Molecular Methods for the Characterization and Identification of the Dry Rot Fungus Serpula lacrymans

Holzforschung ◽  
2000 ◽  
Vol 54 (3) ◽  
pp. 221-228 ◽  
Author(s):  
Olaf Schmidt
Keyword(s):  
Nucleic Acids ◽  
Dna Sequencing ◽  
Hamburg Institute ◽  
Molecular Methods ◽  
International Literature ◽  
Protein Patterns ◽  
Dry Rot ◽  
Oligonucleotide Primers ◽  
Target Molecules ◽  
Serpula Lacrymans

Summary Novel methods developed for characterization and identification of wood-inhabiting fungi target molecules of the organisms such as proteins and nucleic acids and use mycelial interaction, mating, antibodies, electrophoretic protein patterns, oligonucleotide primers and DNA sequencing. The article reviews the respective work on the dry rot fungus Serpula lacrymans in the Hamburg institute and also covers the international literature.

Treatment of Timber by Nanofiber Fabric with Biocide Compound

2014 ◽  
Vol 1000 ◽  
pp. 154-157
Author(s):  
Zuzana Rácová ◽  
Petra Hrochová ◽  
Pavla Ryparová
Keyword(s):  
Dry Rot ◽  
Petri Dishes ◽  
Materials Used ◽  
Serpula Lacrymans ◽  
Filter Papers ◽  
Fungus Growth

Dry rot fungus (Serpula lacrymans) is wood-decaying fungus. It grows frequently in our territory and it causes big damages on structures. Remediation of damaged structures is very difficult, sometimes impossible, therefore it is necessary to study preventive protection against dry rot fungus. PVA nanofibred fabrics with synthetic and natural biocidal additives were used for this experiment. Filter papers soaked in dopes with biocidal substances were other materials used for this experiment. Pieces of nanofiber fabrics and pieces of filter papers soaked in dopes were placed to Petri dishes with broth. Small cuts of dry rot fungus were placed around them. This experiment was performed in conditions, which promote the growth of dry rot fungus. Growth of dry rot fungus was studied.

scholarly journals Ligands with poly-fluorophenyl moieties promote a local structural rearrangement in the Spinach2 and Broccoli aptamers that increases ligand affinities

2021 ◽  
Author(s):  
Sharif Anisuzzaman ◽  
Ivan M Geraskin ◽  
Muslum Ilgu ◽  
Lee Bendickson ◽  
George A Kraus ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Ligand Binding ◽  
Small Molecule ◽  
Binding Pocket ◽  
Structural Rearrangement ◽  
Structural Reorganization ◽  
Ligand Binding Pocket ◽  
Rna Remodeling ◽  
Small Molecule Ligands ◽  
Target Molecules

The interaction of nucleic acids with their molecular targets often involves structural reorganization that may traverse a complex folding landscape. With the more recent recognition that many RNAs, both coding and noncoding, may regulate cellular activities by interacting with target molecules, it becomes increasingly important to understand the means by which nucleic acids interact with their targets and how drugs might be developed that can influence critical folding transitions. We have extensively investigated the interaction of the Spinach2 and Broccoli aptamers with a library of small molecule ligands modified by various extensions from the imido nitrogen of DFHBI (3,5-difluoro-4-hydroxybenzylidene imidazolinone) that reach out from the Spinach2 ligand binding pocket. Studies of the interaction of these compounds with the aptamers revealed that poly-fluorophenyl-modified ligands initiate a slow change in aptamer affinity that takes an extended time (half-life of ~40 min) to achieve. The change in affinity appears to involve an initial disruption of the entrance to the ligand binding pocket followed by a gradual lockdown for which the most likely driving force is an interaction of the gateway adenine with a nearby 2'OH group. These results suggest that poly-fluorophenyl modifications might increase the ability of small molecule drugs to disrupt local structure and promote RNA remodeling.

scholarly journals Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 299 ◽  
Author(s):  
Jinho Yoon ◽  
Minkyu Shin ◽  
Taek Lee ◽  
Jeong-Woo Choi
Keyword(s):  
Nucleic Acids ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Transition Metal Dichalcogenide ◽  
The Novel ◽  
Functional Nanomaterials ◽  
Complementary Sequences ◽  
Highly Sensitive ◽  
Target Molecules

Biosensors are very important for detecting target molecules with high accuracy, selectivity, and signal-to-noise ratio. Biosensors developed using biomolecules such as enzymes or nucleic acids which were used as the probes for detecting the target molecules were studied widely due to their advantages. For example, enzymes can react with certain molecules rapidly and selectively, and nucleic acids can bind to their complementary sequences delicately in nanoscale. In addition, biomolecules can be immobilized and conjugated with other materials by surface modification through the recombination or introduction of chemical linkers. However, these biosensors have some essential limitations because of instability and low signal strength derived from the detector biomolecules. Functional nanomaterials offer a solution to overcome these limitations of biomolecules by hybridization with or replacing the biomolecules. Functional nanomaterials can give advantages for developing biosensors including the increment of electrochemical signals, retention of activity of biomolecules for a long-term period, and extension of investigating tools by using its unique plasmonic and optical properties. Up to now, various nanomaterials were synthesized and reported, from widely used gold nanoparticles to novel nanomaterials that are either carbon-based or transition-metal dichalcogenide (TMD)-based. These nanomaterials were utilized either by themselves or by hybridization with other nanomaterials to develop highly sensitive biosensors. In this review, highly sensitive biosensors developed from excellent novel nanomaterials are discussed through a selective overview of recently reported researches. We also suggest creative breakthroughs for the development of next-generation biosensors using the novel nanomaterials for detecting harmful target molecules with high sensitivity.

