Rocket Electrophoresis

2016 ◽  
Author(s):  
Douglas M. Templeton ◽  
Michael Schwenk ◽  
Reinhild Klein ◽  
John H. Duffus
Keyword(s):  
Rocket Electrophoresis

A reversed rocket electrophoresis method for the determination of high tetanus antitoxin levels

1981 ◽  
Vol 9 (4) ◽  
pp. 367-371 ◽  
Author(s):  
M.E. Rubin ◽  
E. Wong ◽  
H. Bell ◽  
J.M. Bowman ◽  
W.L. Albritton
Keyword(s):  
Tetanus Antitoxin ◽  
Electrophoresis Method ◽  
Rocket Electrophoresis

Preparation of soluble apolipoproteins A-I, B, and C-II by a chromatofocusing column method, and evaluation of their concentrations in serum in pulmonary disease.

1983 ◽  
Vol 29 (10) ◽  
pp. 1731-1735 ◽  
Author(s):  
M Jauhiainen ◽  
M Laitinen ◽  
J Marniemi ◽  
K Liippo ◽  
I Penttilä ◽  
...  
Keyword(s):  
Pulmonary Disease ◽  
Lung Resection ◽  
Water Soluble ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Resection Group ◽  
Column Method ◽  
Rocket Electrophoresis ◽  
Assay Precision ◽  
Interassay Precision

Abstract A chromatofocusing column method for isolating ApoB is described. LDL is first isolated by sequential ultracentrifugation and delipidated with n-butanol/diisopropyl ether. Chromatofocusing of ApoLDL yielded a large ApoB peak at pI 5.0-5.3. ApoA-I and ApoC-II were prepared analogously, with HDL and VLDL as the source of apoprotein. Antisera were raised in rabbits, and electroimmunoassay techniques were used for determination. ApoB was water-soluble after chromatofocusing. Intra-assay precision (CV) was 4.7% for ApoA-I, 7.8% for ApoB in the "rocket" electrophoresis. Interassay precision (CV) was 6% for ApoA-I and 8% for ApoB. Apolipoprotein concentrations were measured in subjects who had undergone lung resection and patients with obstructive pulmonary disease. After lung resection, the concentration of ApoA-I in serum was significantly decreased (p less than 0.001) and that of ApoB significantly increased (p less than 0.001) as compared with controls. The ApoA-I/ApoB ratio was significantly lower in the lung-resection group. ApoA-I and ApoB concentrations were unchanged in chronic obstructive pulmonary disease. ApoC-II concentrations in each group were similar to those for control subjects. Of the lipids, values for total cholesterol were above normal after lung resection (p less than 0.002), as were those for triglycerides (p less than 0.02).

The quantification of chondroitin sulfates by rocket electrophoresis

2005 ◽  
Vol 344 (1) ◽  
pp. 158-160 ◽  
Author(s):  
Jung Hae Yoon ◽  
Jaroslava Halper
Keyword(s):  
Chondroitin Sulfates ◽  
Rocket Electrophoresis

scholarly journals Identification of glycoprotein Ib as a target for autoantibody in idiopathic (autoimmune) thrombocytopenic purpura

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 310-315 ◽  
Author(s):  
NS Szatkowski ◽  
TJ Kunicki ◽  
RH Aster
Keyword(s):  
Direct Evidence ◽  
Protein A ◽  
Normal Subjects ◽  
Platelet Membrane ◽  
Staphylococcal Protein A ◽  
Glycoprotein Ib ◽  
Thrombocytopenic Purpura ◽  
One Dimensional ◽  
Rocket Electrophoresis ◽  
And Control

Abstract An antibody (DIL) from a patient with idiopathic thrombocytopenic purpura (ITP) was shown to have autospecificity on the basis of reactions with autologous platelets that were identical to those obtained with platelets from normal subjects. DIL antibody also reacted strongly in an immunofluorescence test with platelets from a patient with Glanzmann's thrombasthenia, but failed to react with platelets from a patient with the Bernard-Soulier syndrome who was known to be deficient in glycoprotein Ib (GPIb). Purified GPIb and control platelets, but not Bernard-Soulier platelets, inhibited the lytic activity of DIL. Using the GPIb-specific monoclonal antibody AP1 and one-dimensional rocket electrophoresis into gels containing rabbit antihuman platelet membrane antibody, it was shown that staphylococcal protein A-Sepharose beads coated with DIL antibody selectively remove GPIb from solubilized platelet preparations. By crossed immunoelectrophoresis it was found that DIL recognizes a determinant on GPIb on the membrane side of the cleavage site of the platelet calcium- activated protease (calpain). These studies provide direct evidence for binding of a platelet autoantibody to a determinant on GPIb relatively close to the site of insertion of this protein into the platelet membrane.

