scholarly journals Production and comparison of mature single-domain ‘trefoil’ peptides pNR-2/pS2 Cys58 and pNR-2/pS2 Ser58

1995 ◽  
Vol 308 (3) ◽  
pp. 1001-1007 ◽  
Author(s):  
M P Chadwick ◽  
F E B May ◽  
B R Westley
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Gel Filtration ◽  
Thiol Group ◽  
Cysteine Residue ◽  
Exchange Chromatography ◽  
Single Domain ◽  
Trefoil Peptides ◽  
Trefoil Peptide

The preparation and purification of recombinant mature pNR-2/pS2, a single-domain member of the ‘trefoil’ family of cysteine-rich secreted proteins, is described. Analysis of recombinant pNR-2/pS2 by ion-exchange chromatography showed that it was heterogeneous. The heterogeneity was reduced by treatment with thiol-group-containing reagents, suggesting that it is caused by the odd number of cysteine residues in mature pNR-2/pS2, and this view was reinforced by mutation of the extra-trefoil domain cysteine residue, Cys58, to a serine residue. Electrophoresis of recombinant pNR-2/pS2 Cys58 and pNR-2/pS2 Ser58 proteins under non-denaturing conditions confirmed that the Ser58 mutant is much more homogeneous, and showed that most of pNR-2/pS2 Ser58 co-migrates as a single band with pNR-2/pS2 secreted from breast-cancer cells in culture. Treatment of recombinant pNR-2/pS2 proteins with various thiol-group-reactive reagents indicated that cysteine is the most effective at producing recombinant pNR-2/pS2 that co-migrates with pNR-2/pS2 secreted by breast-cancer cells. Dithiothreitol appeared to denature the proteins, and GSH was relatively ineffective. pNR-2/pS2 Cys58 treated with cysteine and untreated pNR-2/pS2 Ser58 had the same apparent molecular mass, measured by gel filtration, as pNR-2/pS2 secreted from breast-cancer cells. This is the first report of the production of a recombinant mature single-domain trefoil peptide and should greatly facilitate elucidation of the structure and function of pNR-2/pS2.

scholarly journals Betavulgarin Isolated from Sugar Beet (Beta vulgaris) Suppresses Breast Cancer Stem Cells through Stat3 Signaling

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 2999
Author(s):  
Ren Liu ◽  
Hack Sun Choi ◽  
Xing Zhen ◽  
Su-Lim Kim ◽  
Ji-Hyang Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Gel Filtration ◽  
Resonance Spectroscopy ◽  
Breast Cancer Stem Cells ◽  
Self Renewal ◽  
Major Health Problem

Breast cancer is a major health problem that affects lives worldwide. Breast cancer stem cells (BCSCs) are small subpopulations of cells with capacities for drug resistance, self-renewal, recurrence, metastasis, and differentiation. Herein, powder extracts of beetroot were subjected to silica gel, gel filtration, thin layer chromatography (TLC), and preparatory high-pressure liquid chromatography (HPLC) for isolation of one compound, based on activity-guided purification using tumorsphere formation assays. The purified compound was identified as betavulgarin, using nuclear magnetic resonance spectroscopy and electrospray ionization (ESI) mass spectrometry. Betavulgarin suppressed the proliferation, migration, colony formation, and mammosphere formation of breast cancer cells and reduced the size of the CD44+/CD24− subpopulation and the expression of the self-renewal-related genes, C-Myc, Nanog, and Oct4. This compound decreased the total level and phosphorylated nuclear level of signal transducer and activator of transcription 3 (Stat3) and reduced the mRNA and protein levels of sex determining region Y (SRY)-box 2 (SOX2), in mammospheres. These data suggest that betavulgarin inhibit the Stat3/Sox2 signaling pathway and induces BCSC death, indicating betavulgarin might be an anticancer agent against breast cancer cells and BCSCs.

scholarly journals A Therapeutic System of 177Lu-labeled Gold Nanoparticles-RGD Internalized in Breast Cancer Cells

2017 ◽  
Vol 57 (3) ◽  
Author(s):  
Myrna Luna-Gutiérrez ◽  
Guillermina Ferro-Flores ◽  
Blanca E. Ocampo-García ◽  
Clara L. Santos- Cuevas ◽  
Nallely Jiménez-Mancilla ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Photothermal Therapy ◽  
Breast Cancer Cells ◽  
Mcf7 Cell ◽  
Thiol Group ◽  
20 Nm ◽  
Spontaneous Reaction

The aim of this research was to evaluate the <em>in vitro</em> potential of <sup>177</sup>Lu-labeled gold nanoparticles conjugated to cyclo-[RGDfK(C)] peptides (<sup>177</sup>Lu-AuNP-c[RGDfK(C)]) as a plasmonic photothermal therapy and targeted radiotherapy system in MCF7 breast cancer cells. Peptides were conjugated to AuNPs (20 nm) by spontaneous reaction with the thiol group of cysteine (C). After laser irradiation, the presence of c[RGDfK(C)]-AuNP in cells caused a significant increase in the temperature of the medium (50.5 °C, compared to 40.3 °C without AuNPs) resulting in a significant decrease in MCF7 cell viability down to 9 %. After treatment with <sup>177</sup>-AuNP-c[RGDfK(C)], the MCF7 cell proliferation was significantly inhibited.

Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells

ACS Nano ◽  
2014 ◽  
Vol 8 (6) ◽  
pp. 5682-5695 ◽  
Author(s):  
Tatsiana Y. Rakovich ◽  
Omar K. Mahfoud ◽  
Bashir M. Mohamed ◽  
Adriele Prina-Mello ◽  
Kieran Crosbie-Staunton ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Quantum Dot ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Highly Sensitive

The proliferation of breast cancer cells are inhibited by the human intrabody targeting cyclinD1

2010 ◽  
Vol 34 (8) ◽  
pp. S49-S49
Author(s):  
Lei Wang ◽  
Xun Zhou ◽  
Lihong Zhou ◽  
Yong Chen ◽  
Xun Zhu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells

Asperjinone, a norneolignan, and terrein, a suppressor of ABCG2-expressing breast cancer cells, from Aspergillus terreus

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
WY Liao ◽  
CN Shen ◽  
LH Lin ◽  
YL Yang ◽  
HY Han ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Aspergillus Terreus
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