Microbial colonizers of freshly sawn yellow-poplar (Liriodendron tulipifera L.) lumber in two seasons: Part 1. Fungi

Holzforschung ◽  
2004 ◽  
Vol 58 (2) ◽  
pp. 173-181 ◽  
Author(s):  
M. R. Mikluscak ◽  
B. E. Dawson-Andoh
Keyword(s):  
Alternaria Alternata ◽  
Fungal Species ◽  
Aspergillus Versicolor ◽  
Total Abundance ◽  
Yellow Poplar ◽  
Entire Study ◽  
Fungal Isolates ◽  
Hormoconis Resinae ◽  
Time Periods ◽  
Penicillium Implicatum

AbstractThis is the first of a three-paper series that reports on the abundance, identity, and diversity of microorganisms isolated from dead-stacked yellow-poplar lumber in four time periods during the spring and summer of 2000. This paper focuses on filamentous fungi. Over the entire period, a total of 427 fungal isolates were obtained and 14 species representing 12 genera were identified. Nearly 15% of the fungal isolates were not identified. In total, fungal colonies accounted for 41% of the total abundance of microorganisms that were isolated from samples during this entire study.Penicillium implicatumandAspergillus versicolorwere the predominant fungal species identified and accounted for 29.7% and 14.5%, respectively, of the total fungal isolates.Penicilliumsp was the most commonly isolated genera. Other fungi isolated at lower frequencies includeGraphium penicilliodes,Cladosporium cladosporioides,Trichoderma harzium,Alternaria alternata,A. tenuissima,Epicoccum purpurscens,Hormoconis resinae,Fusarium monoilforme,Geotrichumsp,Chaetomium globosum, andExophiala jeanselmi.

Ophiostomatoid Fungi Associated with Cryphalus Piceae in Shandong province in eastern China

2021 ◽  
Author(s):  
Runlei Chang ◽  
Xiuyu Zhang ◽  
Hongli Si ◽  
Guoyan Zhao ◽  
Xiaowen Yuan ◽  
...  
Keyword(s):  
Bark Beetles ◽  
Novel Species ◽  
Eastern China ◽  
Fungal Species ◽  
Shandong Province ◽  
Morphological Data ◽  
Ophiostomatoid Fungi ◽  
Fungal Isolates ◽  
Study Sites ◽  
Isolated Species

Abstract Cryphalus piceae parasitizes various economically important conifers. Similar to other bark beetles, C. picea vectors an assortment of fungi and nematodes. Previously, several ophiostomatoid fungi were isolated from C. piceae in Poland and Japan. In the present study, we explored the diversity of ophiostomatoid fungi associated with C. piceae infesting pines in the Shandong Province of China. We isolated ophiostomatoid fungi from both galleries and beetles collected from our study sites. These fungal isolates were identified using both molecular and morphological data. Through this study, we recovered 176 isolates of ophiostomatoid fungi representing at least seven species. Ophiostoma ips was the most frequently isolated species. Analyses of molecular and morphological data indicated four of the ophiostomatoid fungal species recovered in this study were previously undescribed. Hereby, we described these species as Ceratocystiopsis yantaiensis sp. nov., C. weihaiensis sp. nov., Graphilbum translucens sp. nov. and Sporothrix villosa sp. nov. A majority of the ophiostomatoid fungi recovered in this study were novel species. This suggests that the forests in China harbour an assortment of undescribed ophiostomatoid fungi yet to be discovered.

Screening and Identification of Laccase Producing Fungi from Environmental Samples

2021 ◽  
Vol 6 (1) ◽  
pp. 91-98
Author(s):  
A. Bello ◽  
◽  
J. B. Ameh ◽  
D. A. Machido ◽  
A. I. Mohammed-Dabo
Keyword(s):  
Tannic Acid ◽  
Fungal Species ◽  
Soil Samples ◽  
Potato Dextrose Agar ◽  
Laccase Production ◽  
Curvularia Lunata ◽  
Main Campus ◽  
Fungal Isolates ◽  
Screening And Identification ◽  
Decaying Wood

