Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates

2010 ◽  
Vol 53 (3) ◽  
Author(s):  
Chinnarjan Ravindran ◽  
Thangiah Naveenan ◽  
Govindaswamy R. Varatharajan
Keyword(s):  
Cellulase Production ◽  
Industrial Wastes ◽  
Alkaline Cellulase

scholarly journals Production of cellulase by Mucor ramanniacus using submerged fermentation and its applications in biodegradation of agro-industrial waste

2021 ◽  
Author(s):  
Taiwo Dorcas Ibukunoluwa ◽  
Ademakinwa Adedeji Nelson ◽  
Zainab Adenike Ayinla ◽  
Femi Kayode Agboola
Keyword(s):  
Specific Activity ◽  
Protein A ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Growth Conditions ◽  
Cellulase Production ◽  
Industrial Applications ◽  
Inoculum Size ◽  
Industrial Wastes ◽  
Triton X

Abstract This study was undertaken to isolate and identify a novel cellulase-producing strain from a waste site (7°28’11’’N 4°31’24’’E), optimise the growth conditions, partially purify and biochemically characterise the enzyme. The potentials of the purified cellulase to hydrolyse the lignocellulosic component of some agro-industrial wastes (e.g. orange peels etc.) was also investigated. The best cellulase-producing fungus was identified as Mucor ramanniacus and the optimum conditions for cellulase production were pH (4.5), inoculum size (12 mm), carbon and nitrogen sources were carboxymethyl cellulose and sodium nitrate respectively resulting in a specific activity of 1423 Units/mg protein. A purification fold of 1.56 and 45.37 % yield were obtained after purification. The optimum pH and temperature were at 9.0 and 40°C respectively. The kinetic parameters were 0.63 ± 0.495 mg/ml, 20.21 ± 11.28 U/ml, 1001.4s− 1 for Km and Vmax and kcat respectively. Na+, K+, Ca+, Cysteine, β-mercaptoethanol and SDS were activators while Tween 80, Triton X-100 EDTA, Hg2+ and Ba2+ inhibited the enzyme. M. ramanniacus cellulase hydrolysed all agro-industrial wastes used. The partially purified M. ramanniacus cellulase showed great potential in biodegradation of various lignocellulosic substrates and the biochemical characteristics exhibited makes it suitable in industrial applications.

Alkaline Cellulase Production by Penicillium mallochii LMB-HP37 Isolated from Soils of a Peruvian Rainforest

2015 ◽  
Vol 7 (4) ◽  
pp. 160-168 ◽  
Author(s):  
Karin Vega ◽  
Victor Sarmiento ◽  
Yvette Ludeña ◽  
Nadia Vera ◽  
Carmen Tamariz-Angeles ◽  
...  
Keyword(s):  
Cellulase Production ◽  
Alkaline Cellulase

scholarly journals Production of Alkaline Cellulase by Fungi Isolated from an Undisturbed Rain Forest of Peru

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Karin Vega ◽  
Gretty K. Villena ◽  
Victor H. Sarmiento ◽  
Yvette Ludeña ◽  
Nadia Vera ◽  
...  
Keyword(s):  
Rain Forest ◽  
Textile Industry ◽  
Cellulase Production ◽  
Selective Medium ◽  
Ph Values ◽  
Biological Interest ◽  
Apparent Decrease ◽  
Alkaline Cellulase ◽  
Dilution Plate Method ◽  
Soil Dilution

Alkaline cellulase producing fungi were isolated from soils of an undisturbed rain forest of Peru. The soil dilution plate method was used for the enumeration and isolation of fast growing cellulolytic fungi on an enriched selective medium. Eleven out of 50 different morphological colonies were finally selected by using the plate clearing assay with CMC as substrate at different pH values. All 11 strains produced cellulases in liquid culture with activities at alkaline pH values without an apparent decrease of them indicating that they are true alkaline cellulase producers. Aspergillus sp. LM-HP32, Penicillium sp. LM-HP33, and Penicillium sp. LM-HP37 were the best producers of FP cellulase (>3 U mL−1) with higher specific productivities (>30 U g−1 h−1). Three strains have been found suitable for developing processes for alkaline cellulase production. Soils from Amazonian rain forests are good sources of industrial fungi with particular characteristics. The results of the present study are of commercial and biological interest. Alkaline cellulases may be used in the polishing and washing of denim processing of the textile industry.

Preliminary results of the optimization of biogas production at the biogas station of the national research institute of animal production in Grodziec Sląski – Kostkowice

2015 ◽  
Vol 2 (3) ◽  
pp. 26-31
Author(s):  
K. Węglarzy ◽  
Yu. Shliva ◽  
B. Matros ◽  
G. Sych
Keyword(s):  
Agricultural Production ◽  
Crude Protein ◽  
Biogas Production ◽  
Electric Energy ◽  
Corn Silage ◽  
Industrial Wastes ◽  
Organic Mass ◽  
National Research Institute ◽  
Digestion Process ◽  
By Products

Aim. To optimize the methane digestion process while using different recipes of substrate components of ag- ricultural origin. Methods. The chemical composition of separate components of the substrate of agricultural by-products, industrial wastes, fats of the agrorefi nery and corn silage was studied. Dry (organic) mass, crude protein (fat) fi ber, loose ash, nitrogen-free exhaust were estimated in the components and the productivity of biogas was determined along with the methane content. These data were used as a basis for daily recipes of the substrate and the analysis of biogas production at the biogas station in Kostkowice. Results. The application of by-products of agricultural production solves the problem of their storage on boards and in open containers, which reduces investment costs, related to the installation of units for their storage. Conclusions. The return on investment for obtaining electric energy out of agricultural biogas depends considerably on the kind of the substrate used and on technological and market conditions.

Cellulase production and morphology of Trichoderma reesei in different experimental conditions

2018 ◽  
Vol 63 (2) ◽  
pp. 115-129
Author(s):  
Rahela Carpa ◽  
◽  
Alin Cândea ◽  
Alexei Remizovschi ◽  
Lucian Barbu-Tudoran ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Experimental Conditions

Review on Analysis of Stir Cast Aluminium Metal Matrix Composite from Agro-Industrial Wastes

2017 ◽  
Vol 5 (2) ◽  
pp. 35 ◽  
Author(s):  
CHANDLA NAGENDER KUMAR ◽  
YASHPAL ◽  
JAWALKAR C. S. ◽  
SURI N. M ◽  
◽  
...  
Keyword(s):  
Metal Matrix Composite ◽  
Matrix Composite ◽  
Metal Matrix ◽  
Industrial Wastes ◽  
Cast Aluminium ◽  
Aluminium Metal

VALORIZATION OF AGRO-INDUSTRIAL WASTES IN LIGHTWEIGHT AGGREGATES FOR AGRONOMIC USE: PRELIMINARY STUDY

2017 ◽  
Vol 16 (8) ◽  
pp. 1691-1699 ◽  
Author(s):  
Romina D. Farias ◽  
Carmen Martinez Garcia ◽  
Teresa Cotes Palomino ◽  
Fernanda Andreola ◽  
Isabella Lancellotti ◽  
...  
Keyword(s):  
Industrial Wastes ◽  
Lightweight Aggregates ◽  
Preliminary Study

NEW VIABLE INDUSTRIAL WASTES MIX FOR FODDER YEASTS PRODUCTION

2014 ◽  
Vol 13 (7) ◽  
pp. 1611-1621
Author(s):  
Andrei I. Simion ◽  
Livia Manea ◽  
Cristina G. Grigoras ◽  
Lidia Favier-Teodorescu
Keyword(s):  
Industrial Wastes
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