Lignocellulosic biorefinery: process integration of hydrolysis and fermentation (SSF process)

Holzforschung ◽  
2011 ◽  
Vol 65 (5) ◽  
Author(s):  
Sebastian Poth ◽  
Magaly Monzon ◽  
Nils Tippkötter ◽  
Roland Ulber
Keyword(s):  
Process Integration ◽  
Beech Wood ◽  
Pachysolen Tannophilus ◽  
Process Step ◽  
Fiber Fraction ◽  
Single Process ◽  
Pre Treatment ◽  
Ssf Process ◽  
Hydrolysis Of ◽  
Simultaneous Saccharification

Abstract The aim of the present work is the process integration and the optimization of the enzymatic hydrolysis of wood and the following fermentation of the products to ethanol. The substrate is a fiber fraction obtained by organosolv pre-treatment of beech wood. For the ethanol production, a co-fermentation by two different yeasts (Saccharomyces cerevisiae and Pachysolen tannophilus) was carried out to convert glucose as well as xylose. Two approaches has been followed: 1. A two step process, in which the hydrolysis of the fiber fraction and the fermentation to product are separated from each other. 2. A process, in which the hydrolysis and the fermentation are carried out in one single process step as simultaneous saccharification and fermentation (SSF). Following the first approach, a yield of about 0.15 g ethanol per gram substrate can be reached. Based on the SSF, one process step can be saved, and additionally, the gained yield can be raised up to 0.3 g ethanol per gram substrate.

Cold hydrolysis of cassava pulp and its use in simultaneous saccharification and fermentation (SSF) process for ethanol fermentation

2019 ◽  
Vol 292 ◽  
pp. 57-63 ◽  
Author(s):  
Tanyaporn Siriwong ◽  
Bustomi Laimeheriwa ◽  
Uyun Nurul Aini ◽  
Muhammad Nur Cahyanto ◽  
Alissara Reungsang ◽  
...  
Keyword(s):  
Ethanol Fermentation ◽  
Simultaneous Saccharification And Fermentation ◽  
Cassava Pulp ◽  
Ssf Process ◽  
Hydrolysis Of ◽  
Simultaneous Saccharification

Application of enzymes in anaerobic digestion

2007 ◽  
Vol 56 (10) ◽  
pp. 29-35 ◽  
Author(s):  
G. Bochmann ◽  
T. Herfellner ◽  
F. Susanto ◽  
F. Kreuter ◽  
G. Pesta
Keyword(s):  
Anaerobic Digestion ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Economic Value ◽  
Spent Grain ◽  
Stable Performance ◽  
Pre Treatment ◽  
Ssf Process ◽  
Hydrolysis Of ◽  
Enzyme Application

Owing to the very low economic value of brewer's spent grains, its utilisation for biogas production is very promising. The hydrolysis of ligno-cellulose is the rate limiting step in anaerobic digestion. Enzymatic pre-treatment promotes the hydrolysis of ligno-cellulose, breaking it down to lower molecular weight substances which are ready to be utilised by the bacteria. A cheap raw multi-enzyme produced by a solid state fermentation (SSF) process is a good substitute for expensive conventional enzyme. The SSF enzyme application to spent grain has been investigated by carrying out enzymatic solubility tests, hydrolytic experiments and two-step anaerobic fermentation of spent grain. Gas chromatograph analysis was conducted to quantify fatty acids concentrations, while CH4, CO2, O2, H2 and H2S were measured to determine biogas quality by means of a gas analyser. DS, oDS, pH were also measured to analyse the anaerobic digestion. The result shows that enzyme application promotes the hydrolysis of ligno-cellulose, indicated by higher enzymatic solubility and fatty acid concentration in a hydrolytic bioreactor. Moreover, biogas production is also increased. The quality of the gases produced is also enhanced. Since the anaerobic digestion can be operated in a stable performance, it can also be concluded that SSF enzyme is compatible with anaerobic digestion.