Laccases are oxidases with broad substrate specificity and ability to oxidize various phenolic and non-phenolic compounds. This study was carried out to isolate and characterizes laccase producing fungi from environment samples. Soil and decaying wood samples were collected from different locations within Ahmadu Bello University, Zaria Main campus. Suspensions of the samples (1 g in 10 mL sterile distilled water) were serially diluted, inoculated onto Potato Dextrose Agar (PDA) containing 0.01% Chloramphenicol and incubated for 7 days at 30oC.The fungal isolates were characterized macroscopically and microscopically with the aid of an atlas. The identified fungal isolates were screened for laccase production by inoculating onto PDA containing 0.02% Guaiacol, 1mM ABTS (2 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and 0.5% Tannic acid as indicator compounds and incubated at 250C for 7 days. The laccase producing isolates were confirmed molecularly by ITS rDNA sequence analysis using the FASTA algorithm with the Fungus database from the European Bioinformatics Institute (EBI).A total of 25 fungal species (11 from soil and 14 from decaying wood samples) were isolated. Two isolates from the soil origin identified as Curvularia lunata SSI7 (Accession No. QIE06317.1) and Fusarium clade VII SSI3 (Accession No. GQ505677) were found to produce laccase where Curvularia lunata SSI7 was able to oxidize all the indicator compounds used for the screening. Fusarium clade VII SSI3 was able to oxidize only 0.5% Tannic acid. Laccase producing Curvularia lunata and Fusarium clade VII were isolated from soil samples collected from ABU Zaria Main Campus. Keywords: laccase, fungi, soil, decaying wood

scholarly journals Mycoflora associated with two species of Liliaceae in Bangladesh

2013 ◽  
Vol 22 (1) ◽  
pp. 39-46
Author(s):  
Shamim Shamsi ◽  
Najmun Naher
Keyword(s):  
Fusarium Solani ◽  
Alternaria Alternata ◽  
New Record ◽  
Macrophomina Phaseolina ◽  
Fungal Species ◽  
Colletotrichum Orbiculare ◽  
Lasiodiplodia Theobromae ◽  
Colletotrichum Capsici ◽  
Colletotrichum Dematium

A total of nine fungi were isolated from two ornamental angiosperms, namely Hemerocallis fulva L. and Pancratium verecundum Ant. belong to Liliaceae. Three species of fungi were found to be associated with the leaf of Hemerocallis fulva. The fungi were Colletotrichum capsici, (Syd.) Bull. & Bisby, Colletotrichum dematium (Pers. Ex Fr.) and Glomerella montana (Sacc.)v Arx & E. Muller. Six species of fungi, namely Alternaria alternata (Fries) Keissler, Colletotrichum orbiculare (Berk & Mont.) Arex., Curvularia clavata Jain, Fusarium solani (Mort.) Sacc., Lasiodiplodia theobromae (Pat.) Griffon and Maubol and Macrophomina phaseolina (Tassi) Goid were found to be associated with P. verecundum. Among the fungal species Glomerella montana is new record for Bangladesh. Dhaka Univ. J. Biol. Sci. 22(1): 39-46, 2013 (January)

scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

2018 ◽  
Vol 11 (1) ◽  
pp. 408
Author(s):  
Kassim Dekhil
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Influencing Factors ◽  
Aspergillus Flavus ◽  
Species Distribution ◽  
Alternaria Alternata ◽  
Otitis Externa ◽  
Fungal Species ◽  
The Public

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

scholarly journals New plectosphaerellaceous species from Dutch garden soil

2019 ◽  
Vol 18 (9) ◽  
pp. 1135-1154 ◽  
Author(s):  
Alejandra Giraldo ◽  
Margarita Hernández-Restrepo ◽  
Pedro W. Crous
Keyword(s):  
New Species ◽  
Citizen Science ◽  
Morphological Characters ◽  
Fungal Species ◽  
Soil Samples ◽  
Garden Soil ◽  
Science Project ◽  
Fungal Isolates ◽  
University Museum ◽  
Citizen Science Project

Abstract During 2017, the Westerdijk Fungal Biodiversity Institute (WI) and the Utrecht University Museum launched a Citizen Science project. Dutch school children collected soil samples from gardens at different localities in the Netherlands, and submitted them to the WI where they were analysed in order to find new fungal species. Around 3000 fungal isolates, including filamentous fungi and yeasts, were cultured, preserved and submitted for DNA sequencing. Through analysis of the ITS and LSU sequences from the obtained isolates, several plectosphaerellaceous fungi were identified for further study. Based on morphological characters and the combined analysis of the ITS and TEF1-α sequences, some isolates were found to represent new species in the genera Phialoparvum, i.e. Ph. maaspleinense and Ph. rietveltiae, and Plectosphaerella, i.e. Pl. hanneae and Pl. verschoorii, which are described and illustrated here.

scholarly journals Mycotoxins in Crude Building Materials from Water-Damaged Buildings

2000 ◽  
Vol 66 (5) ◽  
pp. 1899-1904 ◽  
Author(s):  
Tapani Tuomi ◽  
Kari Reijula ◽  
Tom Johnsson ◽  
Kaisa Hemminki ◽  
Eeva-Liisa Hintikka ◽  
...  
Keyword(s):  
Building Materials ◽  
Growth Conditions ◽  
Fungal Species ◽  
Aspergillus Versicolor ◽  
Chemical Analyses ◽  
Bulk Materials ◽  
Toxigenic Fungi ◽  
Three Samples

