The PCR and nested PCR detection of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Dermacentor reticulatus F. collected in a new location in Poland (Trzciel, Western Poland)

2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Patrycja Opalińska ◽  
Anna Wierzbicka ◽  
Marek Asman
Keyword(s):  
Tree Species ◽  
Nested Pcr ◽  
Anaplasma Phagocytophilum ◽  
Dermacentor Reticulatus ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Forest Landscape ◽  
The West ◽  
West Part ◽  
New Location

AbstractThe study was performed in the Trzciel Forest Districts which is located in the west part of Poland. The Scots pine is the main tree species creating forest landscape there.

scholarly journals Novel Protozoans in Austria Revealed through the Use of Dogs as Sentinels for Ticks and Tick-Borne Pathogens

2021 ◽  
Vol 9 (7) ◽  
pp. 1392
Author(s):  
Michiel Wijnveld ◽  
Anna-Margarita Schötta ◽  
Theresa Stelzer ◽  
Georg Duscher ◽  
Michael Leschnik ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Blot Hybridization ◽  
Dermacentor Reticulatus ◽  
Sensu Stricto ◽  
Babesia Microti ◽  
Rickettsia Helvetica ◽  
Haemaphysalis Concinna ◽  
Borrelia Lusitaniae ◽  
Rickettsia Monacensis ◽  
Borrelia Burgdorferi Sensu Stricto

We previously isolated and cultivated the novel Rickettsia raoultii strain Jongejan. This prompted us to ask whether this strain is unique or more widely present in Austria. To assess this issue, we retrospectively screened ticks collected from dogs in 2008. Of these collected ticks, we randomly selected 75 (47 females and 28 males) Dermacentor reticulatus, 44 (21 females, 7 males, and 16 nymphs) Haemaphysalis concinna, and 55 (52 females and 3 males) ticks of the Ixodes ricinus complex. Subsequently, these ticks were individually screened for the presence of tick-borne pathogens using the reverse line blot hybridization assay. In our current study, we detected DNA from the following microbes in D. reticulatus: Anaplasma phagocytophilum, Borrelia lusitaniae, Borrelia spielmanii, Borrelia valaisiana, and R. raoultii, all of which were R. raoultii strain Jongejan. In H. concinna, we found DNA of a Babesia sp., Rickettsia helvetica, and an organism closely related to Theileria capreoli. Lastly, I. ricinus was positive for Anaplasma phagocytophilum, Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii/Borrelia bavariensis, B. lusitaniae, B. spielmanii, B. valaisiana, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, Rickettsia monacensis, and Theileria (Babesia) microti DNA. The detection of DNA of the Babesia sp. and an organism closely related to Theileria capreoli, both found in H. concinna ticks, is novel for Austria.

scholarly journals Detection of Borrelia burgdorferi s.l., Anaplasma phagocytophilum and Babesia spp. in Dermacentor reticulatus ticks found within the city of Białystok, Poland—first data

2021 ◽  
Vol 85 (1) ◽  
pp. 63-73
Author(s):  
Anna Grochowska ◽  
Justyna Dunaj ◽  
Sławomir Pancewicz ◽  
Piotr Czupryna ◽  
Piotr Majewski ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Animal Health ◽  
Dermacentor Reticulatus ◽  
Babesia Microti ◽  
Rural Landscapes ◽  
Babesia Vogeli ◽  
Borrelia Burgdorferi S.L ◽  
Babesia Venatorum ◽  
The City

AbstractPathogens carried by ticks pose a threat to both human and animal health across the world. Typically associated with rural landscapes, ticks appear to adapt well to life in urban recreational areas. Although Dermacentor reticulatus is commonly found across Europe, data on the prevalence of pathogens in this tick species, in an urban environment, are very limited. PCR was used to examine 368 D. reticulatus individuals collected in the Zwierzyniecki Forest Nature Reserve in Białystok, Poland. In total, 10.3% of ticks were infected, with Babesia spp. (9.2%), Anaplasma phagocytophilum (0.8%) and Borrelia burgdorferi sensu lato (0.3%). Rickettsia spp., Bartonella spp., and Coxiella burnetii were not detected. Sequence analysis for Babesia-positive samples identified 79.4% of them as Babesia canis, 8.8% as Babesia microti, 5.9% as Babesia spp., 2.9% as Babesia venatorum, and 2.9% as Babesia vogeli. Results obtained in this study indicate that D. reticulatus ticks found within the urban premises of the study area are infected with at least three pathogens and therefore are an important factor in public health risk for tick-borne diseases.