Extremely low AFLP variation in the European dry rot fungus (Serpula lacrymans): implications for self/nonself-recognition

2004 ◽  
Vol 108 (11) ◽  
pp. 1264-1270 ◽  
Author(s):  
Håvard Kauserud ◽  
Olaf Schmidt ◽  
Malin Elfstrand ◽  
Nils Högberg
Keyword(s):  
Dry Rot ◽  
Nonself Recognition ◽  
Serpula Lacrymans

scholarly journals Nucleic Acids for Electrochemical Biosensor Technology

2020 ◽  
Author(s):  
Zihni Onur Uygun ◽  
Hilmiye Deniz Ertuğrul Uygun ◽  
Ferhan Girgin Sağin
Keyword(s):  
Electrical Properties ◽  
Nucleic Acids ◽  
New Technologies ◽  
Electrochemical Biosensor ◽  
Electrochemical Methods ◽  
Biological Molecules ◽  
End User ◽  
Biosensor Technology ◽  
One Step ◽  
Target Molecules

Biosensor technology has developed extremely rapidly in recent years. This technology brings along precise measurements as well as specific measurements. Thanks to its ability to be miniaturized and be easily accessible to the end user, it is one-step ahead of other similar methods. The selectivity of biological molecules and the sensitivity of electrochemical methods enable the continuous evolvement of these new technologies. In this chapter, the use of nucleic acids as both recognition agents and target molecules, the way they are used in biosensor technology and their electrical properties are explained in detail with examples. Aptamers, which are synthetic nucleic acids, and their use in electrochemical biosensor systems with different electrochemical and immobilization methods have been compared extensively.

scholarly journals Contrasting demographic histories revealed in two invasive populations of the dry rot fungus Serpula lacrymans

2021 ◽  
Author(s):  
Inger Skrede ◽  
Claude Murat ◽  
Jaqueline Hess ◽  
sundy Maurice ◽  
Jørn Henrik Sønstebø ◽  
...  
Keyword(s):  
Built Environment ◽  
Protein Modification ◽  
Intracellular Transport ◽  
Wood Decay ◽  
Local Environment ◽  
European Population ◽  
Indoor Environments ◽  
Dry Rot ◽  
Genomic Analyses ◽  
Serpula Lacrymans

Globalization and international trade have impacted organisms around the world leading to a considerable number of species establishing in new geographic areas. Many organisms have taken advantage of human-made environments, including buildings. One such species is the dry rot fungus Serpula lacrymans, which is the most aggressive wood-decay fungus in indoor environments in temperate regions. By using population genomic analyses of 36 full genome sequenced isolates, we revealed that isolates from Europe and Japan are highly divergent and that these populations split 3,000 - 19,000 generations ago, probably predating human influence. Approximately 250 generations ago, the European population went through a tight bottleneck, likely corresponding to the time it colonized the built environment. Moreover, evidence of admixture between European and Japanese populations was shown in an isolate from New Zealand. Genomic analyses revealed that low differentiation appeared in genes with functions related to of growth and intracellular transport, possibly important to its ability to effectively decay large substrates. These functions may have enabled both populations to independently establish in the human-made environment. Further, selective sweep analyses identified rapid changes in genes possibly related to decay of various substrates in Japan and in genes involved DNA replication and protein modification in Europe. These two fungal populations were preadapted to the built environment, but have more recently and independently adapted to their local environment.

Separation of Nucleic Acids Using Single- and Multimodal Chromatography

2018 ◽  
Vol 20 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Tiago Matos ◽  
Leif Bülow
Keyword(s):  
Nucleic Acids ◽  
Chemical Properties ◽  
Single Mode ◽  
Major Groove ◽  
Base Pairs ◽  
Chromatographic Separations ◽  
Multimodal Chromatography ◽  
Phosphate Backbone ◽  
Target Molecules ◽  
Polymerase Chain

The needs for purified nucleic acids for preparative and analytical applications have increased constantly, demanding for the development of new and more efficient methods for their recovery and isolation. DNA molecules harbour some intrinsic chemical properties that render them suitable for chromatographic separations. These include a negatively charged phosphate backbone as well as a hydrophobic character originating mainly from the major groove of DNA which exposes the base pairs on the surface of the molecule. In addition, single stranded DNA often allows for a free exposure of the hydrophobic aromatic bases. In this review, multimodal chromatography (MMC) has been evaluated as an alternative tool for complex separations of nucleic acids. MMC embraces more than one kind of interaction between the chromatographic ligand and the target molecules. These resins have often proved superior to conventional single-mode chromatographic materials for DNA isolation, including, e.g., the purification of plasmid DNA from crude cell lysates and for the preparation of DNA fragments before or after a polymerase chain reaction (PCR).

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