ABSTRACT We analyzed 79 bulk samples of moldy interior finishes from Finnish buildings with moisture problems for 17 mycotoxins, as well as for fungi that could be isolated using one medium and one set of growth conditions. We found the aflatoxin precursor, sterigmatocystin, in 24% of the samples and trichothecenes in 19% of the samples. Trichothecenes found included satratoxin G or H in five samples; diacetoxyscirpenol in five samples; and 3-acetyl-deoxynivalenol, deoxynivalenol, verrucarol, or T-2-tetraol in an additional five samples. Citrinine was found in three samples. Aspergillus versicolor was present in most sterigmatocystin-containing samples, and Stachybotrys spp. were present in the samples where satratoxins were found. In many cases, however, the presence of fungi thought to produce the mycotoxins was not correlated with the presence of the expected compounds. However, when mycotoxins were found, some toxigenic fungi usually were present, even if the species originally responsible for producing the mycotoxin was not isolated. We conclude that the identification and enumeration of fungal species present in bulk materials are important to verify the severity of mold damage but that chemical analyses are necessary if the goal is to establish the presence of mycotoxins in moldy materials.

scholarly journals Novel Victorivirus from a Pakistani Isolate of Alternaria alternata Lacking a Typical Translational Stop/Restart Sequence Signature

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 577 ◽  
Author(s):  
Atif Jamal ◽  
Yukiyo Sato ◽  
Sabitree Shahi ◽  
Wajeeha Shamsi ◽  
Hideki Kondo ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Rna Silencing ◽  
Polyacrylamide Gel Electrophoresis ◽  
Peptide Mass Fingerprinting ◽  
Cryphonectria Parasitica ◽  
Fungal Species ◽  
Open Reading Frames ◽  
Fusion Product ◽  
Peptide Mass ◽  
Sequence Signature

The family Totiviridae currently contains five genera Totivirus, Victorivirus, Leishmavirus, Trichomonasvirus, and Giardiavirus. Members in this family generally have a set of two-open reading frame (ORF) elements in their genome with the 5′-proximal ORF (ORF1) encoding a capsid protein (CP) and the 3′-proximal one (ORF2) for RNA-dependent RNA polymerase (RdRp). How the downstream open reading frames (ORFs) are expressed is genus-specific. All victoriviruses characterized thus far appear to use the stop/restart translation mechanism, allowing for the expression of two separate protein products from bicitronic genome-sized viral mRNA, while the totiviruses use a −1 ribosomal frame-shifting that leads to a fusion product of CP and RdRp. We report the biological and molecular characterization of a novel victorivirus termed Alternaria alternata victorivirus 1 (AalVV1) isolated from Alternaria alternata in Pakistan. The phylogenetic and molecular analyses showed AalVV1 to be distinct from previously reported victoriviruses. AalVV1 appears to have a sequence signature required for the −1 frame-shifting at the ORF1/2 junction region, rather than a stop/restart key mediator. By contrast, SDS–polyacrylamide gel electrophoresis and peptide mass fingerprinting analyses of purified virion preparations suggested the expression of two protein products, not a CP-RdRp fusion product. How these proteins are expressed is discussed in this study. Possible effects of infection by this virus were tested in two fungal species: A. alternata and RNA silencing proficient and deficient strains of Cryphonectria parasitica, a model filamentous fungus. AalVV1 showed symptomless infection in all of these fungal strains, even in the RNA silencing deficient C. parasitica strain.

scholarly journals Analysis of the spatial variation in the parameters of the SWAT model with application in Flanders, Northern Belgium

2004 ◽  
Vol 8 (5) ◽  
pp. 931-939 ◽  
Author(s):  
G. Heuvelmans ◽  
B. Muys ◽  
J. Feyen
Keyword(s):  
Stream Flow ◽  
Hydrological Model ◽  
Swat Model ◽  
Single Parameter ◽  
Model Parameters ◽  
Study Region ◽  
Entire Study ◽  
Time Periods ◽  
Parameter Values ◽  
Parameter Approach

Abstract. Operational applications of a hydrological model often require the prediction of stream flow in (future) time periods without stream flow observations or in ungauged catchments. Data for a case-specific optimisation of model parameters are not available for such applications, so parameters have to be derived from other catchments or time periods. It has been demonstrated that for applications of the SWAT in Northern Belgium, temporal transfers of the parameters have less influence than spatial transfers on the performance of the model. This study examines the spatial variation in parameter optima in more detail. The aim was to delineate zones wherein model parameters can be transferred without a significant loss of model performance. SWAT was calibrated for 25 catchments that are part of eight larger sub-basins of the Scheldt river basin. Two approaches are discussed for grouping these units in zones with a uniform set of parameters: a single parameter approach considering each parameter separately and a parameter set approach evaluating the parameterisation as a whole. For every catchment, the SWAT model was run with the local parameter optima, with the average parameter values for the entire study region (Flanders), with the zones delineated with the single parameter approach and with the zones obtained by the parameter set approach. Comparison of the model performances of these four parameterisation strategies indicates that both the single parameter and the parameter set zones lead to stream flow predictions that are more accurate than if the entire study region were treated as one single zone. On the other hand, the use of zonal average parameter values results in a considerably worse model fit compared to local parameter optima. Clustering of parameter sets gives a more accurate result than the single parameter approach and is, therefore, the preferred technique for use in the parameterisation of ungauged sub-catchments as part of the simulation of a large river basin. Keywords: hydrological model, regionalisation, parameterisation, spatial variability

