scholarly journals Protective Effects of Pyruvic Acid Salt Against Lithium Toxicity and Oxidative Damage in Human Blood Mononuclear Cells

2019 ◽  
Vol 9 (2) ◽  
pp. 302-306 ◽  
Author(s):  
Evgenii Plotnikov ◽  
Innokenty Losenkov ◽  
Elena Epimakhova ◽  
Nikolay Bohan
Keyword(s):  
Oxidative Stress ◽  
Human Blood ◽  
Mononuclear Cells ◽  
Cell Metabolism ◽  
Mood Stabilizer ◽  
Positive Influence ◽  
Protective Effects ◽  
Healthy Donors ◽  
Potential Benefits

Purpose: Aim of present work was to study cytoprotective properties of lithium pyruvate, as a prospective pharmacological agent. Pyruvate has a lot of potential benefits due to positive influence on cell metabolism. Lithium is "gold-standard" mood-stabilizer. Combination of both may lead advantages. Methods: Lithium pyruvate was tested as cytoprotector on human blood mononuclears under induced oxidative stress. Cells were obtained from healthy donors and patients with alcoholism. The detection of cell viability, apoptosis and determination of oxidative stress level were conducted by flow cytometry. Results: Lithium pyruvate showed excellent cytoprotective properties in normal and oxidation conditions. This effect was independent from cell donor health status. It was shown on cells from healthy donors and alcoholics patients. Conclusion: Obtained results allow considering lithium pyruvate as potential normothymic agents (mood stabilizer) with excellent cytoprotective properties.

scholarly journals Role of protein kinases JNK and p38 in regulation of mononuclear leucocytes apoptosis in oxidative stress

2008 ◽  
Vol 7 (3) ◽  
pp. 38-43 ◽  
Author(s):  
N. Yu. Chasovskikh
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Transmembrane Potential ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Stress Induction ◽  
Healthy Donors ◽  
Mononuclear Leucocytes

Programmed cell death of peripheral blood mononuclear leucocytes taken from healthy donors and cultivated with various concentration of Н2О2, selective inhibitors of JNK (SP600125), 38 (ML3403) and in case of pneumonia was investigated. Intensification of intracellular production of reactive oxy р МАРК - gen species was accompanied by the increase in number of apoptotic and TNFR1-presented cells and mononuclears with reduced value of mitochondrial transmembrane potential in a case of oxidative stress induction with 1 mM hydrogen peroxide and in blood taken from patients with pneumonia. Action of inhibitors SP600125 and ML3403 in vitro in oxidative stress conditions prevents the increase in number of annexin- positive mononuclear cells, that confirms the participation of JNK and 38 -kinases in mechanisms of oxidative stress-mediated apoptosis dysregulation.

scholarly journals Mitochondria Turnover and Lysosomal Function in Hematopoietic Stem Cell Metabolism

2021 ◽  
Vol 22 (9) ◽  
pp. 4627
Author(s):  
Makiko Mochizuki-Kashio ◽  
Hiroko Shiozaki ◽  
Toshio Suda ◽  
Ayako Nakamura-Ishizu
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Cell Fate ◽  
Metabolic Regulation ◽  
Anaerobic Metabolism ◽  
Cell Metabolism ◽  
Hematopoietic Stem ◽  
Lysosomal Function ◽  
Stem Cell Metabolism

Hematopoietic stem cells (HSCs) reside in a hypoxic microenvironment that enables glycolysis-fueled metabolism and reduces oxidative stress. Nonetheless, metabolic regulation in organelles such as the mitochondria and lysosomes as well as autophagic processes have been implicated as essential for the determination of HSC cell fate. This review encompasses the current understanding of anaerobic metabolism in HSCs as well as the emerging roles of mitochondrial metabolism and lysosomal regulation for hematopoietic homeostasis.

scholarly journals Growth on Alpha-Ketoglutarate Increases Oxidative Stress Resistance in the YeastSaccharomyces cerevisiae

