scholarly journals An Analysis of a Standard Reactor Cascade and a Step-Feed Reactor Cascade for Biological Processes Described by Monod Kinetics

2015 ◽  
Vol 10 (1) ◽  
pp. 27-37 ◽  
Author(s):  
Harvinder S. Sidhu ◽  
Mark Ian Nelson ◽  
Easwaran Balakrishnan
Keyword(s):  
Growth Rate ◽  
Steady State ◽  
Biological Treatment ◽  
Specific Growth ◽  
Biological Process ◽  
Equal Proportion ◽  
Biological Processes ◽  
Feed Stream ◽  
Monod Kinetics ◽  
Steady State Operation

Abstract We analyse the steady-state operation of two types of reactor cascade without recycle. The first is a standard reactor cascade in which the feed stream enters into the first reactor. The second is a step-feed reactor cascade in which an equal proportion of the feed stream enters each reactor in the cascade. The reaction is assumed to be a biological process governed by Monod growth kinetics with a decay coefficient for the microorganisms. The steady-states of both models are found for an arbitrary number of reactors and their stability determined as a function of the residence time. We show that in a step-feed reactor cascade the substrate and biomass concentrations leaving the reactor of the cascade are identical to those leaving the first reactor of the cascade. We further show that this result is true for a general specific growth rate of the form μ (S,X). Thus for such processes the non-standard cascade offers no advantage over that of a single reactor. This is surprising because the use of a non-standard cascade has been proposed as a mechanism to improve the biological treatment of wastewater.

Sebatian Metoksiaromatik Sebagai Substrat Pertumbuhan dan Pengaruh Enzim Demetilase dalam Acetobacterium Woodii DSM 1030

2012 ◽  
Author(s):  
Mohd. Sahaid Hj. Kalil ◽  
Muhammad Zaki ◽  
Wan Mohtar Wan Yusoff ◽  
Mohammad Ramlan Mohd. Salleh
Keyword(s):  
Growth Rate ◽  
Steady State ◽  
Sole Carbon Source ◽  
Specific Growth ◽  
Chemostat Culture ◽  
Carbon Sources ◽  
Syringic Acid ◽  
Organic Substrate ◽  
Acetobacterium Woodii ◽  
Vanilic Acid

Penyelidikan ini bertujuan untuk menyaring substrat organik bagi untuk penghasilan sel–sel A. woodii teraruh demetilase. Pertumbuhan A. woodii dilakukan dalam medium “Balch” yang mengandungi sumber karbon berbeza dalam keadaan anaerobik. Sebanyak sebelas substrat telah diuji iaitu anisol, 2– dan 3–metoksifenol, asid vanilik, asid siringik, asid 2,3,4–, 2,4,5– dan 3,4,5–trimetoksi benzoik, 2,3,4–, 2,4,5– dan 3,4,5–trimetoksi benzil alkohol. 2–metoksifenol merupakan substrat terbaik untuk pertumbuhan A. woodii pada kadar pertumbuhan spesifik 0.14 j–1. Penghasilan sel–sel teraruh demetilase dilakukan dalam kultur kemostat pada kadar pencairan (D) 0.0j–1. Sel-sel pada keadaan mantap dituai dalam keadaan anaerobik dan dipekatkan sebelum digunakan. Pertumbuhan A. woodii didapati maksimum dengan menggunakan kepekatan 0.62 g/L 2–metoksifenol sebagai sumber karbon tunggal. Tindak balas penyahmetilan oleh sel–sel A. woodii meningkat sebanyak 78% apabila 2–metoksifenol sebanyak 0.31 g/L ditambah dalam medium yang mengandungi fruktosa (1% w/v) semasa kultur kemostat. Kata kunci: tindak balas penyahmetilan; demetilase; sel-sel tertuai; metosiaromatik, Acetobacteriumwoodii The objective of this project was to screen organic substrate suitable for the growth of A. woodii, and as for the production of demethylase. A. woodii was grown in “Balch” medium containing different carbon sources. Eleven substrates were tested including anisole, 2– and 3–methoxyphenol, vanilic acid, syringic acid, 2,3,4–, 2,4,5– and 3,4,5–trimethoxy benzoic acid and 2,3,4–, 2,4,5– and 3,4,5–trimethoxy benzyl alcohol. It was found that 2–methoxyphenol was the best substrate with a specific growth rate of 0.14 h–1. The production of demethylase induced cells was carried out in a chemostat culture at a dilution rate (D) of 0.08 h–1. Cells were harvested at steady state of growth and concentrated before use. Optimal concentration of 2–methoxvphenol as the sole carbon source was 0.62 g/L. Demethylation reaction of 0.31 g/L 2–methoxyphenol by induced culture increases 78% relative to the chemostat culture containing only fructose. Key words: Demethylation reaction; demethylase; harvested cells; methoxyaromatic; Acetobacteriumwoodii

