scholarly journals Identification of Novel Single Chain Fragment Variable Antibodies Against TNF-α Using Phage Display Technology

2015 ◽  
Vol 5 (Suppl 1) ◽  
pp. 661-666 ◽  
Author(s):  
Ali Akbar Alizadeh ◽  
Maryam Hamzeh-Mivehroud ◽  
Siavoush Dastmalchi
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Phage Display Technology ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Tnf Α ◽  
Display Technology ◽  
Single Chain Fragment

Isolation and characterization of anti ROR1 single chain fragment variable antibodies using phage display technique

2017 ◽  
Vol 25 (1-2) ◽  
pp. 57-63 ◽  
Author(s):  
Leili Aghebati-Maleki ◽  
Vahid Younesi ◽  
Farhad Jadidi-Niaragh ◽  
Behzad Baradaran ◽  
Jafar Majidi ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Chain Fragment ◽  
Phage Display Technique ◽  
Isolation And Characterization ◽  
Single Chain Fragment Variable ◽  
Display Technique ◽  
Single Chain Fragment

Cloning antifungal single chain fragment variable antibodies by phage display and competitive panning elution

2009 ◽  
Vol 395 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Senthilkumar Krishnaswamy ◽  
M. Enamul Kabir ◽  
Masahiko Miyamoto ◽  
Yasuhiro Furuichi ◽  
Tadazumi Komiyama
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Single Chain Fragment

scholarly journals Production and Purification of a Novel Anti-TNF-α Single Chain Fragment Variable Antibody

2015 ◽  
Vol 5 (Suppl 1) ◽  
pp. 667-672 ◽  
Author(s):  
Ali Akbar Alizadeh ◽  
Maryam Hamzeh-Mivehroud ◽  
Siavoush Dastmalchi
Keyword(s):  
Single Chain ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Tnf Α ◽  
Single Chain Fragment

scholarly journals Human anti-nucleolin recombinant immunoagent for cancer therapy

2015 ◽  
Vol 112 (30) ◽  
pp. 9418-9423 ◽  
Author(s):  
Dario Palmieri ◽  
Timothy Richmond ◽  
Claudia Piovan ◽  
Tyler Sheetz ◽  
Nicola Zanesi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Surface ◽  
Tumor Development ◽  
Target Cells ◽  
Single Chain ◽  
Phage Display Technology ◽  
Chain Fragment ◽  
Mrna Stabilization ◽  
Single Chain Fragment Variable ◽  
Single Chain Fragment

Nucleolin (NCL) is a nucleocytoplasmic protein involved in many biological processes, such as ribosomal assembly, rRNA processing, and mRNA stabilization. NCL also regulates the biogenesis of specific microRNAs (miRNAs) involved in tumor development and aggressiveness. Interestingly, NCL is expressed on the surface of actively proliferating cancer cells, but not on their normal counterparts. Therefore, NCL is an attractive target for antineoplastic treatments. Taking advantage of phage-display technology, we engineered a fully human single-chain fragment variable, named 4LB5. This immunoagent binds NCL on the cell surface, it is translocated into the cytoplasm of target cells, and it abrogates the biogenesis of NCL-dependent miRNAs. Binding of 4LB5 to NCL on the cell surface of a variety of breast cancer and hepatocellular carcinoma cell lines, but not to normal-like MCF-10a breast cells, dramatically reduces cancer cell viability and proliferation. Finally, in orthotopic breast cancer mouse models, 4LB5 administration results in a significant reduction of the tumor volume without evident side effects. In summary, here we describe, to our knowledge, the first anti-NCL single-chain fragment variable displaying antineoplastic activity against established solid tumors, which could represent the prototype of novel immune-based NCL-targeting drugs with clinical potential as diagnostic and therapeutic tools in a wide variety of human cancers.

scholarly journals In Vivo Human Single‐Chain Fragment Variable Phage Display‐Assisted Identification of Galectin‐3 as a New Biomarker of Atherosclerosis

2021 ◽  
Vol 10 (19) ◽  
Author(s):  
Audrey Hemadou ◽  
Alexandre Fontayne ◽  
Jeanny Laroche‐Traineau ◽  
Florence Ottones ◽  
Philippe Mondon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phage Display ◽  
Great Promise ◽  
Single Chain ◽  
Chain Fragment ◽  
Plaque Disruption ◽  
Single Chain Fragment Variable ◽  
Galectin 3 ◽  
Single Chain Fragment

Background Atherosclerosis is a complex pathology in which dysfunctional endothelium, activated leucocytes, macrophages, and lipid‐laden foam cells are implicated, and in which plaque disruption is driven by many putative actors. This study aimed to identify accurate targetable biomarkers using new in vivo approaches to propose tools for improved diagnosis and treatment. Methods and Results Human scFv (single‐chain fragment variable) selected by in vivo phage display in a rabbit model of atherosclerosis was reformatted as scFv fused to the scFv‐Fc (single‐chain fragment variable fused to the crystallizable fragment of immunoglobulin G format) antibodies. Their reactivity was tested using flow cytometry and immunoassays, and aorta sections from animal models and human carotid and coronary artery specimens. A pool of atherosclerotic proteins from human endarterectomies was co‐immunoprecipitated with the selected scFv‐Fc followed by mass spectrometry for target identification. Near‐infrared fluorescence imaging was performed in Apoe −/− mice after injection of an Alexa Fluor 647–labeled scFv‐Fc‐2c antibody produced in a baculovirus system with 2 additional cysteine residues (ie, 2c) for future coupling to nano‐objects for theranostic applications. One scFv‐Fc clone (P3) displayed the highest cross‐reactivity against atherosclerotic lesion sections (rabbit, mouse, and human) and was chosen for translational development. Mass spectrometry identified galectin‐3, a β‐galactoside‐binding lectin, as the leader target. ELISA and immunofluorescence assays with a commercial anti‐galectin‐3 antibody confirmed this specificity. P3 scFv‐Fc‐2c specifically targeted atherosclerotic plaques in the Apoe −/− mouse model. Conclusions These results provide evidence that the P3 antibody holds great promise for molecular imaging of atherosclerosis and other inflammatory pathologies involving macrophages. Recently, galectin‐3 was proposed as a high‐value biomarker for the assessment of coronary and carotid atherosclerosis.

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