Ultrastructural Localisation of Glucomannan in Kraft Pulp Fibres

Holzforschung ◽  
2003 ◽  
Vol 57 (1) ◽  
pp. 62-68 ◽  
Author(s):  
I. Duchesne ◽  
K. Takabe ◽  
G. Daniel
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Kraft Pulp ◽  
Immunogold Labelling ◽  
Cellulose Microfibrils ◽  
Hemicellulose Content ◽  
Transmission Electron ◽  
Hemicellulose Removal ◽  
Pulp Fibres ◽  
Fibril Aggregates

Summary The localisation of glucomannan on the surface of Norway spruce kraft pulp fibres with varying glucomannan (GM) contents (10.3 and 3.8%) was studied using immunogold labelling, rapid freeze deep etching (RFDE) followed by metal replication and transmission electron microscopy (TEM). The ultrastructure of P, S1 and S2 of pulp fibres with low hemicellulose content tended to be more compact than that of pulp fibres with high hemicellulose content, suggesting aggregation of cellulose microfibrils upon hemicellulose removal. The degree of immunogold labelling visualised as globular structures was similar for the two pulps, despite the large difference in total glucomannan content. Thus, no correlation between the bulk and surface contents of glucomannan was found. The globular structures were heterogeneously located along the cellulose macrofibrils (fibril aggregates) and their frequency varied greatly within and between TEM micrographs.

Immunolocalization of a proteinase of the sap-staining fungus Ophiostoma piceae using antibodies to proteinase K

1995 ◽  
Vol 73 (10) ◽  
pp. 1604-1610 ◽  
Author(s):  
C. Hoffert ◽  
S. Gharibian ◽  
C. Breuil ◽  
D. L. Brown
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Walls ◽  
Polyclonal Antibodies ◽  
Immunogold Labelling ◽  
Proteinase K ◽  
Igg Antibodies ◽  
Extracellular Proteinase ◽  
Ophiostoma Piceae ◽  
Transmission Electron

Polyclonal antibodies were raised against proteinase K and were used to immunolocalize the major extracellular proteinase of the sap-staining fungus Ophiostoma piceae (Münch) H. and P. Sydow. Immunodot blotting showed that the IgG antibodies recognized both enzymes but reacted more strongly with proteinase K than with the O. piceae proteinase. Immunogold labelling and transmission electron microscopy revealed that the O. piceae proteinase was localized in the cell walls of O. piceae grown either in liquid media or wood. Key words: Ophiostoma piceae, proteinase, immunogold labelling, transmission electron microscopy, antibody, proteinase K.

scholarly journals Swelling of Valonia cellulose microfibrils in amine oxide systems

2008 ◽  
Vol 86 (6) ◽  
pp. 520-524 ◽  
Author(s):  
Pierre Noé ◽  
Henri Chanzy
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Electron Diffraction ◽  
Amine Oxide ◽  
Cellulose Microfibrils ◽  
Electron Diffraction Analysis ◽  
Oxide Systems ◽  
Transmission Electron ◽  
Valonia Ventricosa

Cellulose microfibrils from Valonia ventricosa cell-wall fragments were immersed into molten N-methylmorpholine-N-oxide monohydrate (NMMO·H2O), stabilized with n-propyl gallate and kept at 80 °C. The resulting ultrastructural modifications, which were followed by transmission electron microscopy and electron diffraction analysis, showed that within minutes the solvent slowly penetrated inside the crystalline microfibrils and progressed as a wedge in between the cellulose chains without cutting them. Prior to dissolution, a longitudinal subfibrillation of the initial microfibrils occurred, leading to the observation of highly swollen microfibrils, which could reach diameters up to three times larger than those of the initial samples. This mode of swelling is compared with those occurring in other systems, where the intracrystalline swelling of cellulose has been described at the ultrastructural level.Key words: cellulose swelling, Valonia cellulose, N-methylmorpholine-N-oxide.

scholarly journals Immunogold-labelling localization of chlorophyllase at different developmental stages of Pachira macrocarpa leaves

2021 ◽  
Author(s):  
Tzan-Chain Lee ◽  
Kuan-Hung Lin ◽  
Chang-Chang Chen ◽  
Tin-Han Shih ◽  
Meng-Yuan Huang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Plasma Membrane ◽  
Thylakoid Membrane ◽  
Developmental Stages ◽  
Higher Plants ◽  
Plant Cells ◽  
Immunogold Labelling ◽  
Transmission Electron

Abstract Background: Chlorophyllases (Chlases) are housekeeping proteins in plant cells. The dephytylating enzymes can catalyze chlorophyll (Chl) to form chlorophyllide, but the distribution of Chlases in plant cells is still an interesting debate. In this study, antibody of PmCLH2 was made and used by immunogold-labelling technique to detect the location of Chlase of Pachira macrocarpa (Pm) leaves at four developmental stages, including young, mature, yellowing, and senesced stages. Results: The transmission electron microscopy results show that Chlases were comprehensively found in portions of chloroplast, such as the inner membrane of the envelope, grana, and the thylakoid membrane of the chloroplast, cytosol, and vacuoles at young, mature, and yellowing stages of Pm leaves, but not in the cell wall, plasma membrane, mitochondria, and nucleus. Conclusions: PmChlases were mainly detected in vacuoles at the senescent stage, but a few were found in the chloroplasts. A pathway is proposed to explain the birth and death of Chl, Chlase, and chloroplasts in higher plants.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Sign in / Sign up

Export Citation Format

Share Document