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Maria Bayliak ◽  
Nadia Burdyliuk ◽  
Volodymyr Lushchak
Keyword(s):  
Oxidative Stress ◽  
Stress Resistance ◽  
Cell Metabolism ◽  
Transition Metal Ions ◽  
Activity Level ◽  
Yeast Cells ◽  
Protective Effects ◽  
Wild Type ◽  
Genes Encoding ◽  
Important Intermediate

Alpha-ketoglutarate (AKG) is an important intermediate in cell metabolism, linking anabolic and catabolic processes. The effect of exogenous AKG on stress resistance inS. cerevisiaecells was studied. The growth on AKG increased resistance of yeast cells to stresses, but the effects depended on AKG concentration and type of stressor. Wild-type yeast cells grown on AKG were more resistant to hydrogen peroxide, menadione, and transition metal ions (Fe2+and Cu2+) but not to ethanol and heat stress as compared with control ones. Deficiency in SODs or catalases abolished stress-protective effects of AKG. AKG-supplemented growth led to higher values of total metabolic activity, level of low-molecular mass thiols, and activities of catalase and glutathione reductase in wild-type cells compared with the control. The results suggest that exogenous AKG may enhance cell metabolism leading to induction of mild oxidative stress. It turn, it results in activation of antioxidant system that increases resistance ofS. cerevisiaecells to H2O2and other stresses. The presence of genes encoding SODs or catalases is required for the expression of protective effects of AKG.

scholarly journals Effect of Sanguisorba Minor on Scopolamine-induced Memory Loss in Rat: Involvement of Oxidative Stress and Acetylcholinesterase

2021 ◽  
Author(s):  
Zeinab Hosseini ◽  
Fatemeh Mansouritorghabeh ◽  
Faezeh Sadat Hosseini Kakhki ◽  
Mahmoud Hosseini ◽  
Hassan Rakhshandeh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ache Activity ◽  
Antioxidant Activities ◽  
Memory Loss ◽  
Ethanolic Extract ◽  
Protective Effects ◽  
Oxidative Stress Biomarkers ◽  
Potential Benefits ◽  
Sanguisorba Minor ◽  
The Brain

Abstract Sanguisorba minor (S. minor) has neuroprotective and antioxidant activities; nevertheless, its potential benefits in ameliorating learning and memory functions have not been explored yet. So, in this study, rats were treated with S. minor hydro-ethanolic extract (50, 100, and 200 mg/kg, intraperitoneal (i.p.)) as well as rivastigmine (0.5 mg/kg, i.p.) for 21 consecutive days. Thereafter, their behavioral performance was assessed using Morris water maze (MWM) and passive avoidance (PA) tasks. Scopolamine was also injected 30 min before conducting the tasks. Finally, oxidative stress biomarkers and acetyl cholinesterase (AChE) activity were determined in the brain. The extract characterization was presented using liquid chromatography-mass spectrometry (LC-MS), which confirmed the presence of quercetin, myricetin, kaempferol, catechin, ellagic acid, and gallic acid derivatives. According to the results, the extract at all doses could significantly recover the impairment of cognitive performance of the scopolamine-treated rats. In the MWM test, the extract and rivastigmine reduced escape latency and travelled distance, compared to the scopolamine group. Moreover, in the PA test, the latency to enter the dark chamber was significantly increased by the extract and rivastigmine, compared to the scopolamine group (p<0.05-p<0.001). Similar to rivastigmine, the extract attenuated both AChE activity and oxidative injury in the brain as evidenced by the increased antioxidant enzymes and total thiol content, but it decreased malondialdehyde level (p<0.05-p<0.001). In conclusion, the results suggested the effectiveness of S. minor on preventing cognitive dysfunction induced by scopolamine. Accordingly, these protective effects might be produced through the regulation of cholinergic activity and oxidative stress.

scholarly journals Local Epidermal Endocrine Estrogen Protects Human Melanocytes against Oxidative Stress, a Novel Insight into Vitiligo Pathology