Biological Treatment Technology for Landfill Leachate

2015 ◽  
pp. 219-249
Author(s):  
Husnul Azan Bin Tajarudin ◽  
Mohd Firdaus Bin Othman ◽  
Noor Aziah Binti Serri ◽  
Muhammad Redzwan Bin Tamat
Keyword(s):  
Landfill Leachate ◽  
Biological Treatment ◽  
Biological Process ◽  
Low Cost ◽  
New Technology ◽  
Environmentally Friendly ◽  
Biological Processes ◽  
Treatment Technology ◽  
Leachate Treatment ◽  
Environmental Preservation

Biological process for environmental preservation and treatment is not a new technology. It was used a decade ago until now. The most important tools in biological processes are the microorganism and upstream instruments (bioreactor, pond and others) to run the process. Furthermore, the efficiency of the process depends on many factors such as temperature, pH, type of microorganism, conditions, and other nutrients. To understand the factors that will affect the process, mechanisms of microorganisms to treat or protect the environment must be considered. For leachate treatment, biological process is one of the most widely used techniques for low cost and environmentally friendly.

A BIOLOGICAL TREATMENT OF INDUSTRIAL WASTEWATERS: CONTOIS KINETICS

2015 ◽  
Vol 56 (4) ◽  
pp. 397-415 ◽  
Author(s):  
RUBAYYI T. ALQAHTANI ◽  
MARK I. NELSON ◽  
ANNETTE L. WORTHY
Keyword(s):  
Steady State ◽  
Residence Time ◽  
Continuous Flow ◽  
Biological Treatment ◽  
Membrane Bioreactor ◽  
Industrial Wastewaters ◽  
Steady State Operation ◽  
Recycle Ratio ◽  
Steady State Solutions ◽  
Bioreactor Performance

This paper analyses the steady-state operation of a generalized bioreactor model that encompasses a continuous-flow bioreactor and an idealized continuous-flow membrane bioreactor as limiting cases. A biodegradation of organic materials is modelled using Contois growth kinetics. The bioreactor performance is analysed by finding the steady-state solutions of the model and determining their stability as a function of the dimensionless residence time. We show that an effective recycle parameter improves the performance of the bioreactor at moderate values of the dimensionless residence time. However, at sufficiently large values of the dimensionless residence time, the performance of the bioreactor is independent of the recycle ratio.

Growth kinetics of Escherichia coli with galactose and several other sugars in carbon-limited chemostat culture

1999 ◽  
Vol 46 (1) ◽  
pp. 72-80 ◽  
Author(s):  
Urs Lendenmann ◽  
Mario Snozzi ◽  
Thomas Egli
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Steady State ◽  
Specific Growth Rate ◽  
Growth Kinetics ◽  
Specific Growth ◽  
Chemostat Culture ◽  
Monod Model ◽  
E Coli ◽  
Kinetics Of

