scholarly journals Molecular Detection of Borrelia burgdorferi Sensu Lato and Anaplasma phagocytophilum in Ticks Collected from Dogs in Urban Areas of North-Eastern Poland

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 455
Author(s):  
Mirosław M. Michalski ◽  
Katarzyna Kubiak ◽  
Magdalena Szczotko ◽  
Marta Chajęcka ◽  
Małgorzata Dmitryjuk
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Urban Areas ◽  
Molecular Detection ◽  
Dermacentor Reticulatus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
16S Rna ◽  
North Eastern ◽  
The City ◽  
Rna Genes

From 2016 to 2018, ticks were collected from 272 dogs admitted to veterinary clinics in the city of Olsztyn (north-eastern Poland). Among 522 collected ticks, 423 were identified as Ixodes ricinus (413 females and 10 males) and 99 as Dermacentor reticulatus (62 females and 37 males). Non-engorged (86 individuals) and engorged (436 individuals) ticks were screened for the presence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum DNA. Borrelia and A. phagocytophilum species detection was determined based on the sequence of the fla B and 16S RNA genes, respectively. DNA of B. burgdorferi s.l. was identified in 31.6% (165/522, 95% CI: 27.6–35.8%) of ticks (I. ricinus 151/423, 35.7%, 95% CI: 31.1–40.4%; D. reticulates 14/99, 14.1%, 95% CI: 7.9–22.6%). A. phagocytophilum was identified in 0.96% (5/522, 95% CI: 0.3–2.2%) of specimens. All positive samples were engorged I. ricinus females (5/402, 1.2%, 95% CI: 0.4–2.9%). In 85.4% (141/165, 95% CI: 79.1–90.4%) of Borrelia infected ticks, the DNA of one genospecies was revealed. The DNA of at least two different genospecies was detected in 14.5% of specimens (24/165, 95% CI: 9.5–20.8). The coexistence of B. burgdorferii s.l. and A. phagocytophilum was not detected.

scholarly journals Detection of Borrelia burgdorferi s.l., Anaplasma phagocytophilum and Babesia spp. in Dermacentor reticulatus ticks found within the city of Białystok, Poland—first data

2021 ◽  
Vol 85 (1) ◽  
pp. 63-73
Author(s):  
Anna Grochowska ◽  
Justyna Dunaj ◽  
Sławomir Pancewicz ◽  
Piotr Czupryna ◽  
Piotr Majewski ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Animal Health ◽  
Dermacentor Reticulatus ◽  
Babesia Microti ◽  
Rural Landscapes ◽  
Babesia Vogeli ◽  
Borrelia Burgdorferi S.L ◽  
Babesia Venatorum ◽  
The City

AbstractPathogens carried by ticks pose a threat to both human and animal health across the world. Typically associated with rural landscapes, ticks appear to adapt well to life in urban recreational areas. Although Dermacentor reticulatus is commonly found across Europe, data on the prevalence of pathogens in this tick species, in an urban environment, are very limited. PCR was used to examine 368 D. reticulatus individuals collected in the Zwierzyniecki Forest Nature Reserve in Białystok, Poland. In total, 10.3% of ticks were infected, with Babesia spp. (9.2%), Anaplasma phagocytophilum (0.8%) and Borrelia burgdorferi sensu lato (0.3%). Rickettsia spp., Bartonella spp., and Coxiella burnetii were not detected. Sequence analysis for Babesia-positive samples identified 79.4% of them as Babesia canis, 8.8% as Babesia microti, 5.9% as Babesia spp., 2.9% as Babesia venatorum, and 2.9% as Babesia vogeli. Results obtained in this study indicate that D. reticulatus ticks found within the urban premises of the study area are infected with at least three pathogens and therefore are an important factor in public health risk for tick-borne diseases.

scholarly journals Molecular detection of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato genospecies in red foxes (Vulpes vulpes) from Romania

