Isolation, fatty acid profiles and cryopreservation of marine thraustochytrids from mangrove habitats in Thailand

2017 ◽  
Vol 60 (4) ◽  
Author(s):  
Panida Unagul ◽  
Satinee Suetrong ◽  
Sita Preedanon ◽  
Anupong Klaysuban ◽  
Wunna Gundool ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Molecular Phylogeny ◽  
Polyunsaturated Fatty Acids ◽  
Total Fatty Acid ◽  
Fatty Acid Profiles ◽  
Alternative Source ◽  
Marine Protists ◽  
Potential Use ◽  
High Diversity

AbstractThraustochytrids, marine protists, have attracted attention as a proven alternative source of polyunsaturated fatty acids (PUFAs). We isolated a high diversity of thraustochytrids from Thailand and tested their potential use in the production of high-value fatty acids. The isolated thraustochytrids can be categorized into seven major groups based on unique morphological features, molecular phylogeny and fatty acid profiles. Two of the seven isolated thraustochytrid groups could potentially be new lineages in the Labyrinthulomycetes. The production of total fatty acid (TFA) from these thraustochytrids ranged from 2.4 to 35.6% (w/w), with biomass varying from 0.3 to 8.9 g l

scholarly journals Fatty acid profiles of muscle, liver, heart and kidney of Australian prime lambs fed different polyunsaturated fatty acids enriched pellets in a feedlot system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hung Van Le ◽  
Don Viet Nguyen ◽  
Quang Vu Nguyen ◽  
Bunmi Sherifat Malau-Aduli ◽  
Peter David Nichols ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Profiles

scholarly journals Nutritional Enhancement of Health Beneficial Omega-3 Long-Chain Polyunsaturated Fatty Acids in the Muscle, Liver, Kidney, and Heart of Tattykeel Australian White MARGRA Lambs Fed Pellets Fortified with Omega-3 Oil in a Feedlot System

Biology ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 912
Author(s):  
Shedrach Benjamin Pewan ◽  
John Roger Otto ◽  
Robert Tumwesigye Kinobe ◽  
Oyelola Abdulwasiu Adegboye ◽  
Aduli Enoch Othniel Malau-Aduli
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Human Health ◽  
Chain Polyunsaturated Fatty Acid ◽  
Fatty Acid Profiles ◽  
Omega 3 ◽  
Pufa Composition ◽  
Nutritional Enhancement ◽  
Long Chain

The aim of this research was to evaluate the nutritional enhancement of omega-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA) composition of edible lamb Longissimus thoracis et lumborum muscle, heart, kidney, and liver in response to dietary supplementation of lot-fed lambs with or without omega-3 oil fortified pellets. The hypothesis tested was that fortifying feedlot pellets with omega-3 oil will enhance the human health beneficial n-3 LC-PUFA composition of edible lamb muscle tissue and organs. Seventy-five Tattykeel Australian White lambs exclusive to the MARGRA brand, with an average body weight of 30 kg at six months of age, were randomly assigned to the following three dietary treatments of 25 lambs each, and lot-fed as a cohort for 47 days in a completely randomized experimental design: (1) Control grain pellets without oil plus hay; (2) Omega-3 oil fortified grain pellets plus hay; and (3) Commercial whole grain pellets plus hay. All lambs had ad libitum access to the basal hay diet and water. Post-slaughter fatty acid composition of the Longissimus thoracis et lumborum muscle, liver, kidney, and heart were determined using thee gas chromatography–mass spectrophotometry technique. Results indicated significant variations (p < 0.05) in fatty acid profiles between tissues and organs. Omega-3 oil fortified pellets significantly (p < 0.05) increased ≥C20 n-3 LC-PUFA (C20:5n-3 eicosapentaenoate, EPA + C22:5n3 docosapentaenoate, DPA + C22:6n3 docosahexanoate DHA); C18:3n-3 alpha-linolenate, ALA; C18:2 conjugated linoleic acid, CLA; total monounsaturated fatty acids, MUFA; polyunsaturated fatty acids, PUFA contents; and reduced the ratio of omega-6 to omega-3 fatty acids in all lamb organs and tissues without impacting shelf-life. The findings demonstrate that the inclusion of omega-3 oil in feedlot diets of lambs enhances the human health beneficial omega-3 long-chain polyunsaturated fatty acid profiles of edible muscle tissue and organs without compromising meat quality.

scholarly journals Fatty Acid Profiles of Stipe and Blade from the Norwegian Brown Macroalgae Laminaria hyperborea with Special Reference to Acyl Glycerides, Polar Lipids, and Free Fatty Acids

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Lena Foseid ◽  
Hanne Devle ◽  
Yngve Stenstrøm ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Free Fatty Acids ◽  
Neutral Lipids ◽  
Dry Weight ◽  
Polar Lipids ◽  
Fatty Acid Profiles ◽  
Laminaria Hyperborea ◽  
Lipid Fractions