scholarly journals Detection of Anaplasma phagocytophilum, Babesia odocoilei, Babesia sp., Borrelia burgdorferi Sensu Lato, and Hepatozoon canis in Ixodes scapularis Ticks Collected in Eastern Canada

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1265
Author(s):  
John D. Scott ◽  
Risa R. Pesapane
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Ixodes Scapularis ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Hepatozoon Canis ◽  
Eastern Canada ◽  
Zoonotic Pathogens ◽  
First Time ◽  
Established Population

Tick-borne pathogens cause infectious diseases that inflict much societal and financial hardship worldwide. Blacklegged ticks, Ixodes scapularis, are primary vectors of several epizootic and zoonotic pathogens. The aim sets forth the pathogens and their prevalence. In Ontario and Quebec, 113 I. scapularis ticks were collected from songbirds, mammals, including humans, and by flagging. PCR and DNA sequencing detected five different microorganisms: Anaplasma phagocytophilum, 1 (0.9%); Babesia odocoilei, 17 (15.3%); Babesia microti-like sp., 1 (0.9%); Borrelia burgdorferi sensu lato (Bbsl), 29 (26.1%); and Hepatozoon canis, 1 (0.9%). Five coinfections of Bbsl and Babesia odocoilei occurred. Notably, H. canis was documented for the first time in Canada and, at the same time, demonstrates the first transstadial passage of H. canis in I. scapularis. Transstadial passage of Bbsl and B. odocoilei was also witnessed. A novel undescribed piroplasm (Babesia microti-like) was detected. An established population of I. scapularis ticks was detected at Ste-Anne-de-Bellevue, Quebec. Because songbirds widely disperse I. scapularis larvae and nymphs, exposure in an endemic area is not required to contract tick-borne zoonoses. Based on the diversity of zoonotic pathogens in I. scapularis ticks, clinicians need to be aware that people who are bitten by I. scapularis ticks may require select antimicrobial regimens.

scholarly journals Molecular Detection of Borrelia burgdorferi Sensu Lato and Anaplasma phagocytophilum in Ticks Collected from Dogs in Urban Areas of North-Eastern Poland

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 455
Author(s):  
Mirosław M. Michalski ◽  
Katarzyna Kubiak ◽  
Magdalena Szczotko ◽  
Marta Chajęcka ◽  
Małgorzata Dmitryjuk
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Urban Areas ◽  
Molecular Detection ◽  
Dermacentor Reticulatus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
16S Rna ◽  
North Eastern ◽  
The City ◽  
Rna Genes

From 2016 to 2018, ticks were collected from 272 dogs admitted to veterinary clinics in the city of Olsztyn (north-eastern Poland). Among 522 collected ticks, 423 were identified as Ixodes ricinus (413 females and 10 males) and 99 as Dermacentor reticulatus (62 females and 37 males). Non-engorged (86 individuals) and engorged (436 individuals) ticks were screened for the presence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum DNA. Borrelia and A. phagocytophilum species detection was determined based on the sequence of the fla B and 16S RNA genes, respectively. DNA of B. burgdorferi s.l. was identified in 31.6% (165/522, 95% CI: 27.6–35.8%) of ticks (I. ricinus 151/423, 35.7%, 95% CI: 31.1–40.4%; D. reticulates 14/99, 14.1%, 95% CI: 7.9–22.6%). A. phagocytophilum was identified in 0.96% (5/522, 95% CI: 0.3–2.2%) of specimens. All positive samples were engorged I. ricinus females (5/402, 1.2%, 95% CI: 0.4–2.9%). In 85.4% (141/165, 95% CI: 79.1–90.4%) of Borrelia infected ticks, the DNA of one genospecies was revealed. The DNA of at least two different genospecies was detected in 14.5% of specimens (24/165, 95% CI: 9.5–20.8). The coexistence of B. burgdorferii s.l. and A. phagocytophilum was not detected.

