scholarly journals Determination of adulterants in adulterant-fruit spirit blends using excitation-emission matrix fluorescence spectroscopy

2015 ◽  
Vol 8 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Michaela Jakubíková ◽  
Jana Sádecká ◽  
Pavel Májek
Keyword(s):  
Wavelength Range ◽  
Fluorescence Spectra ◽  
Reliable Method ◽  
Excitation Wavelength ◽  
Mean Square ◽  
Excitation Emission Matrix ◽  
Ethanol Level ◽  
Parallel Factor ◽  
Eem Fluorescence

Abstract This study introduces a reliable method to detect adulteration of spirit drinks. Excitation-emission matrix (EEM) fluorescence in combination with parallel factor analysis (PARAFAC) and partial least squares (PLS) regression was used to determine the content of water and ethanol in adulterated fruit spirit samples. EEM fluorescence spectra recorded in the emission wavelength range of 315–450 nm and in the excitation wavelength range of 240–305 nm were used for PARAFAC. The model created using PARAFAC-PLS was able to predict the water and ethanol level in adulterated apple spirit with the root mean square error of prediction (RMSEP) values of 1.9 % and 1.8 %, respectively. Regarding adulterated plum spirit, the RMSEP values of 0.7 % and 3.5 % were obtained for water and ethanol, respectively. The aim of this work was to determine whether EEM-PARAFAC can be used to distinguish between plum and apple spirit. Better results were obtained for apple spirit and the method is useful also for water-apple spirit blends.

Rapid determination of sulfamethoxazole and trimethoprim illegally added into health products using excitation-emission matrix fluorescence coupled with second-order calibration method

2021 ◽  
Author(s):  
Lu-Zhu Chen ◽  
Hai-Long Wu ◽  
WanJun Long ◽  
Tong Wang ◽  
Gao-Yan Tong ◽  
...  
Keyword(s):  
Analytical Method ◽  
Rapid Determination ◽  
Calibration Method ◽  
Trilinear Decomposition ◽  
Excitation Emission Matrix ◽  
Health Products ◽  
Alternating Trilinear Decomposition ◽  
Second Order Calibration ◽  
Eem Fluorescence

In this work, a simple and fast analytical method based on self-weighted alternating trilinear decomposition (SWATLD) algorithm coupled with excitation-emission matrix (EEM) fluorescence was developed for the simultaneous determination of...

Determination of Gentamicin Residual in Duck Meat Using Fluorescence Analysis Method

2014 ◽  
Vol 1033-1034 ◽  
pp. 638-642
Author(s):  
Xiao Wang ◽  
Jiang Xu ◽  
Mu Hua Liu ◽  
Jin Hui Zhao ◽  
Qian Hong
Keyword(s):  
Correlation Coefficient ◽  
Fluorescence Spectra ◽  
Limit Of Detection ◽  
Fluorescence Analysis ◽  
Excitation Wavelength ◽  
Analysis Method ◽  
Standard Samples ◽  
Meat Extract ◽  
Coefficient Of Regression

Gentamicin is a kind of aminoglycoside antibiotics and widely used in the prevention and treatment of the duck diseases. A prediction model was established for the rapid detection of Gentamicin residue in duck meat using fluorescence analysis method according to the strong fluorescent characteristic of the generated derivative for Gentamicin and o-phthaldialdehyde (OPA) in the presence of emulsifier OP-10 and mercaptoethanol.The fluorescence spectra of the duck meat containing Gentamicin were analyzed, the optimum excitation wavelength of the material was 340 nm and the optimum emission wavelength was 442 nm. Fluorescence intensity and the concentration of the standard samples presented the good linear relationship, the linear correlation coefficient was 0.9963 and the limit of detection was 0.47 μg/mL in the dynamic rang of 0.5 ~6.5μg/mL. The correlation coefficient of regression equation was 0.9968 for the samples of duck meat extract. The experimental results showed that the fluorescence analysis method had a good performance and accuracy in detecting the Gentamicin residue in duck meat.

