Characterization of RNase P Holoenzymes from Methanococcus jannaschii and Methanothermobacter thermoautotrophicus

2001 ◽  
Vol 382 (8) ◽  
Author(s):  
Andrew J. Andrews ◽  
Thomas A. Hall ◽  
James W. Brown
Keyword(s):  
Rnase P ◽  
Methanococcus Jannaschii ◽  
Methanothermobacter Thermoautotrophicus

Partial characterization of an RNA component that copurifies with Saccharomyces cerevisiae RNase P

1989 ◽  
Vol 9 (6) ◽  
pp. 2536-2543
Author(s):  
J Y Lee ◽  
D R Engelke
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Secondary Structure ◽  
Schizosaccharomyces Pombe ◽  
Rnase P ◽  
Partial Characterization ◽  
Rna Sequences ◽  
Extensive Sequence ◽  
Sequence Similarities

Saccharomyces cerevisiae cellular RNase P is composed of both protein and RNA components that are essential for activity. The isolated holoenzyme contains a highly structured RNA of 369 nucleotides that has extensive sequence similarities to the 286-nucleotide RNA associated with Schizosaccharomyces pombe RNase P but bears little resemblance to the analogous RNA sequences in procaryotes or S. cerevisiae mitochondria. Even so, the predicted secondary structure of S. cerevisiae RNA is strikingly similar to the bacterial phylogenetic consensus rather than to previously predicted structures of other eucaryotic RNase P RNAs.

scholarly journals Functional reconstitution and characterization of Pyrococcus furiosus RNase P

2006 ◽  
Vol 103 (44) ◽  
pp. 16147-16152 ◽  
Author(s):  
H.-Y. Tsai ◽  
D. K. Pulukkunat ◽  
W. K. Woznick ◽  
V. Gopalan
Keyword(s):  
Pyrococcus Furiosus ◽  
Rnase P

scholarly journals Characterization of RPR1, an essential gene encoding the RNA component of Saccharomyces cerevisiae nuclear RNase P.

1991 ◽  
Vol 11 (2) ◽  
pp. 721-730 ◽  
Author(s):  
J Y Lee ◽  
C E Rohlman ◽  
L A Molony ◽  
D R Engelke
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Essential Gene ◽  
Rnase P ◽  
Single Copy ◽  
Temperature Sensitive ◽  
Coding Region ◽  
Gene Encoding ◽  
Processing Steps ◽  
Potential Precursor

RNA components have been identified in preparations of RNase P from a number of eucaryotic sources, but final proof that these RNAs are true RNase P subunits has been elusive because the eucaryotic RNAs, unlike the procaryotic RNase P ribozymes, have not been shown to have catalytic activity in the absence of protein. We previously identified such an RNA component in Saccharomyces cerevisiae nuclear RNase P preparations and have now characterized the corresponding, chromosomal gene, called RPR1 (RNase P ribonucleoprotein 1). Gene disruption experiments showed RPR1 to be single copy and essential. Characterization of the gene region located RPR1 600 bp downstream of the URA3 coding region on chromosome V. We have sequenced 400 bp upstream and 550 bp downstream of the region encoding the major 369-nucleotide RPR1 RNA. The presence of less abundant, potential precursor RNAs with an extra 84 nucleotides of 5' leader and up to 30 nucleotides of 3' trailing sequences suggests that the primary RPR1 transcript is subjected to multiple processing steps to obtain the 369-nucleotide form. Complementation of RPR1-disrupted haploids with one variant of RPR1 gave a slow-growth and temperature-sensitive phenotype. This strain accumulates tRNA precursors that lack the 5' end maturation performed by RNase P, providing direct evidence that RPR1 RNA is an essential component of this enzyme.

scholarly journals Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP

1998 ◽  
Vol 12 (11) ◽  
pp. 1678-1690 ◽  
Author(s):  
J. R. Chamberlain ◽  
Y. Lee ◽  
W. S. Lane ◽  
D. R. Engelke
Keyword(s):  
Rnase P ◽  
Purification And Characterization ◽  
Rnase Mrp

scholarly journals Characterization of yeast mitochondrial RNase P: an intact RNA subunit is not essential for activityin vitro

1989 ◽  
Vol 17 (17) ◽  
pp. 6865-6881 ◽  
Author(s):  
Michael J. Morales ◽  
Carol A. Wise ◽  
Margaret J. Hollingsworth ◽  
Nancy C. Martin
Keyword(s):  
Rnase P

Partial purification and characterization of RNase P from human peripheral lymphocytes

2009 ◽  
Vol 18 (2) ◽  
pp. 130-133 ◽  
Author(s):  
Anastassios Vourekas ◽  
Eleftheria Vryzaki ◽  
Chrisavgi Toumpeki ◽  
Vassiliki Stamatopoulou ◽  
Alexandra Monastirli ◽  
...  
Keyword(s):  
Rnase P ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Peripheral Lymphocytes ◽  
Human Peripheral Lymphocytes
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