Bioethanol production from thermochemically pre-treated olive mill solid residues using the yeast Pachysolen tannophylus

2013 ◽  
Vol 14 (2) ◽  
pp. 118-124 ◽  
Keyword(s):  
Olive Oil ◽  
Ethanol Yield ◽  
Solid Residue ◽  
Residual Oil ◽  
Edible Fungi ◽  
Pachysolen Tannophilus ◽  
Three Phase ◽  
Different Types ◽  
Pre Treatment ◽  
Olive Mill

Olive oil mill solid residue (OMSR) is the solid waste generated during olive oil production process in three-phase olive mills. It consists of the remaining pulp of olive processing after the extraction of oil, as well as the cracked seeds of the olive fruits, containing thus mainly lignocellulose and residual oil. The commonly used practice for OMSR management is combustion, after having extracted the residual oil by secondary extraction using organic solvents. Other proposed ways of OMSR management are their exploitation as substrate for edible fungi production and compost, and as feedstock for biofuels generation such as methane and bioethanol. In the latter case, the complex carbohydrates (cellulose and hemicellulose) of the lignocellulose of OMSR have to be degraded towards their simple sugars and further fermented via microorganisms. The purpose of the present study was to investigate the effect of thermochemical pre-treatment of OMSR, on the final ethanol yield from the yeast Pachysolen tannophilus. Nine different types of OMSR-based substrates were tested i.e. raw OMSR, hydrolysates generated from pretreated OMSR with NaOH (0.5 %, 1.5 % w/v) and H2SO4 (0.5 %, 1.5 % v/v), and pretreated OMSR with NaOH (0.5 %, 1.5 % w/v) and H2SO4 (0.5 %, 1.5 % v/v) whole biomass. It was shown that in all cases pretreatment enhanced the consumption of carbohydrates as well as ethanol final yields.

Enzyme-assisted Extraction for Optimized Recovery of Phenolic Bioactives from Peganum hermala Leaves Using Response Surface Methodology

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Coumaric Acid ◽  
Assisted Extraction ◽  
Pre Treatment ◽  
Optimized Conditions ◽  
Enzyme Complexes ◽  
Hydrolysis Of ◽  
Central Composite

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.

scholarly journals Bioethanolic yeasts from dung beetles: tapping the potential of extremophilic yeasts for improvement of lignocellulolytic feedstock fermentation

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Anita Ejiro Nwaefuna ◽  
Karl Rumbold ◽  
Teun Boekhout ◽  
Nerve Zhou
Keyword(s):  
Treatment Process ◽  
Dung Beetles ◽  
Production Costs ◽  
Industrial Wastes ◽  
Major Drawback ◽  
Renewable Fuel ◽  
Extreme Stress ◽  
Pre Treatment ◽  
The Cost ◽  
Simultaneous Saccharification

AbstractBioethanol from abundant and inexpensive agricultural and industrial wastes possesses the potential to reduce greenhouse gas emissions. Bioethanol as renewable fuel addresses elevated production costs, as well as food security concerns. Although technical advancements in simultaneous saccharification and fermentation have reduced the cost of production, one major drawback of this technology is that the pre-treatment process creates environmental stressors inhibitory to fermentative yeasts subsequently reducing bioethanol productivity. Robust fermentative yeasts with extreme stress tolerance remain limited. This review presents the potential of dung beetles from pristine and unexplored environments as an attractive source of extremophilic bioethanolic yeasts. Dung beetles survive on a recalcitrant lignocellulose-rich diet suggesting the presence of symbiotic yeasts with a cellulolytic potential. Dung beetles inhabiting extreme stress environments have the potential to harbour yeasts with the ability to withstand inhibitory environmental stresses typically associated with bioethanol production. The review further discusses established methods used to isolate bioethanolic yeasts, from dung beetles.

scholarly journals Thermodynamic and Experimental Investigation of Solar-Driven Biomass Pyro-Gasification Using H2O, CO2, or ZnO Oxidants for Clean Syngas and Metallurgical Zn Production

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 687
Author(s):  
Srirat Chuayboon ◽  
Stéphane Abanades
Keyword(s):  
Beech Wood ◽  
Biomass Gasification ◽  
Biomass Energy ◽  
Solid Product ◽  
Product Characterization ◽  
Single Process ◽  
U Value ◽  
Chemical Products ◽  
Particle Shapes ◽  
Promising Avenue

