Fabrication and properties of poly(L-lactide)/hydroxyapatite/chitosan fiber ternary composite scaffolds for bone tissue engineering

2012 ◽  
Vol 32 (4-5) ◽  
pp. 283-289 ◽  
Author(s):  
Jianhao Zhao ◽  
Chunhong Luo ◽  
Wanqing Han ◽  
Mei Tu ◽  
Rong Zeng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Matrix Material ◽  
Composite Scaffolds ◽  
Ternary Composite ◽  
Electron Microscopy Analysis ◽  
Chitosan Fiber

Abstract A ternary composite scaffold of poly(L-lactide)/hydroxyapatite/chitosan fibers was fabricated for bone tissue engineering. Scanning electron microscopy analysis showed that hydroxyapatite particles and chitosan fibers in microscale uniformly distributing in poly(L-lactide) matrix not only increased the mechanical property but also prevented the crack and platelet formation on the pore surface of poly(L-lactide) matrix at cooling. The X-ray diffraction peak intensity of poly(L-lactide) component decrease may be due to the presence of hydroxyapatite particles and chitosan fibers as the crystal nuclei that led to the crystallinity decrease of poly(L-lactide). The in vitro degradation test showed that the degradation of poly(L-lactide) was resisted by enhancing the chitosan fiber content. The bone mesenchymal stem cell culture indicated that the composite scaffolds promoted the attachment and proliferation of cells, which would likely attach to and stretch along the chitosan fibers. Therefore, this ternary composite scaffold will be a promising matrix material for bone tissue engineering.

scholarly journals In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering

RSC Advances ◽  
2020 ◽  
Vol 10 (40) ◽  
pp. 23813-23828
Author(s):  
Muhammad Rizwan ◽  
Krishnamurithy Genasan ◽  
Malliga Raman Murali ◽  
Hanumantha Rao Balaji Raghavendran ◽  
Rodianah Alias ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Composite Scaffolds ◽  
In Vitro Evaluation ◽  
Monocyte Migration ◽  
Spark Plasma

HB 30 S composite scaffold inhibits Staphylococcus spp., supports the biocompatibility and osteogenic differentiation of hBMSCs and resists monocyte migration.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

scholarly journals Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded nHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Ran Zhang ◽  
Xuewen Li ◽  
Yao Liu ◽  
Xiaobo Gao ◽  
Tong Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Marrow Mesenchymal Stem Cells

Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to develop and characterize a nano-hydroxyapatite (nHA)/collagen I (ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold loaded with recombinant bone morphogenetic protein-9 (BMP-9) for bone tissue engineering by in vitro and in vivo experiments. The composite nHA/ColI/MWCNT scaffolds were fabricated at various concentrations of MWCNTs (0.5, 1, and 1.5% wt) by blending and freeze drying. The porosity, swelling rate, water absorption rate, mechanical properties, and biocompatibility of scaffolds were measured. After loading with BMP-9, bone marrow mesenchymal stem cells (BMMSCs) were seeded to evaluate their characteristics in vitro and in a critical sized defect in Sprague-Dawley rats in vivo. It was shown that the 1% MWCNT group was the most suitable for bone tissue engineering. Our results demonstrated that scaffolds loaded with BMP-9 promoted differentiation of BMMSCs into osteoblasts in vitro and induced more bone formation in vivo. To conclude, nHA/ColI/MWCNT scaffolds loaded with BMP-9 possess high biocompatibility and osteogenesis and are a good candidate for use in bone tissue engineering.

scholarly journals Characterization and osteogenic evaluation of mesoporous magnesium–calcium silicate/polycaprolactone/polybutylene succinate composite scaffolds fabricated by rapid prototyping

RSC Advances ◽  
2018 ◽  
Vol 8 (59) ◽  
pp. 33882-33892 ◽  
Author(s):  
Yun Gyeong Kang ◽  
Jie Wei ◽  
Ji Eun Kim ◽  
Yan Ru Wu ◽  
Eun Jin Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Rapid Prototyping ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Silicate ◽  
Composite Scaffold ◽  
Composite Scaffolds ◽  
Polybutylene Succinate

A new composite scaffold consisting of mesoporous magnesium–calcium silicate (m_MCS), polycaprolactone (PCL), and polybutylene succinate (PBSu) was manufactured by a rapid prototyping technique, for stem cell-based bone tissue engineering.

scholarly journals Development of Fe3O4 integrated polymer/phosphate glass composite scaffolds for bone tissue engineering

2020 ◽  
Vol 1 (9) ◽  
pp. 3466-3475
Author(s):  
Raji Govindan ◽  
Sekar Karthi ◽  
Govindan Suresh Kumar ◽  
Easwaradas Kreedapathy Girija
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Phosphate Glass ◽  
Freeze Drying ◽  
Composite Scaffold ◽  
Glass Composite ◽  
Composite Scaffolds

A multifunctional Fe3O4 integrated polymer/phosphate glass composite scaffold is developed using a freeze drying technique for tissue engineering.

