Involvement of actin and microtubules in regulation of bioluminescence and translocation of chloroplasts in the dinoflagellate Pyrocystis lunula

2009 ◽  
Vol 52 (2) ◽  
Author(s):  
Kirsten Heimann ◽  
Paul L. Klerks ◽  
Karl H. Hasenstein
Keyword(s):  
Pyrocystis Lunula

Construction of cDNA library of Pyrocystis lunula (Pyrophyta)

2004 ◽  
Vol 3 (2) ◽  
pp. 141-144
Author(s):  
Zhenghong Sui ◽  
Klaus V. Kowallik
Keyword(s):  
Cdna Library ◽  
Pyrocystis Lunula

Cloning and Characterization of an Active Fragment of Luciferase from a Luminescent Marine Alga, Pyrocystis lunula¶

2007 ◽  
Vol 75 (3) ◽  
pp. 311-315
Author(s):  
Hisashi Morishita ◽  
Sayumi Ohashi ◽  
Takashi Oku ◽  
Yoshihiro Nakajima ◽  
Satoshi Kojima ◽  
...  
Keyword(s):  
Marine Alga ◽  
Active Fragment ◽  
Pyrocystis Lunula

scholarly journals An ”omic“ approach to Pyrocystis lunula: New insights related with this bioluminescent dinoflagellate

2019 ◽  
Author(s):  
Carlos Fajardo ◽  
Francisco Amil-Ruiz ◽  
Carlos Fuentes Almagro ◽  
Marcos De Donato ◽  
Almudena Escobar Niño ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Protein Identification ◽  
Gene Expression Regulation ◽  
Molecular Level ◽  
Gene Ontology Classification ◽  
The Trinity ◽  
Very High ◽  
Shed Light ◽  
Comprehensive Reference ◽  
Pyrocystis Lunula

Abstract Background: Pyrocystis lunula (Schutt) is a photoautotrophic dinoflagellate without armored, frequently found in marine environments. Today, there are several biotechnological applications derived from the bioluminescent system of this species. From a post-genomic perspective, in order to study the whole proteome of P. lunula, an ”omic“ approach (transcriptomic-proteomic analysis) was assessed using fresh microalgae samples. Results: A total of 80,874,825 raw reads were generated (11,292,087,505 pb; 55.82 % GC) by mRNA sequencing. Very high quality sequences were assembled into 414,295 contigs (219,203,407 pb; 55.38 % GC) by the Trinity software, generating a comprehensive reference transcriptome for this species. Then, a P. lunula proteome was predicted and further employed through the first proteomic analysis on this species. A total of 17,461 peptides were identified, yielding 3,182 protein identification hits. The identified proteins were further categorized according to functional description and gene ontology classification. Conclusions: The first comprehensive molecular analysis of the microalgae P. lunula using both, transcriptomic and proteomic approaches, has been reported. Proteomic results represent a valuable piece in the understanding of this microalgae regulation at molecular level, and shed light on the identification of important factors involved in gene expression regulation. Indeed, the presence of the luciferin-binding protein (LBP), which had not been described so far in the genus Pyrocystis has been highlighted.

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