scholarly journals Minor changes largely restore catalytic activity of archaeal RNase P RNA from Methanothermobacter thermoautotrophicus

2008 ◽  
Vol 37 (1) ◽  
pp. 231-242 ◽  
Author(s):  
Dan Li ◽  
Dagmar K. Willkomm ◽  
Roland K. Hartmann
Keyword(s):  
Catalytic Activity ◽  
Rnase P ◽  
Methanothermobacter Thermoautotrophicus

scholarly journals The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA fromPyrococcus horikoshiiOT3

2011 ◽  
Vol 75 (4) ◽  
pp. 816-819 ◽  
Author(s):  
Tadashi HARA ◽  
Atsushi TERADA ◽  
Hiroki YAMAGUCHI ◽  
Takashi NAKASHIMA ◽  
Yoshimitsu KAKUTA ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Rnase P

Extensive deproteinization of Dictyostelium discoideum RNase P reveals a new catalytic activity

2001 ◽  
Vol 268 (7) ◽  
pp. 2134-2140 ◽  
Author(s):  
Constantinos Stathopoulos ◽  
Apostolos Tekos ◽  
Ioannis K. Zarkadis ◽  
Denis Drainas
Keyword(s):  
Catalytic Activity ◽  
Dictyostelium Discoideum ◽  
Rnase P

scholarly journals Interactions between RNase P protein subunits in Archaea

Archaea ◽  
2004 ◽  
Vol 1 (4) ◽  
pp. 247-254 ◽  
Author(s):  
Thomas A. Hall ◽  
James W. Brown
Keyword(s):  
Protein Interactions ◽  
Rnase P ◽  
Ribonuclease P ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Protein Subunits ◽  
P Protein ◽  
Yeast Two Hybrid System ◽  
Methanothermobacter Thermoautotrophicus ◽  
Two Hybrid

A yeast two-hybrid system was used to identify protein–protein interactions between the ribonuclease P (RNase P) protein subunits Mth11p, Mth687p, Mth688p and Mth1618p from the archaeonMethanothermobacter thermoautotrophicus. Clear interactions between Mth688p and Mth687p, and between Mth1618p and Mth11p, were confirmed byHIS3andLacZreporter expression. Weaker interactions of Mth687p and Mth688p with Mth11p, and Mth11p with itself, are also suggested. These interactions resemble, and confirm, those previously seen among the homologs of these proteins in the more complex yeast RNase P holoenzyme.

RNase P RNA-mediated catalysis

2002 ◽  
Vol 30 (6) ◽  
pp. 1153-1158 ◽  
Author(s):  
L. A. Kirsebom
Keyword(s):  
Catalytic Activity ◽  
Metal Ions ◽  
Specific Interaction ◽  
Rnase P ◽  
Substrate Complex ◽  
Mediated Catalysis

The endoribonuclease RNase P is involved in the processing of tRNA precursors to generate mature 5′ termini. The catalytic activity of RNase P is associated with an RNA, RNase P RNA. A specific interaction between the 3′ end of the substrate and RNase P RNA, to form an RNase P RNA-substrate complex, is referred to as the ‘73–294-interaction’. This interaction has an important role for efficient and correct cleavage to occur. Here our understanding of the contribution of the 73–294-interaction and metal ions, with respect to efficient and correct cleavage in RNase P RNA-mediated catalysis, will be discussed.

High resolution electron microscopy of lanthanum phosphate crystals

1978 ◽  
Vol 36 (1) ◽  
pp. 274-275
Author(s):  
J. C. Wheatley ◽  
J. M. Cowley
Keyword(s):  
Electron Microscopy ◽  
Catalytic Activity ◽  
High Resolution ◽  
Petroleum Industry ◽  
High Resolution Electron Microscopy ◽  
Lanthanum Phosphate ◽  
Good Catalyst ◽  
Resolution Electron ◽  
Good Catalytic Activity ◽  
Physical And Chemical

Rare-earth phosphates are of particular interest because of their catalytic properties associated with the hydrolysis of many aromatic chlorides in the petroleum industry. Lanthanum phosphates (LaPO4) which have been doped with small amounts of copper have shown increased catalytic activity (1). However the physical and chemical characteristics of the samples leading to good catalytic activity are not known.Many catalysts are amorphous and thus do not easily lend themselves to methods of investigation which would include electron microscopy. However, the LaPO4, crystals are quite suitable samples for high resolution techniques.The samples used were obtained from William L. Kehl of Gulf Research and Development Company. The electron microscopy was carried out on a JEOL JEM-100B which had been modified for high resolution microscopy (2). Standard high resolution techniques were employed. Three different sample types were observed: 669A-1-5-7 (poor catalyst), H-L-2 (good catalyst) and 27-011 (good catalyst).

The design and catalytic performance of molybdenum active sites on an MCM-41 framework for the aerobic oxidation of 5-hydroxymethylfurfural to 2,5-diformylfuran

2019 ◽  
Vol 9 (3) ◽  
pp. 811-821 ◽  
Author(s):  
Zhao-Meng Wang ◽  
Li-Juan Liu ◽  
Bo Xiang ◽  
Yue Wang ◽  
Ya-Jing Lyu ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Active Sites ◽  
Aerobic Oxidation ◽  
Catalytic Performance ◽  
Mcm 41

The catalytic activity decreases as –(SiO)3Mo(OH)(O) > –(SiO)2Mo(O)2 > –(O)4–MoO.

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