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Mirabela Oana Dumitrache ◽  
Ioana Adriana Matei ◽  
Angela Monica Ionică ◽  
Zsuzsa Kalmár ◽  
Gianluca D’Amico ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Vulpes Vulpes ◽  
Molecular Detection ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Red Foxes

scholarly journals Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows of Lubelskie Voivodship (eastern Poland)

2014 ◽  
Vol 58 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Beata Dzięgiel ◽  
Tomasz Kubrak ◽  
Łukasz Adaszek ◽  
Piotr Dębiak ◽  
Dagmara Wyłupek ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Dermacentor Reticulatus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Eastern Region ◽  
Babesia Canis ◽  
Area At Risk ◽  
Adult Females

Abstract The aim of the study was to assess the distribution of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Babesia canis in adult females and males of Ixodes ricinus and Dermacentor reticulatus ticks, inhabiting meadows near large forest complexes throughout the Lubelskie Voivodship (eastern region of Poland). Ticks were collected using the flagging method. Among 720 ticks collected, 506 were identified as D. reticulatus, and 214 as I. ricinus. DNA of B. canis and B. burgdorferi s.l. was detected in 21.3% and 0.6% of D. reticulatus ticks, respectively. In I. ricinus ticks, DNA specific to B. burgdorferi s.l. and A. phagocytophilum was detected in 5.6% and 10.3%, respectively. Co-infections of B. burgdorferi s.l. and A. phagocytophilum were found in two I. ricinus ticks. These results indicate that the Lublin region is an area at risk of tick-borne diseases of humans and animals, which must be considered in clinical practice.

scholarly journals Serological Evidence of Natural Exposure to Tick-Borne Pathogens in Horses, Romania

2021 ◽  
Vol 9 (2) ◽  
pp. 373
Author(s):  
Andreea Monica Bogdan ◽  
Mariana Ionita ◽  
Ioan Liviu Mitrea
Keyword(s):  
Confidence Interval ◽  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Serological Evidence ◽  
North Central ◽  
Natural Exposure

The purpose of this study was to investigate the seroprevalence of selected tick-borne-pathogens (TBPs) among Romanian horses. For this, a total of 223 animals originating from north, central, and southeast Romania, including horses from stud farms (n = 118) and working horses (n = 105), were tested using a commercial rapid ELISA-based test. Overall, 10.3% (95% confidence interval (CI): 6.7–15.1%) of the tested horses were seropositive for antibodies (Ab) against Anaplasma phagocytophilum. Additionally, 18.8% (95% CI: 13.9–24.6%) and 0.5% (95% CI: 0.01–2.5%) of horses were seropositive for Ab against Borrelia burgdorferi sensu lato and Ehrlichia spp., respectively. Among the tested horses, 3.1% were seroreactive to two or three pathogens. These findings show the natural exposure of Romanian horses to zoonotic tick-borne pathogens and emphasize the need for further studies to better understand the epidemiology of equine tick-borne diseases in Romania.

scholarly journals Tick-borne pathogens in Ixodidae ticks collected from privately-owned dogs in Italy: a country-wide molecular survey

2020 ◽  
Author(s):  
Stefania Zanet ◽  
Elena Battisti ◽  
Paola Pepe ◽  
Lavinia Ciuca ◽  
Liliana Colombo ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Areas ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Specific Pcr ◽  
The Family ◽  
Borrelia Burgdorferi S.L ◽  
Privately Owned ◽  
Prevalent Species ◽  
Minimum Infection Rate ◽  
Theileria Spp

