Trefoil factor family peptides are increased in the saliva of children with mucositis

2011 ◽  
Vol 49 (12) ◽  
Author(s):  
Fran Verey ◽  
Ebba Nexo ◽  
Rosemary Greenwood ◽  
Monica Berry ◽  
Anthony P. Corfield
Keyword(s):  
Rank Correlation ◽  
Oral Disease ◽  
Healthy Children ◽  
Trefoil Factor ◽  
Oncology Patients ◽  
Trefoil Peptides ◽  
Trefoil Peptide ◽  
Healing Phase ◽  
Trefoil Factor Family ◽  
Pre Treatment

AbstractMucositis is a painful ulcerative condition of the oral cavity and gastrointestinal tract, occurring in association with chemotherapy and radiotherapy regimes. Trefoil factor family peptides (TFF, trefoil peptides), present in saliva, contribute to epithelial restitution and repair and are therefore potentially important in the healing phase of mucositis. This study aimed to assess any changes in the levels of trefoil peptides in oncology patients with and without mucositis.Saliva was collected from healthy children, pre-treatment oncology patients, neutropenic patients on treatment with no oral disease and mucositic patients. TFF1, 2 and 3 were quantified using ELISA.In healthy children TFF2 and 3 were positively correlated with age (ρ=0.454, p=0.01 for TFF2; ρ=0.410, p=0.05 for TFF3 Spearman rank correlation). TFF3 was higher in mucositis compared to all other groups. A linear regression prediction model indicated that TFF3, but not TFF1 and TFF2, was significantly different in mucositic and healthy controls, suggesting an altered pattern of trefoil peptide secretion (p=0.021).This study is the first to focus on trefoil peptides in paediatric saliva. It shows the correlation between TFF2, TFF3 and age in healthy children. Paediatric mucositis disease occurs in the presence of increased concentrations and an altered pattern of trefoil peptides.

Validation of commercial assays for measurements of trefoil factor family peptides in serum

2011 ◽  
Vol 49 (12) ◽  
Author(s):  
Mie H. Samson ◽  
Ebba Nexo
Keyword(s):  
Blood Donors ◽  
Reference Intervals ◽  
Cross Reactivity ◽  
Serum Concentrations ◽  
Trefoil Factor ◽  
Serum Samples ◽  
Measuring Range ◽  
Trefoil Peptides ◽  
Pathological Conditions ◽  
Trefoil Factor Family

AbstractTrefoil peptides (TFF1, TFF2 and TFF3) are 7–12 kDa molecules, secreted by mucin-producing epithelial cells. Increased serum concentrations have been reported in a number of pathological conditions, which warrants the need for validated commercially available assays.We validated commercial assays for TFF1-3 and compared results obtained with our in-house assays, using serum from blood donors.Level of detection was: ≤0.008 nmol/L. Measuring ranges were: 0.032–0.51 (TFF1), 0.038–0.76 (TFF2) and 0.019–0.15 (TFF3) nmol/L. Imprecision (CV), judged from the measurement of serum pools in two levels, was below 9% (TFF2 and TFF3) but up to 18% (mean 0.41 nmol/L) for TFF1. No cross reactivity between the TFFs (concentrations >100 nmol/L) was observed. The 95% non-parametric reference intervals were: <0.0032–0.53 (TFF1), 0.099–1.4 (TFF2) and 0.086–0.87 (TFF3) nmol/L. Comparing commercial to in-house assays (n=132), showed biases explained by differences in the calibrators (TFF1 and TFF2). A number of samples showed markedly different results.The commercial assays for TFF2 and TFF3 are acceptable for use on serum samples, while the TFF1 assay revealed a poor imprecision and a too narrow measuring range. Results obtained with the commercial and the in-house assays differed, partly because of differences in the calibrators employed.

scholarly journals Production and comparison of mature single-domain ‘trefoil’ peptides pNR-2/pS2 Cys58 and pNR-2/pS2 Ser58

1995 ◽  
Vol 308 (3) ◽  
pp. 1001-1007 ◽  
Author(s):  
M P Chadwick ◽  
F E B May ◽  
B R Westley
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Gel Filtration ◽  
Thiol Group ◽  
Cysteine Residue ◽  
Exchange Chromatography ◽  
Single Domain ◽  
Trefoil Peptides ◽  
Trefoil Peptide

The preparation and purification of recombinant mature pNR-2/pS2, a single-domain member of the ‘trefoil’ family of cysteine-rich secreted proteins, is described. Analysis of recombinant pNR-2/pS2 by ion-exchange chromatography showed that it was heterogeneous. The heterogeneity was reduced by treatment with thiol-group-containing reagents, suggesting that it is caused by the odd number of cysteine residues in mature pNR-2/pS2, and this view was reinforced by mutation of the extra-trefoil domain cysteine residue, Cys58, to a serine residue. Electrophoresis of recombinant pNR-2/pS2 Cys58 and pNR-2/pS2 Ser58 proteins under non-denaturing conditions confirmed that the Ser58 mutant is much more homogeneous, and showed that most of pNR-2/pS2 Ser58 co-migrates as a single band with pNR-2/pS2 secreted from breast-cancer cells in culture. Treatment of recombinant pNR-2/pS2 proteins with various thiol-group-reactive reagents indicated that cysteine is the most effective at producing recombinant pNR-2/pS2 that co-migrates with pNR-2/pS2 secreted by breast-cancer cells. Dithiothreitol appeared to denature the proteins, and GSH was relatively ineffective. pNR-2/pS2 Cys58 treated with cysteine and untreated pNR-2/pS2 Ser58 had the same apparent molecular mass, measured by gel filtration, as pNR-2/pS2 secreted from breast-cancer cells. This is the first report of the production of a recombinant mature single-domain trefoil peptide and should greatly facilitate elucidation of the structure and function of pNR-2/pS2.

