scholarly journals Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit

2008 ◽  
Vol 339 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Wen Yuan Chung ◽  
Markus Sack ◽  
Rachel Carter ◽  
Holger Spiegel ◽  
Rainer Fischer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phage Display ◽  
Single Chain ◽  
Chain Fragment ◽  
B Subunit ◽  
Conformational Epitopes ◽  
Heat Labile ◽  
Single Chain Fragment Variable ◽  
Enterotoxin B ◽  
Single Chain Fragment

Isolation and characterization of anti ROR1 single chain fragment variable antibodies using phage display technique

2017 ◽  
Vol 25 (1-2) ◽  
pp. 57-63 ◽  
Author(s):  
Leili Aghebati-Maleki ◽  
Vahid Younesi ◽  
Farhad Jadidi-Niaragh ◽  
Behzad Baradaran ◽  
Jafar Majidi ◽  
...  
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Chain Fragment ◽  
Phage Display Technique ◽  
Isolation And Characterization ◽  
Single Chain Fragment Variable ◽  
Display Technique ◽  
Single Chain Fragment

Cloning antifungal single chain fragment variable antibodies by phage display and competitive panning elution

2009 ◽  
Vol 395 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Senthilkumar Krishnaswamy ◽  
M. Enamul Kabir ◽  
Masahiko Miyamoto ◽  
Yasuhiro Furuichi ◽  
Tadazumi Komiyama
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Single Chain Fragment

scholarly journals In Vivo Human Single‐Chain Fragment Variable Phage Display‐Assisted Identification of Galectin‐3 as a New Biomarker of Atherosclerosis

2021 ◽  
Vol 10 (19) ◽  
Author(s):  
Audrey Hemadou ◽  
Alexandre Fontayne ◽  
Jeanny Laroche‐Traineau ◽  
Florence Ottones ◽  
Philippe Mondon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phage Display ◽  
Great Promise ◽  
Single Chain ◽  
Chain Fragment ◽  
Plaque Disruption ◽  
Single Chain Fragment Variable ◽  
Galectin 3 ◽  
Single Chain Fragment

Background Atherosclerosis is a complex pathology in which dysfunctional endothelium, activated leucocytes, macrophages, and lipid‐laden foam cells are implicated, and in which plaque disruption is driven by many putative actors. This study aimed to identify accurate targetable biomarkers using new in vivo approaches to propose tools for improved diagnosis and treatment. Methods and Results Human scFv (single‐chain fragment variable) selected by in vivo phage display in a rabbit model of atherosclerosis was reformatted as scFv fused to the scFv‐Fc (single‐chain fragment variable fused to the crystallizable fragment of immunoglobulin G format) antibodies. Their reactivity was tested using flow cytometry and immunoassays, and aorta sections from animal models and human carotid and coronary artery specimens. A pool of atherosclerotic proteins from human endarterectomies was co‐immunoprecipitated with the selected scFv‐Fc followed by mass spectrometry for target identification. Near‐infrared fluorescence imaging was performed in Apoe −/− mice after injection of an Alexa Fluor 647–labeled scFv‐Fc‐2c antibody produced in a baculovirus system with 2 additional cysteine residues (ie, 2c) for future coupling to nano‐objects for theranostic applications. One scFv‐Fc clone (P3) displayed the highest cross‐reactivity against atherosclerotic lesion sections (rabbit, mouse, and human) and was chosen for translational development. Mass spectrometry identified galectin‐3, a β‐galactoside‐binding lectin, as the leader target. ELISA and immunofluorescence assays with a commercial anti‐galectin‐3 antibody confirmed this specificity. P3 scFv‐Fc‐2c specifically targeted atherosclerotic plaques in the Apoe −/− mouse model. Conclusions These results provide evidence that the P3 antibody holds great promise for molecular imaging of atherosclerosis and other inflammatory pathologies involving macrophages. Recently, galectin‐3 was proposed as a high‐value biomarker for the assessment of coronary and carotid atherosclerosis.

Production of single chain fragment variable (scFv) antibodies in Escherichia coli using the LEX™ bioreactor

2013 ◽  
Vol 163 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Sebastian Miethe ◽  
Torsten Meyer ◽  
Stefanie Wöhl-Bruhn ◽  
André Frenzel ◽  
Thomas Schirrmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Chain ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Single Chain Fragment
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