2020 ◽  
Vol 22 (1) ◽  
pp. 269
Author(s):  
Asako Yamamoto ◽  
Lingli Yang ◽  
Yasutaka Kuroda ◽  
Jiao Guo ◽  
Lanting Teng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Estrogen Receptor ◽  
The Body ◽  
Receptor Expression ◽  
Epidermal Keratinocytes ◽  
Protective Effects ◽  
Skin Cells ◽  
Healthy Donors ◽  
Estrogen Synthesis ◽  
Current Therapies

As the outermost barrier of the body, skin is a major target of oxidative stress. In the brain, estrogen has been reported synthesized locally and protects neurons from oxidative stress. Here, we explored whether estrogen is also locally synthesized in the skin to protect from oxidative stress and whether aberrant local estrogen synthesis is involved in skin disorders. Enzymes and estrogen receptor expression in skin cells were examined first by quantitative real-time PCR and Western blot analyses. Interestingly, the estrogen synthesis enzyme was mainly localized in epidermal keratinocytes and estrogen receptors were mainly expressed in melanocytes among 13 kinds of cultured human skin cells. The most abundant estrogen synthesis enzyme expressed in the epidermis was 17β-hydroxysteroid dehydrogenase 1 (HSD17β1) localized in keratinocytes, and the most dominant estrogen receptor expressed in the epidermis was G protein-coupled estrogen receptor 1 (GPER1) in melanocytes. To investigate whether keratinocyte-derived estradiol could protect melanocytes from oxidative stress, cultured human primary epidermal melanocytes (HEMn-MPs) were treated with H2O2 in the presence or absence of 17β estradiol or co-cultured with HSD17β1 siRNA-transfected keratinocytes. Keratinocyte-derived estradiol exhibited protective effects against H2O2-induced cell death. Further, reduced expression of HSD17β1 in the epidermis of skin from vitiligo patients was observed compared to the skin from healthy donors or in the normal portions of the skin in vitiligo patients. Our results suggest a possible new target for interventions that may be used in combination with current therapies for patients with vitiligo.

Protective effects of soy-isoflavones in cardiovascular disease

2008 ◽  
Vol 28 (01/02) ◽  
pp. 85-88 ◽  
Author(s):  
D. Fuchs ◽  
H. Daniel ◽  
U. Wenzel
Keyword(s):  
Cardiovascular Disease ◽  
Endothelial Cells ◽  
Molecular Mechanisms ◽  
Dietary Intervention ◽  
Mononuclear Cells ◽  
Oxidized Ldl ◽  
Proteome Analysis ◽  
Soy Isoflavones ◽  
Protective Effects ◽  
Estrogen Production

SummaryEpidemiological studies indicate that the consumption of soy-containing food may prevent or slow-down the development of cardiovascular disease. In endothelial cells application of a soy extract or a combination of the most abundant soy isoflavones genistein and daidzein both inhibited apoptosis, a driving force in atherosclerosis development, when applied in combination with oxidized LDL or homocysteine. Proteome analysis revealed that the stressorinduced alteration of protein expression profile was reversed by the soy extract or the genistein/daidzein mixture. Only few protein entities that could be functionally linked to mitochondrial dysfunction were regulated in common by both application forms of isoflavones. A dietary intervention with isoflavone-enriched soy extract in postmenopausal women, who generally show strongly increased cardiovascular risk due to diminished estrogen production, led to significant alterations in the steady state levels of proteins from mononuclear blood cells. The proteins identified by proteome analysis revealed that soy isoflavones may increase the anti-inflammatory response in blood mononuclear cells thereby contributing to the atherosclerosispreventive activities of a soy-rich diet. Conclusion: By proteome analysis protein targets were identified in vitro in endothelial cells that respond to soy isoflavones and that may decipher molecular mechanisms through which soy products exert their protective effects in the vasculature.

Sign in / Sign up

Export Citation Format

Share Document