Kinetic models for microbial growth describe the specific growth rate (μ) as a function of the concentration of the growth-limiting nutrient (s) and a set of parameters. A typical example is the model proposed by Monod, where μ is related to s using substrate affinity (Ks) and the maximum specific growth rate (μmax). The preferred method to determine such parameters is to grow microorganisms in continuous culture and to measure the concentration of the growth-limiting substrate as a function of the dilution rate. However, owing to the lack of analytical methods to quantify sugars in the microgram per litre range, it has not been possible to investigate the growth kinetics of Escherichia coli in chemostat culture. Using an HPLC method able to determine steady-state concentrations of reducing sugars, we previously have shown that the Monod model adequately describes glucose-limited growth of E. coli ML30. This has not been confirmed for any other sugar. Therefore, we carried out a similar study with galactose and found steady-state concentrations between 18 and 840 μg·L-1 for dilution rates between 0.2 and 0.8·h-1, respectively. With these data the parameters of several models giving the specific growth rate as a function of the substrate concentration were estimated by nonlinear parameter estimation, and subsequently, the models were evaluated statistically. From all equations tested, the Monod model described the data best. The parameters for galactose utilisation were μmax = 0.75·h-1 and Ks = 67 μg·L-1. The results indicated that accurate Ks values can be estimated from a limited set of steady-state data when employing μmax measured during balanced growth in batch culture. This simplified procedure was applied for maltose, ribose, and fructose. For growth of E. coli with these sugars, μmax and Ks were for maltose 0.87·h-1, 100 μg·L-1; for ribose 0.57·h-1, 132 μg·L-1, and for fructose 0.70·h-1, 125 μg·L-1. Key words: monod model, continuous culture, galactose, glucose, fructose, maltose, ribose.

scholarly journals Analysis of Gene Expression in Escherichia coli in Response to Changes of Growth-Limiting Nutrient in Chemostat Cultures

2004 ◽  
Vol 70 (4) ◽  
pp. 2354-2366 ◽  
Author(s):  
Qiang Hua ◽  
Chen Yang ◽  
Taku Oshima ◽  
Hirotada Mori ◽  
Kazuyuki Shimizu
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Growth Rate ◽  
Steady State ◽  
Specific Growth ◽  
Differentially Expressed ◽  
Transcriptional Responses ◽  
Chemostat Cultures ◽  
Limiting Nutrient ◽  
Slow Growing

ABSTRACT Studies of steady-state metabolic fluxes in Escherichia coli grown in nutrient-limited chemostat cultures suggest remarkable flux alterations in response to changes of growth-limiting nutrient in the medium (Hua et al., J. Bacteriol. 185:7053-7067, 2003). To elucidate the physiological adaptation of cells to the nutrient condition through the flux change and understand the molecular mechanisms underlying the change in the flux, information on gene expression is of great importance. DNA microarray analysis was performed to investigate the global transcriptional responses of steady-state cells grown in chemostat cultures with limited glucose or ammonia while other environmental conditions and the growth rate were kept constant. In slow-growing cells (specific growth rate of 0.10 h−1), 9.8% of a total of 4,071 genes investigated, especially those involved in amino acid metabolism, central carbon and energy metabolism, transport system and cell envelope, were observed to be differentially expressed between the two nutrient-limited cultures. One important characteristic of E. coli grown under nutrient limitation was its capacity to scavenge carbon or nitrogen from the medium through elevating the expression of the corresponding transport and assimilation genes. The number of differentially expressed genes in faster-growing cells (specific growth rate of 0.55 h−1), however, decreased to below half of that in slow-growing cells, which could be explained by diverse transcriptional responses to the growth rate under different nutrient limitations. Independent of the growth rate, 92 genes were identified as being differentially expressed. Genes tightly related to the culture conditions were highlighted, some of which may be used to characterize nutrient-limited growth.

Biological Treatment Technology for Landfill Leachate

2020 ◽  
pp. 775-806
Author(s):  
Husnul Azan Bin Tajarudin ◽  
Mohd Firdaus Bin Othman ◽  
Noor Aziah Binti Serri ◽  
Muhammad Redzwan Bin Tamat
Keyword(s):  
Landfill Leachate ◽  
Biological Treatment ◽  
Biological Process ◽  
Low Cost ◽  
New Technology ◽  
Environmentally Friendly ◽  
Biological Processes ◽  
Treatment Technology ◽  
Leachate Treatment ◽  
Environmental Preservation

Biological process for environmental preservation and treatment is not a new technology. It was used a decade ago until now. The most important tools in biological processes are the microorganism and upstream instruments (bioreactor, pond and others) to run the process. Furthermore, the efficiency of the process depends on many factors such as temperature, pH, type of microorganism, conditions, and other nutrients. To understand the factors that will affect the process, mechanisms of microorganisms to treat or protect the environment must be considered. For leachate treatment, biological process is one of the most widely used techniques for low cost and environmentally friendly.

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