A thorough analysis of the fatty acid profiles of stipe and blade from the kelp species Laminaria hyperborea is presented. Lipid extracts were fractionated into neutral lipids, free fatty acids, and polar lipids, prior to derivatization and GC-MS analysis. A total of 42 fatty acids were identified and quantified, including the n-3 fatty acids α-linolenic acid, stearidonic acid, and eicosapentaenoic acid. The fatty acid amounts are higher in blade than in stipe (7.42 mg/g dry weight and 2.57 mg/g dry weight, resp.). The highest amounts of n-3 fatty acids are found within the neutral lipid fractions with 590.6 ug/g dry weight and 100.9 ug/g dry weight for blade and stipe, respectively. The amounts of polyunsaturated fatty acids are 3.4 times higher in blade than stipe. The blade had the highest PUFA/SFA ratio compared to stipe (1.02 versus 0.76) and the lowest n-6/n-3 ratio (0.8 versus 3.5). This study highlights the compositional differences between the lipid fractions of stipe and blade from L. hyperborea. The amount of polyunsaturated fatty acids compared to saturated- and monounsaturated fatty acids is known to influence human health. In the pharmaceutical, food, and feed industries, this can be of importance for production of different health products.

scholarly journals Utilization of Schizochytrium mangrovei PQ6 as feed for rotifer Brachionus plicatilis in rearing black sleeper’s larvae (Bostrichthys sinensis, Lacepede, 1881)

2019 ◽  
Vol 41 (2) ◽  
Author(s):  
Pham Thanh Cong ◽  
Hoang Thi Lan Anh ◽  
Dang Diem Hong
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Polyunsaturated Fatty Acid ◽  
Total Fatty Acid ◽  
Seed Quality ◽  
Brachionus Plicatilis ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Significant Difference

 Schizochytrium mangrovei PQ6 contains many important polyunsaturated fatty acids, such as docosahexaenoic acid (DHA, C22: 6w-3), eicosahexaenoic acid (EPA, C20: 5w-3) and docosapentaenoic acid (DPA, C22: 5w-6). These fatty acids are essential for survival and growth of many marine fish larvae. In this paper, fresh biomass of S. mangrovei PQ6 was used for culturing rotifer Brachionus plicatilis. In the first formula (L1), rotifers were fed on baker’s yeast; in second formula (L2), rotifers were fed on mixed two microalgae, Nannochloropsis oculata and Chaetoceros gracilis; in the third formula (L3), rotifers were fed on S. mangrovei PQ6 biomass. The results indicated that S. mangrovei PQ6 biomass can replace baker’s yeast and autotrophic microalgae in feeding rotifers. Total lipid, total fatty acid and polyunsaturated fatty acid contents of rotifers fed on formula 3 (S. mangrovei PQ6) were higher than those within others. High polyunsaturated fatty acid (C ≥ 20) in omega-3 and 6 groups; DHA and DPA contents of rotifers fed on S. mangrovei PQ6 were the highest, accounting for 39.81% of total fatty acid; 41.95% and 8.24% polyunsaturated fatty acids, corresponding to 20.52; 12.15 and 2.4 mg/g of dried weight, respectively.The survival rate of black sleeper’s larvae was highest when they were fed on rotifers grown on L3 formula (51.20 ± 0.89%), followed by L2 formula (48.70 ± 2.67%) and the lowest with L1 formula (43.44 ± 1.54%) (P < 0.05). However, among three formulas, no significant difference was found in the growth of black sleeper’s larvae (P > 0.05). These results suggest that biomass of S. mangrovei PQ6 can replace traditional feed like autotrophic microalgae or baker’s yeast for biomass culture of the Brachionus plicatilis rotifer for rearing black sleeper’s larvae to enhance seed quality to meet demands of market. 

scholarly journals Fatty Acid Compositions of Mixed Microalgae from Tilapia Fish Ponds

2019 ◽  
pp. 1-7
Author(s):  
Norhafizah Osman ◽  
Hishamuddin Omar
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Acid Methyl Ester ◽  
Monounsaturated Fatty Acids ◽  
Major Constituent ◽  
Fish Ponds ◽  
Fatty Acid Profiles ◽  
Fatty Acid Compositions

Microalgae has been getting broad attention of researchers and investors lately, especially when discussing on healthy food and energy sources for the future. In this study, twelve samples of mixed microalgae from outdoor ponds were analyzed for their fatty acid compositions. The potential of microalgae to solve variety of world’s problems was not realized because of bottleneck in microalgal supplies at reasonable cost. Therefore, the objective of this study is to determine fatty acid profiles of mixed microalgae from tilapia fish ponds. The study was conducted in Tapak Ternakan Ikan, Taman Pertanian Universiti and Department of Biology, Faculty of Science, Universiti Putra Malaysia. Mixed microalgae were extracted for their lipids with methanol: chloroform mixture and after transesterification, the fatty acid methyl ester were analyzed using gas chromatography equipped with flame ionization detector. Results showed that saturated was the major constituent fatty acids. The average percentages of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids obtained were 45.62 ± 1.37%, 20.05 ± 1.14%, and 34.33 ± 3.17% respectively. The most dominant fatty acid profiles were C18:3n3 (α-linolenic acid) and C16:0 (palmitic acid), with the overall percentages of 19.97% and 19.40% respectively. The fatty acid profiles of mixed microalgae was good with a decent balance of saturated, monounsaturated and polyunsaturated fatty acids.