scholarly journals Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows of Lubelskie Voivodship (eastern Poland)

2014 ◽  
Vol 58 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Beata Dzięgiel ◽  
Tomasz Kubrak ◽  
Łukasz Adaszek ◽  
Piotr Dębiak ◽  
Dagmara Wyłupek ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Dermacentor Reticulatus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Eastern Region ◽  
Babesia Canis ◽  
Area At Risk ◽  
Adult Females

Abstract The aim of the study was to assess the distribution of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Babesia canis in adult females and males of Ixodes ricinus and Dermacentor reticulatus ticks, inhabiting meadows near large forest complexes throughout the Lubelskie Voivodship (eastern region of Poland). Ticks were collected using the flagging method. Among 720 ticks collected, 506 were identified as D. reticulatus, and 214 as I. ricinus. DNA of B. canis and B. burgdorferi s.l. was detected in 21.3% and 0.6% of D. reticulatus ticks, respectively. In I. ricinus ticks, DNA specific to B. burgdorferi s.l. and A. phagocytophilum was detected in 5.6% and 10.3%, respectively. Co-infections of B. burgdorferi s.l. and A. phagocytophilum were found in two I. ricinus ticks. These results indicate that the Lublin region is an area at risk of tick-borne diseases of humans and animals, which must be considered in clinical practice.

scholarly journals The occurrence of three tick-borne pathogens in Ixodes ricinus ticks collected from the area of the Kraków-Częstochowa Upland (Southern Poland)

Acarologia ◽  
2018 ◽  
Vol 58 (4) ◽  
pp. 969-975
Author(s):  
Marek Asman ◽  
Krzysztof Solarz ◽  
Ewa Szilman ◽  
Piotr Szilman ◽  
Bartosz Sikora ◽  
...  
Keyword(s):  
Ixodes Ricinus ◽  
Potential Risk ◽  
Anaplasma Phagocytophilum ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Infectious Agents ◽  
Ixodes Ricinus Ticks ◽  
Risk Of Exposure ◽  
The Common ◽  
The City

The common tick Ixodes ricinus is the main vector of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Europe. The aim of this study was to estimate the potential risk of exposure to common tick-borne diseases in the recreational areas of the Kraków-Częstochowa Upland, Poland with particular emphasis on the city of Częstochowa. The DNA from 459 I. ricinus ticks was used to detect B. burgdorferi s. l., A. phagocytophilum and B. microti by PCR and nested PCR methods. Generally, infectious agents were found in 26.3% of all the examined ticks: B. microti was found in 23.3%, and A. phagocytophilum in 2.4% of the ticks. The protozoan was found mainly in females and nymphs, while A. phagocytophilum was detected mainly in adults. The co-existence of B. microti and A. phagocytophilum was found in 2.1% of the examined ticks. The presence of B. burgdorferi s. l. was not revealed in the examined material. The conducted studies demonstrate the high potential risk of exposure of tourists and pilgrims to B. microti, and a low risk of exposure to A. phagocytophilum and B. burgdorferi s.l. in the examined areas of the Kraków-Częstochowa Upland. Furthermore, the possible co-existence of A. phagocytophilum and B. microti in 3 individual ticks means that infection by multiple infectious agents is possible. The obtained results highlight the need to conduct further research on tick-borne pathogens in this region of Poland.