In vitro degradation and bioactivity of poly(propylene fumarate)/bioactive glass sintered microsphere scaffolds for bone tissue engineering

2016 ◽  
Vol 23 (3) ◽  
pp. 245-256 ◽  
Author(s):  
Sima Shahabi ◽  
Yashar Rezaei ◽  
Fathollah Moztarzadeh ◽  
Farhood Najafi
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
P Uptake ◽  
Surface Reactivity ◽  
Ion Concentration ◽  
Composite Scaffolds ◽  
Poly Propylene

AbstractWe developed degradable poly(propylene fumarate)/bioactive glass (PPF/BG) composite scaffolds based on a sintered microsphere technique and investigated the effects of BG content on the characteristics of these composite scaffolds. Immersion in a simulated body fluid (SBF) was used to evaluate the surface reactivity of composite scaffolds. The surface of composite scaffolds was covered with hydroxycarbonate apatite layer after 7 days of immersion. Ion concentration analyses revealed a decrease in P concentration and an increase in Si, Ca, and Sr concentrations in SBF immersed with composite scaffolds during the 3-week period. The Ca and P uptake rates decreased after 4 days of incubation. This coincided with the decrease of the Si release rate. These data lend support to the suggestion that the Si released from the BG content of scaffolds present in the polymer matrix was involved in the formation of the Ca-P layer. The evaluation of the in vitro degradation of composite microspheres revealed that the weight of scaffolds remained relatively constant during the first 3 weeks and then started to decrease slowly, losing 10.5% of their initial mass by week 12. Our results support the concept that these new bioactive, degradable composite scaffolds may be used for bone tissue engineering applications.

Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110557-110565 ◽  
Author(s):  
Yinxian Yu ◽  
Sha Hua ◽  
Mengkai Yang ◽  
Zeze Fu ◽  
Songsong Teng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Tissue Engineering Scaffold ◽  
Fabrication And Characterization

A composite scaffold was fabricated with a method involving both electrospinning and 3D printing to give microscale pores and good mechanical properties. Biocompatibility and cell infiltration on the scaffold was evaluated by an in vitro study.

scholarly journals In VitroDegradation of PHBV Scaffolds and nHA/PHBV Composite Scaffolds Containing Hydroxyapatite Nanoparticles for Bone Tissue Engineering

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Naznin Sultana ◽  
Tareef Hayat Khan
Keyword(s):  
Tissue Engineering ◽  
Weight Loss ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Initial Period ◽  
Tissue Engineering Application ◽  
High Porosity ◽  
Composite Scaffolds

This paper investigated the long-termin vitrodegradation properties of scaffolds based on biodegradable polymers and osteoconductive bioceramic/polymer composite materials for the application of bone tissue engineering. The three-dimensional porous scaffolds were fabricated using emulsion-freezing/freeze-drying technique using poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) which is a natural biodegradable and biocompatible polymer. Nanosized hydroxyapatite (nHA) particles were successfully incorporated into the PHBV scaffolds to render the scaffolds osteoconductive. The PHBV and nHA/PHBV scaffolds were systematically evaluated using various techniques in terms of mechanical strength, porosity, porous morphology, andin vitrodegradation. PHBV and nHA/PHBV scaffolds degraded over time in phosphate-buffered saline at 37°C. PHBV polymer scaffolds exhibited slow molecular weight loss and weight loss in thein vitrophysiological environment. Accelerated weight loss was observed in nHA incorporated PHBV composite scaffolds. An increasing trend of crystallinity was observed during the initial period of degradation time. The compressive properties decreased more than 40% after 5-monthin vitrodegradation. Together with interconnected pores, high porosity, suitable mechanical properties, and slow degradation profile obtained from long-term degradation studies, the PHBV scaffolds and osteoconductive nHA/PHBV composite scaffolds showed promises for bone tissue engineering application.

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