa Norberg‐King ◽  
Mace G. Barron ◽  
Greg Carr ◽  
...  
Keyword(s):  
Daphnia Magna ◽  
Chronic Toxicity ◽  
Ceriodaphnia Dubia ◽  
Toxicity Tests ◽  
Acute And Chronic Toxicity

scholarly journals The influence of algal densities on the toxicity of chromium for Ceriodaphnia dubia Richard (Cladocera, Crustacea)

2008 ◽  
Vol 68 (2) ◽  
pp. 341-348 ◽  
Author(s):  
S. Rodgher ◽  
ELG. Espíndola
Keyword(s):  
Metal Toxicity ◽  
Aquatic Organisms ◽  
Ceriodaphnia Dubia ◽  
Chromium Concentration ◽  
Pseudokirchneriella Subcapitata ◽  
Toxicity Tests ◽  
Algal Density ◽  
Test Organisms ◽  
Survival And Reproduction ◽  
Ceriodaphnia Dubia Richard

Food availability may affect metal toxicity for aquatic organisms. In the present study, the influence of high, medium and low densities of the algae Pseudokirchneriella subcapitata (10(6), 10(5) and 10(4) cells.mL-1, respectively) on the chronic toxicity of chromium to the cladoceran Ceriodaphnia dubia was investigated. C. dubia was exposed to a range of chromium concentration from 2.71 to 34.04 µg.L-1 and fed with algae at various densities. In another experiment, the green alga was exposed to chromium concentrations (94 to 774 µg.L-1) and supplied as food in different densities to zooplankton. The survival and reproduction of the cladoceran were measured in these toxicity tests. The IC50 for Cr to P. subcapitata and metal accumulated by algal cells were determined. The results of a bifactorial analysis (metal versus algal densities) showed that metal toxicity to zooplankton was dependent on algal densities. Significant toxic effects on the reproduction and survival of C. dubia were observed at 8.73, 18.22 and 34.04 µg.L-1 Cr when the test organisms were fed with 10(6) cells.mL-1 of P. subcapitata. Although the chlorophyta retain low chromium content, a decrease in the reproduction and survival of C. dubia occurred when they were fed with high algal density contaminated with 774 µg.L-1 Cr. It was concluded that high algal density have an appreciable influence on chromium toxicity to daphnids.

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