Identification and Characterization of an IgG Binding Protein in the Secretion of the Rat Coagulating Gland

2002 ◽  
Vol 383 (12) ◽  
pp. 1959-1965 ◽  
Author(s):  
B. Wilhelm ◽  
C. Keppler ◽  
A. Henkeler ◽  
M. Schilli-Westermann ◽  
D. Linder ◽  
...  

AbstractA merocrine released protein (named 115k protein) was highly enriched from the secretion of the rat coagulating gland. The protein has a molecular mass of 115 kDa as calculated by SDSPAGE under reducing conditions. Furthermore, the 115 kDa protein is glycosylated, and carries Man, GlcNAc, Gal, Fuc and sialic acid residues. For identification, Nterminal amino acid and nucleotide sequence analyses were performed. The sequences obtained showed 86 to 100% identity with human and mouse IgGFc binding proteins. The functional capacity of IgG binding of the 115 kDa protein was shown by overlay experiments, indicating its membership in the IgG binding protein family.

2002 ◽  
Vol 301 (2) ◽  
pp. 705-713 ◽  
Author(s):  
Fang L. Zhang ◽  
Lin Luo ◽  
Eric Gustafson ◽  
Kyle Palmer ◽  
Xudong Qiao ◽  
...  

1998 ◽  
Vol 273 (17) ◽  
pp. 10325-10330 ◽  
Author(s):  
Kelley A. Ruud ◽  
Christopher Kuhlow ◽  
Dixie J. Goss ◽  
Karen S. Browning

2010 ◽  
Vol 25 (2) ◽  
pp. 150-156 ◽  
Author(s):  
J. Vorrasi ◽  
B. Chaudhuri ◽  
E.M. Haase ◽  
F.A. Scannapieco

2006 ◽  
Vol 96 (5) ◽  
pp. 542-548 ◽  
Author(s):  
Marcel Maymon ◽  
Aida Zveibil ◽  
Shimon Pivonia ◽  
Dror Minz ◽  
Stanley Freeman

Sixty-four isolates of Colletotrichum gloeosporioides were isolated from infected Limonium spp. cultivated in 12 different locations in Israel. All isolates were identified as belonging to the C. gloeosporioides complex by species-specific primers. Of these isolates, 46 were resistant to benomyl at 10 μg/ml and 18 were sensitive to this concentration of fungicide. Based on arbitrarily primed polymerase chain reaction of all isolates and internal transcribed spacer-1 sequence analyses of 12 selected isolates, the benomyl-resistant and -sensitive populations belong to two distinct genotypes. Sequence analyses of the β-tubulin genes, TUB1 and TUB2, of five sensitive and five resistant representative isolates of C. gloeosporioides from Limonium spp. revealed that the benomyl-resistant isolates had an alanine substitute instead of a glutamic acid at position 198 in TUB2. All data suggest that the resistant and sensitive genotypes are two independent and separate populations. Because all Limonium plant propagation material is imported from various geographic regions worldwide, and benomyl is not applied to this crop or for the control of Colletotrichum spp. in Israel, it is presumed that plants are bearing quiescent infections from the points of origin prior to arrival.


Acta Tropica ◽  
2018 ◽  
Vol 182 ◽  
pp. 69-79 ◽  
Author(s):  
Dolores A. Ayón-Núñez ◽  
Gladis Fragoso ◽  
Clara Espitia ◽  
Martín García-Varela ◽  
Xavier Soberón ◽  
...  

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