3DEEM spectroscopy analysis to assess the EPS composition in different carriers in HMBR systems

2016 ◽  
Vol 74 (11) ◽  
pp. 2708-2716 ◽  
Author(s):  
Meixiang Sun ◽  
Man Wu ◽  
Wen Liu ◽  
Huiying Liu ◽  
Yezhong Zhang ◽  
...  
Keyword(s):  
Humic Acid ◽  
Fulvic Acid ◽  
Membrane Fouling ◽  
Fluorescence Spectra ◽  
Membrane Bioreactor ◽  
Extracellular Polymeric Substances ◽  
Three Dimensional ◽  
Spectroscopy Analysis ◽  
Excitation Emission Matrix ◽  
Parallel Factor

A hybrid membrane bioreactor (HMBR) with biological band carriers (Reactor A) and an HMBR with suspended honeycomb carriers (Reactor B) were conducted in parallel to investigate the effects of different carriers on extracellular polymeric substances (EPS). Composition and concentration of EPS were examined by three-dimensional excitation-emission matrix (3DEEM) fluorescence spectra and parallel factor analysis (PARAFAC). 3DEEM spectra demonstrated that the main organic substances of the EPS in two reactors were protein-like, humic acid-like and fulvic acid-like substances. The fluorescence intensity (FI) indicated that the protein-like composition was dominant in EPS, and its intensity in reactor B was stronger than that in A (392.94 > 250.25). Results of the FI identified from the 3DEEM by PARAFAC showed that the EPS in two reactors included two humic acid-like compositions C1 (230, 320/406 nm), C2 (250, 360/440 nm) and one protein-like C4 (230, 280/340 nm), while C3 was fulvic acid-like (220/429 nm) and protein-like (230/357 nm) in reactor A and B, respectively. The proportion and FI of protein-like substances in reactor B were higher than that in A. Consequently, it was concluded that reactor A could control the membrane fouling effectively, compared with reactor B.

Brown carbon aerosols in the Indo-Gangetic Plain outflow: insights from excitation emission matrix (EEM) fluorescence spectroscopy

2021 ◽  
Author(s):  
Supriya Dey ◽  
Arya Mukherjee ◽  
Anuraag J Polana ◽  
Archita Rana ◽  
Jingying Mao ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Gangetic Plain ◽  
Brown Carbon ◽  
Excitation Emission Matrix ◽  
Indian Context ◽  
Parallel Factor ◽  
Carbon Aerosols ◽  
Indo Gangetic Plain ◽  
Eem Fluorescence

We report the first characterization of aerosol brown carbon (BrC) composition in the Indian context using excitation emission matrix (EEM) fluorescence spectroscopy coupled with parallel factor (PARAFAC) analysis. We find...

scholarly journals HPLC separation and determination of dicoumarol and other simple coumarins in sweet clover

2018 ◽  
Vol 17 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Katarína Hroboňová ◽  
Jana Sádecká ◽  
Jozef Čižmárik
Keyword(s):  
Fluorescence Spectra ◽  
Correlation Coefficients ◽  
Gradient Elution ◽  
Excitation Wavelength ◽  
Sweet Clover ◽  
System Suitability ◽  
Synchronous Fluorescence Spectra ◽  
Peak Areas ◽  
Microbial Action

AbstractDicoumarol is a mycotoxin, that acts as a blood anticoagulant, is formed during the microbial action of molds and fungi in spoiled hay or silage containing high-coumarin plant. A HPLC-DAD method for determination of coumarins, including dicoumarol, coumarin, and 4-hydroxycoumarin was developed. Methanol and acetic acid were used as mobile phase with gradient elution. The simultaneous separation was performed using C18 type of stationary phase. The recoveries were 88.6 – 92.6 %, 91.8 – 95.0 %, and 89.7 – 94.1 % (evaluated for three concentration levels) for dicoumarol, coumarin, and 4-hydroxycoumarin respectively. The parameters of system suitability (repeatability of retention times and peak areas) were determined for evaluation of the method. The method showed a good linearity in the concentration range 0.7 – 100 μg.mL−1for dicoumarol, 0.05 – 100 μg.mL−1for coumarin and 4-hydroxycoumarin with correlation coefficients higher than 0.9885. Extracts of sweet clover herb, hay, and spoiled hay were subjected to HPLC-DAD analysis. The most abundant compound in sweet clover herb and hay extracts was coumarin. In spoiled sweet clover hay extract the 4-hydroxycoumarin was detected in addition. The formation of 4-hydroxycoumarin was also observed in the synchronous fluorescence spectra recorded at the wavelength difference of 90 nm (difference between emission and excitation wavelength).

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