The solar gasification of biomass represents a promising avenue in which both renewable solar and biomass energy can be utilized in a single process to produce synthesis gas. The type of oxidant plays a key role in solar-driven biomass gasification performance. In this study, solar gasification of beech wood biomass with different oxidants was thermodynamically and experimentally investigated in a 1.5 kWth continuously-fed consuming bed solar reactor at 1200 °C under atmospheric pressure. Gaseous (H2O and CO2) as well as solid (ZnO) oxidants in pellet and particle shapes were utilized for gasifying beech wood, and the results were compared with pyrolysis (no oxidant). As a result, thermodynamic predictions provided insights into chemical gasification reactions against oxidants, which can support experimental results. Compared to pyrolysis, using oxidants significantly promoted syngas yield and energy upgrade factor. The highest total syngas yield (63.8 mmol/gbiomass) was obtained from biomass gasification with H2O, followed by CO2, ZnO/biomass mixture (pellets and particles), and pyrolysis. An energy upgrade factor (U) exceeding one was achieved whatever the oxidants, with the maximum U value of 1.09 from biomass gasification with ZnO, thus highlighting successful solar energy storage into chemical products. ZnO/biomass pellets exhibited greater gas yield, particularly CO, thanks to enhanced solid–solid reaction. Solid product characterization revealed that ZnO can be reduced to high-purity Zn through solar gasification, indicating that solar-driven biomass gasification with ZnO is a promising innovative process for CO2-free sustainable co-production of metallic Zn and high-quality syngas.

scholarly journals The effect of drying temperature on the properties of gelatin from carps (Cyprinus carpio) skin

2019 ◽  
Vol 37 (No. 4) ◽  
pp. 246-251 ◽  
Author(s):  
Joanna Tkaczewska ◽  
Maciej Wielgosz ◽  
Piotr Kulawik ◽  
Marzena Zajac
Keyword(s):  
Cyprinus Carpio ◽  
Freeze Drying ◽  
Treatment Method ◽  
Thermal Hydrolysis ◽  
Gel Properties ◽  
Drying Temperature ◽  
Freeze Dried ◽  
Different Temperatures ◽  
Pre Treatment ◽  
Hydrolysis Of

The influence of drying temperature on the characteristics and gel properties of gelatine from Cyprinus carpio L. skin was studied. Gelatine was extracted from the carp skin using NaOH and ethanol pre-treatment method, extracted in water in 45°C and then dried in 4 different temperatures: 50, 70, 80°C and freeze-dried. The  electrophoresis and functional properties of gelatines were investigated. Freeze drying allowed to obtain a high gelling force, and all other methods did not give satisfactory results. The proteins in gelatines dried at higher temperatures separated by electrophoresis gave severely blurred bands. It may be explained by thermal hydrolysis of collagen fibrils. Freeze drying is the only effective method for drying this product, which can be used in industry.

Application of Tem on Sub-Half Micron Semiconductor Devices

1998 ◽  
Vol 523 ◽  
Author(s):  
Hong Zhang
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Failure Analysis ◽  
Process Evaluation ◽  
Semiconductor Devices ◽  
Single Source ◽  
Process Step ◽  
Transmission Electron ◽  
Single Process ◽  
Single Process Step

AbstractApplication of transmission electron microscopy on sub-half micron devices has been illustrated in terms of process evaluation and failure analysis. For process evaluation, it is emphasized that a large number of features need to be examined in order to have reliable conclusions about the processes, while for failure analysis, the goal is to pin-point a single process step causing failure or a single source introducing the particle defect.

Evaluation and development of a novel pre-treatment method for mulberry leaves to enhance their bioactivity via enzymatic degradation of GAL-DNJ to DNJ

2021 ◽  
Author(s):  
Soo Takasu ◽  
Isabella Supardi Parida ◽  
Yoshihiro Kojima ◽  
Toshiyuki Kimura ◽  
Kiyotaka Nakagawa
Keyword(s):  
Enzymatic Degradation ◽  
Treatment Method ◽  
Mulberry Leaves ◽  
Pre Treatment ◽  
Hydrolysis Of

We demonstrated the conversion of GAL-DNJ into DNJ improves mulberry leaves bioavailability and constructed a novel enzymatic-based method to induce the hydrolysis of GAL-DNJ to DNJ.

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