Trefoil factor family peptide 2 acts pro-proliferative and pro-apoptotic in the murine retina

2011 ◽  
Vol 135 (5) ◽  
pp. 461-473 ◽  
Author(s):  
Adnana N. Paunel-Görgülü ◽  
Andreas G. Franke ◽  
Friedrich P. Paulsen ◽  
Nicole Dünker
Keyword(s):  
Trefoil Factor ◽  
Trefoil Factor Family

Serum and Saliva 25(OH)D Levels in Relation to Dental Caries in Young Children

2021 ◽  
Vol 45 (6) ◽  
pp. 414-420
Author(s):  
Alaa Sabah Hussein ◽  
Manal Mohamed Almoudi ◽  
Mohamed Ibrahim Abu-Hassan ◽  
Robert J Schroth ◽  
Bahruddin Saripudin ◽  
...  
Keyword(s):  
Dental Caries ◽  
Young Children ◽  
Rank Correlation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Children ◽  
Spearman’S Rank Correlation ◽  
Increased Risk ◽  
Whole Saliva ◽  
Dmft Index ◽  
Unstimulated Whole Saliva

Objective: Several studies have reported that low levels of vitamin D (25(OH)D) are associated with an increased risk of dental caries and that optimal levels may offer protection This study aimed to assess the relationship between serum and saliva 25(OH)D levels and caries among young children. Study design: A total of 120 healthy children were recruited; 93 with caries and 27 caries-free. Dental caries status was evaluated using decayed, missing and filled in primary teeth (dmft) index. Blood and unstimulated whole saliva samples were collected. Laboratory analysis was performed using Enzyme-Linked Immunosorbent Assay Kit. Data were analyzed with descriptive statistics, bivariate and Spearman’s rank correlation analysis. Results: There were no significant associations between serum and saliva 25(OH)D levels and caries status (P &gt; 0.05). Levels of 25(OH)D in serum were significantly higher than levels found in saliva (P &lt; 0.05), and a correlation between serum and saliva 25(OH)D levels was observed (P &lt; 0.05). Conclusions: The association between serum and saliva 25(OH)D and dental caries in young children was inconclusive. However, a positive and significant correlation was observed between serum and saliva 25(OH)D levels. Further studies are warranted to investigate the definite relation between 25(OH)D levels and dental caries and using saliva 25(OH)D as a non-invasive alternative method over blood samples.

scholarly journals Expression of Trefoil Factor Family Members Correlates with Patient Prognosis and Neoangiogenesis

2005 ◽  
Vol 11 (18) ◽  
pp. 6472-6478 ◽  
Author(s):  
Dipok Kumar Dhar ◽  
Timothy C. Wang ◽  
Hideki Tabara ◽  
Yasuhito Tonomoto ◽  
Riruke Maruyama ◽  
...  
Keyword(s):  
Family Members ◽  
Trefoil Factor ◽  
Trefoil Factor Family ◽  
Patient Prognosis

Alterations of Serum Osteocalcin Levels in Patients with Legg-Calvé-Perthes

2016 ◽  
Vol 27 (1) ◽  
pp. 92-95
Author(s):  
Wen-Bo Wei ◽  
Sha-Jie Dang ◽  
Yong Li ◽  
Zong-Zhi Liu
Keyword(s):  
Femoral Head ◽  
Healthy Volunteers ◽  
Rank Correlation ◽  
Serum Levels ◽  
Additional Treatment ◽  
Rank Test ◽  
Healthy Children ◽  
Serum Osteocalcin ◽  
Signed Rank ◽  
Signed Rank Test

Background Legg-Calvé-Perthes disease (LCPD) is a paediatric form of osteonecrosis that ultimately heals but will cause femoral head and acetabular deformities. The purpose of this study was to investigate the early serum measurement of osteocalcin in children with femoral head necrosis compared with that of healthy children. Methods 20 patients with LCPD (4 girls and 16 boys) and 20 healthy volunteers (5 girls and 15 boys) were enrolled. All patients did not have additional treatment. Blood samples were obtained from all patients pre-operatively in the morning. All cases had hip radiographs. The serum levels of osteocalcin comparisons between the LCPD patients and the healthy volunteers were performed using Wilcoxon signed-rank test. The Spearman rank correlation was used to assess correlation between LCPD grade and serum osteocalcin levels. Significance was set at p = 0.05. Results The 20 patients with LCPD (72.75 ± 24.92 ng/ml) had significantly higher serum osteocalcin levels compared with the healthy group (16.80 ± 4.04 ng/ml) (p≤0.01). Serum osteocalcin levels of different LCPD grades were significantly different (Spearman's p = 0.540, p = 0.014). Conclusions We observed a significant correlation between serum osteocalcin levels and LCPD. These results may be meaningful in clinical practice and to future studies.

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