Abstract Background Ticks and tick-borne diseases are increasingly recognized as a cause of disease in dogs worldwide. The epidemiology of ticks and tick-transmitted protozoa and bacteria has changed due to the spread of ticks to urban and peri-urban areas and the movement of infected animals, posing new risks for animals and humans. This countrywide study reports information on distribution and prevalence of pathogens in ticks collected from privately-owned dogs in Italy. We analyzed 2681 Ixodidae ticks, collected from 1454 pet dogs from Italy. Specific PCR protocols were used to detect i) Piroplasms of the genera Babesia and Theileria , ii) Gram-negative cocci of the family Anaplasmataceae and iii) Borrelia burgdorferi sensu lato. Sequencing of positive amplicons allowed for species identification. Results Babesia / Theileria spp. DNA was detected in 435 homogeneous tick-pools (Minimum Infection Rate (MIR) = 27.6%; 95% confidence interval (CI) = 25.4-29.8%) with higher prevalence in Ixodes ricinus and Rhipicephalus sanguneus group. The zoonotic B. venatorum was the most prevalent species (MIR = 7.5%; 95% CI = 6.3-9.0%). Anaplasma and Ehrlichia species were detected in 165 tick-pools (MIR = 10.5%; 95% CI = 9.3-11.8%) and specifically, A. phagocytophilum was identified with MIR = 5.1% (95% CI = 4.1-6.3%). Borrelia burgdorferi s.l. and B. afzelii were detected with MIR = 0.4% (95% CI = 0.2-0.8%) and MIR = 0.3% (95% CI 0.1-0.7%) respectively. Conclusions Zoonotic pathogens B. venatorum and A. phagocytophilum were the most frequently detected in ticks collected from privately-owned dogs which might be used as markers of pathogens presence and distribution.

scholarly journals Tick species and tick-borne infections identified in population from a rural area of Spain

2005 ◽  
Vol 133 (5) ◽  
pp. 943-949 ◽  
Author(s):  
FRANCISCO JESÚS MERINO ◽  
TERESA NEBREDA ◽  
JOSE LUIS SERRANO ◽  
PEDRO FERNÁNDEZ-SOTO ◽  
ANTONIO ENCINAS ◽  
...  
Keyword(s):  
Health Care ◽  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Tick Species ◽  
Medical Advice ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Dermacentor Marginatus ◽  
Rickettsia Slovaca ◽  
Rickettsia Massiliae ◽  
Rhipicephalus Bursa

To determine the tick species that bite humans in the province of Soria (Spain) and ascertain the tick-borne pathogens that threaten people's health in that province, 185 tick specimens were collected from 179 patients who sought medical advice at health-care centres. The ticks were identified, and their DNA examined by PCR for pathogens. Most ticks were collected in autumn and spring (59 and 57 respectively). Nine species of ticks were identified, the most frequent being Dermacentor marginatus (55·7%), Ixodes ricinus (12·4%) and Rhipicephalus bursa (11·9%). Ninety-seven females, 66 males, 21 nymphs and one larva were identified. Twenty-six ticks carried DNA from Rickettsia spp. (11 Rickettsia slovaca, 6 Rickettsia spp. RpA4/DnS14, 1 Rickettsia massiliae/Bar29, and 8 unidentified); two ticks carried DNA from Borrelia burgdorferi sensu lato and seven ticks harboured DNA from Anaplasma phagocytophilum.

scholarly journals Questing Ixodes ricinus ticks (Acari, Ixodidae) as a vector of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in an urban area of north-eastern Poland

2019 ◽  
Vol 78 (1) ◽  
pp. 113-126 ◽  
Author(s):  
Katarzyna Kubiak ◽  
Janina Dziekońska-Rynko ◽  
Hanna Szymańska ◽  
Dariusz Kubiak ◽  
Małgorzata Dmitryjuk ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Area ◽  
Ixodes Ricinus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Borrelia Miyamotoi ◽  
North Eastern ◽  
Ixodes Ricinus Ticks

Seasonal variations in detecting Borrelia burgdorferi sensu lato in rodents from north eastern Austria

2006 ◽  
Vol 118 (23-24) ◽  
pp. 754-758 ◽  
Author(s):  
Gelas Khanakah ◽  
Elena Kocianová ◽  
Vanda Vyrosteková ◽  
Jozef Řeháček ◽  
Michael Kundi ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Seasonal Variations ◽  
Borrelia Burgdorferi Sensu Lato ◽  
North Eastern ◽  
Eastern Austria
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