scholarly journals Discarded fish on the Spanish Mediterranean coast: influence of season on fatty acids profiles

2021 ◽  
Vol 22 (2) ◽  
pp. 232
Author(s):  
MARÍA DOLORES SUÁREZ ◽  
MARÍA ISABEL SÁEZ ◽  
MIGUEL ÁNGEL RINCÓN-CERVERA ◽  
LUIS HIDALGO ◽  
JOSE LUIS GUIL-GUERRERO
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Mediterranean Coast ◽  
Total N ◽  
Fa Composition ◽  
Potential Use ◽  
Long Chain

This work focused on determining the fatty acid (FA) composition of commonly discarded fish on the Spanish Mediterranean coast in winter and summer to assess their potential use as raw sources of very long-chain polyunsaturated fatty acids (VLCPUFAs). Total n-3 VLCPUFA content significantly varies depending on capture season, and values were higher in winter for Pomadasys incisus (1.36 g/100 g), Chelidonichthys lucerna (1.67 g/100 g) and Trachinus draco (2.04 g/100 g), while Pagellus acarne had larger n-3 PUFA amounts in summer (3.89 g/100 g). Generally for most species, monounsaturated FA, eicosapentaenoic acid (20:5n-3) and total FA had higher values in summer, while both the n-3 VLCPUFA fraction and DHA content were higher in winter. Knowledge of these changes allows the processes for their proper use as valuable PUFA sources to be adjusted. The discarded fish herein studied could be generally considered to be raw n-3 VLCPUFAs sources.

Pengasingan Strain Baru Kulat dari Tanah Di Malaysia untuk Penghasilan Asid γ –Linolenik (GLA)

2012 ◽  
Author(s):  
A. A. Hamid ◽  
W. M. W. Yusof ◽  
R. M. Illias ◽  
K. Nadarajah
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Profiles ◽  
Forest Reserves ◽  
Evening Primrose ◽  
Oleaginous Fungi ◽  
Lipid Contents ◽  
Total Fatty Acids ◽  
The Difference

Kajian telah dijalankan untuk mendapatkan strain baru kulat yang menghasilkan GLA hasil penyaringan 21 pencilan yang diperoleh daripada tanah hutan simpan di sekitar Selangor dan Negeri Sembilan. Sebanyak 5 pencilan kulat daripada 21 pencilan yang diperoleh telah dikenalpasti menghasilkan asid γ–linolenik (GLA) (18:3, n-6) iaitu SK8, SK22, 2A1, DR dan K3, yang menghasilkan 6%, 7.6%, 6.9% 5.4% dan 13.5% GLA daripada asid lemak total masing-masing. Kelima-lima pencilan tersebut juga didapati menghasilkan peratusan lipid yang berbeza, iaitu 30%, 20%, 32%, 30% dan 8% lipid total (w/w, biojisim), masing-masing. Pencilan K3 mempunyai kandungan GLA yang setara dengan kandungan GLA dalam minyak ‘Evening Primrose’ yang dihasilkan secara komersial pada masa kini, iaitu 8-12% GLA dari asid lemak total. Pencilan 2A1 menunjukkan penghasilan GLA tertinggi (0.0096g GLA/g glukosa) berbanding SKA, SK22, DR dan K3, yang menghasilkan 0.0032. 0.0039, 0.007 dan 0.0025g, GLA/g glukosa, masing-masing. Perbezaan profil asid lemak bagi keseluruhan 21 pencilan juga dibincangkan. Kata kunci: Oleaginous; kulat; γ-linolenic; asid lemak poli-tidak-tepu. In our studies, 21 filamentous fungi were isolated from soil obtained from various sites of forest reserves in Selangor and Negeri Sembilan. Five isolates (SK8, SK22, 2A1, DR and K3) were found to produced γ–linolenic acid (GLA) (18:3, n–6) at 6%, 7.6%, 6.9%, 5.4% and 13.5% of their respective total fatty acid with lipid contents of 30%, 20%, 32%, 30% and 8% (w/w, biomass), respectively. The GLA content of K3 is comparable to that of the commercially available ‘Evening Primrose’ with GLA comprising of 8–12% of its total fatty acids. The difference in the fatty acid profiles from all 21 isolates is discussed here. Key words: Oleaginous; fungi γ-linolenic; polyunsaturated fatty acids.

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