scholarly journals Isolation and identification of common fungal spp. from commercial broiler feeds available in market of Sylhet District, Bangladesh

2016 ◽  
Vol 4 (2) ◽  
pp. 38-41 ◽  
Author(s):  
MT Islam ◽  
MK Hossain ◽  
ATMM Elahi ◽  
M Purkayastha ◽  
MM Rahman
Keyword(s):  
Fungal Species ◽  
Natural Sciences ◽  
Local Market ◽  
Frequency Of Occurrence ◽  
Isolation And Identification ◽  
Fungal Isolates ◽  
Commercial Broiler ◽  
Rhizopus Sp ◽  
Feed Samples ◽  
Broiler Feed

The present study was designed to investigate the mycological contamination of commercial broiler feeds used in poultry establishments in sylhet, Bangladesh. The feed samples of commercial broiler feed (Starter, Grower and Finisher) were collected from the different areas of Sylhet district. A total of 189 commercial broiler feed samples where 63 Starter, 63 Grower and 63 Finisher were collected from the different areas of local market in Sylhet. The selected areas were Kadamtali, Shibjong, Khadim, Kamal Bazar, Dakshin Surma, Fenchugonj. From the feed samples analyzed for the presence of fungal agents, 144 (76.2%) were found positive for one or more fungal species. Fungal isolates were found among 36 (57%) of the 63 Starter feed samples, 45 (71.4%) of the 63 Grower feed samples and 63 (100%) of the 63 Finisher feed samples. The fungal agents isolated from Broiler Starter Feeds, Aspergillus spp. 51 (70.8%) has the highest frequency of occurrence, followed by Fuserium spp. 12 (16.7%) and least is Rhizopus sp. 9(12.5%). Similarly, in case of Broiler Grower Feeds, Aspergillus spp. 66 (68.8%) has the highest frequency of occurrence, followed by Fuserium spp. 18 (18.7%) and least is Rhizopus sp. 12(12.5%). In case of Broiler Finisher Feeds, Aspergillus spp. 90 (69.8%) has the highest rate of occurrence followed by Fuserium spp. 24 (18.6%) and least is Rhizopus sp. 15 (11.6%) respectively.International Journal of Natural Sciences (2014), 4(2) 38-41

Virulence and in vitro Characteristics of Pathogenic Fungi Isolated from Soil by Baiting with Coptotermes formosanus (Isoptera: Rhinotermitidae)

2003 ◽  
Vol 38 (3) ◽  
pp. 342-358 ◽  
Author(s):  
Jianzhong Sun ◽  
James R. Fuxa ◽  
Gregg Henderson
Keyword(s):  
Pathogenic Fungi ◽  
Fungal Species ◽  
Coptotermes Formosanus ◽  
Survival Times ◽  
Fungal Virulence ◽  
Fungal Isolates ◽  
Sampling Locations ◽  
Lethal Doses

Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae) was used as “bait” to isolate pathogenic fungi from soil. Ninety soil samples were collected from woodlands and pastures in the vicinities of Baton Rouge, New Orleans, and Lake Charles, LA, from which six Metarhizium anisopliae (Metsch.) Sorokin and nine Beauveria bassiana (Balsamo) Vuillemin isolates were obtained. Numbers of fungal isolates from the three sampling locations did not differ, but more isolates were found in woodlands than in pastures. Median lethal doses (LD50s) of these fungal species to C. formosanus were interspersed, indicating that fungal isolates rather than species had the greatest effect on virulence. Among nine Louisiana and two USDA isolates of B. bassiana, LD50s ranged from 4.95 × 103 to 4.96 × 105 conidia/termite, a difference of 100×. LD50s of six Louisiana and four USDA isolates of M. anisopliae ranged from 7.89 × 103 to 1.22 × 105 conidia/termite. Survival time also was used to compare virulence; M. anisopliae infections caused significantly shorter host survival times than B. bassiana. In vitro growth characteristics were significantly correlated with virulence against termites, suggesting that the characteristics of a fungus growing on agar might contribute to estimating the fungal virulence in vivo.

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