Raising monospecific antibodies by use of protein components prestained with Remazol Brilliant Blue and separated by disc electrophoresis on polyacrylamide gel.

1984 ◽  
Vol 30 (7) ◽  
pp. 1252-1254 ◽  
Author(s):  
A M Saoji ◽  
C Y Jad ◽  
V L Yemul ◽  
P M Khare ◽  
S S Kelkar
Keyword(s):  
Serum Proteins ◽  
Disc Electrophoresis ◽  
Brilliant Blue ◽  
Direct Visualization ◽  
Crossed Immunoelectrophoresis ◽  
Normal Human ◽  
Rocket Electrophoresis ◽  
Protein Components ◽  
Monospecific Antibodies ◽  
Serum Components

Abstract We have reported (Clin Chem 29: 42-44, 1983) that prestaining with Remazol Brilliant Blue permits direct visualization of serum components on disc electrophoresis, and apparently purifies the proteins well. Here we have cut out the bands corresponding to the prestained albumin and transferrin after disc electrophoresis of normal human serum proteins, eluted some individual proteins into saline, and assessed their purity by immunoelectrophoresis and two-dimensional crossed immunoelectrophoresis against polyvalent antihuman serum. These two techniques indicated purity of these antigens. We inoculated rabbits with the eluates containing the pure antigens, and tested the resulting antibodies for monospecificity by immunoelectrophoresis, rocket electrophoresis, and single radial immunodiffusion. From the results we conclude that the antibodies raised against each component were monospecific, and that this is a simple, economical, rapid, and reliable method for obtaining a pure fraction of serum protein for use as an antigen.

Determination of apolipoprotein A and its constitutive A-I and A-II polypeptides by separate electroimmunoassays.

1976 ◽  
Vol 22 (3) ◽  
pp. 315-322 ◽  
Author(s):  
M D Curry ◽  
P Alaupovic ◽  
C A Suenram
Keyword(s):  
Molar Ratio ◽  
High Density Lipoproteins ◽  
Type I ◽  
Apolipoprotein A ◽  
Present Procedure ◽  
Rocket Electrophoresis ◽  
Coefficients Of Variations ◽  
Monospecific Antisera ◽  
Normal Men

Abstract Electroimmunoassays ("rocket" electrophoresis) are described for human serum apolipoprotein A and its constitutive A-I and A-II polypeptides. Purified lipoprotein A, A-I, and A-II were used to prepare monospecific antisera and to standardize assays. These specific, rapid (5-8 h), precise (the within-and between-assay coefficients of variations are 5 and 7%, respectively), and accurate (by gravimetry) assays are applicable to measurement of these polypeptides in whole serum and in various density classes of lipoproteins. Comparable results are obtained with intact and delipidized lipoproteins. Results correlated well with those obtained by radial immunodiffusion or radioimmunoassay. However, the present procedure is more rapid than the former and simpler than the latter immunoassay. Concentrations of A-I and A-II in the serum of normal men and women were similar (143 +/- 24 and 146 +/- 78 mg/dl, respectively, for A-I and 78 +/- 17 and 83 +/- 25 mg/dl for A-II). Subjects with type lla, llb, and IV hyperlipoproteinemias had similar concentrations of both polypeptides, while patients with type I disease, lecithin:cholesterol acyltransferase deficiency and LP-A deficiency had lowest concentrations of A-I (0.3-30 mg/dl) and A-II (11-20 mg/dl). The molar ratio of A-I/A-II in the serum and high-density lipoproteins was close to unity.

IgG, IgA and IgM by Formylated Rocket Immunoelectrophoresis

1975 ◽  
Vol 12 (1-6) ◽  
pp. 19-24 ◽  
Author(s):  
Linda Slater
Keyword(s):  
Correlation Coefficients ◽  
Specific Protein ◽  
Radial Immunodiffusion ◽  
Single Radial Immunodiffusion ◽  
Serum Immunoglobulins ◽  
Rocket Electrophoresis ◽  
Better Than

Formylated rocket electrophoresis has been investigated as a means of measuring the serum immunoglobulins IgG, IgA, and IgM. This procedure is simpler to carry out than carbamylation. Comparison of formylated rocket results with those from the automated immunoprecipitin (A.I.P.) system and single radial immunodiffusion (S.R.D.) gives correlation coefficients of 0.92 to 0.98 and reproducibility comparable with the A.I.P. system and better than S.R.D. The procedure is recommended for use in laboratories when the number of requests for immunoglobulins does not warrant a specific protein analyser.

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