Application of GIS, Hydraulic and Hydrologic Modelling to Investigate the Changes of Flood Inundation Characteristics due to the Construction of Dams for different scenarios

2018 ◽  
Vol 1 (2) ◽  
pp. 61-77
Author(s):  
Hossameldin M. Elhanafy
Keyword(s):  
Fluid Mechanics ◽  
Flood Control ◽  
Reduction Rate ◽  
Hydraulic Modeling ◽  
Direct Result ◽  
Flood Inundation ◽  
High Ability ◽  
The West ◽  
Hydrologic Modelling ◽  
New Location

The novelty of the research project reported in this paper is the coupling of hydrological and hydraulic modeling which are based on the first principal of fluid mechanics for the simulation of flash floods at Wadi Elarish watershed to optimize the a new location of another dam rather than Elrawfa dam which already exist. Results show that, the optimum scenario is obtained by the construction of the west dam. As a direct result of this dam, the downstream inundated area can be reduced up to 15.7 % as function of reservoir available storage behind the dam. Furthermore, calculations showed that the reduction rate of inundated area for 50-year floods is largely more than 100-year floods, implies the high ability of west dam on flood control especially for floods with shorter return period.

Excavations in Midea 2007

2009 ◽  
Vol 2 ◽  
pp. 7-30 ◽  
Author(s):  
Katie Demakopoulou ◽  
Nicoletta Divari-Valakou ◽  
Monica Nilsson ◽  
Ann-Louise Schallin
Keyword(s):  
Bronze Age ◽  
Early Phase ◽  
The West ◽  
Large Section ◽  
Ample Evidence ◽  
West Part ◽  
Large Opening

Excavations in Midea continued in 2007 as a Greek-Swedish programme under the direction of Dr Katie Demakopoulou in collaboration with Dr Ann-Louise Schallin. In the West Gate area excavation continued in the west part of the building complex that abuts the fortification wall. Room XIV was excavated with abundant remains of LH IIIB2 pottery. A sealstone with a unique, possibly ritual, scene was also found. On the lower west terrace of the acropolis excavation continued in Trench C, where a large section of the fortification wall was uncovered. Room I was excavated here, adjacent to the inner face of the fortification wall. Finds in this room date to the early phase of LH IIIC, under which there was ample evidence of the LH IIIB2 destruction, including human skeletons. Under this debris, a large opening leading to a gallery or syrinx through the thickness of the fortification wall was found. Excavation was resumed also in the East Gate area, where a new wall was revealed in the baulk between Trench 3 and Room 9. The wall is perpendicular to the citadel wall and borders Trench 3. Excavation was also resumed in Trenches 9 and 14. The latest Mycenaean material in this area dates to LH IIIB2, but there is evidence of post-Bronze Age activity, which is demonstrated mainly by pottery finds.

Peregrinação Acadêmica: A mulher Romeira do Bom Jesus da Lapa

2020 ◽  
Author(s):  
Sandra Célia Coelho Gomes da Silva
Keyword(s):  
Gender Identity ◽  
Social Reproduction ◽  
Participant Observation ◽  
Female Gender ◽  
Age Group ◽  
Structured Interviews ◽  
The West ◽  
Patriarchal Family ◽  
The Family ◽  
West Part

This work is the result of the doctoral thesis entitled Pilgrimage of Bom Jesus da Lapa: Social Reproduction of the Family and Female Gender Identity, specifically the second chapter that talks about women in the Pilgrimage of Bom Jesus da Lapa, emphasizing gender relations, analyzing the location of the pilgrimage as a social reproduction of the patriarchal family and female gender identity. The research scenario is the Bom Jesus da Lapa Pilgrimage, which has been held for 329 years, in that city, located in the West part of Bahia. The research participants are pilgrim women who are in the age group between 50 and 70 years old and have participated, for more than five consecutive years in the Bom Jesus da Lapa Pilgrimage, belonging to five Brazilian states (Bahia, Minas Gerais, São Paulo, Espírito Santo and Goiás) that register a higher frequency of attendance at this religious event. We used bibliographic, qualitative, field and documentary research and data collection as our methodology; we applied participant observation and semi-structured interviews as a technique. We concluded that the Bom Jesus da Lapa Pilgrimage is a location for family social reproduction and the female gender identity, observing a contrast in the resignification of the role and in the profile of the pilgrim women from Bom Jesus da Lapa, alternating between permanence and the transformation of gender identity coming from patriarchy.

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