3DEEM spectroscopy analysis to assess the EPS composition in different carriers in HMBR systems

2016 ◽  
Vol 74 (11) ◽  
pp. 2708-2716 ◽  
Author(s):  
Meixiang Sun ◽  
Man Wu ◽  
Wen Liu ◽  
Huiying Liu ◽  
Yezhong Zhang ◽  
...  
Keyword(s):  
Humic Acid ◽  
Fulvic Acid ◽  
Membrane Fouling ◽  
Fluorescence Spectra ◽  
Membrane Bioreactor ◽  
Extracellular Polymeric Substances ◽  
Three Dimensional ◽  
Spectroscopy Analysis ◽  
Excitation Emission Matrix ◽  
Parallel Factor

A hybrid membrane bioreactor (HMBR) with biological band carriers (Reactor A) and an HMBR with suspended honeycomb carriers (Reactor B) were conducted in parallel to investigate the effects of different carriers on extracellular polymeric substances (EPS). Composition and concentration of EPS were examined by three-dimensional excitation-emission matrix (3DEEM) fluorescence spectra and parallel factor analysis (PARAFAC). 3DEEM spectra demonstrated that the main organic substances of the EPS in two reactors were protein-like, humic acid-like and fulvic acid-like substances. The fluorescence intensity (FI) indicated that the protein-like composition was dominant in EPS, and its intensity in reactor B was stronger than that in A (392.94 > 250.25). Results of the FI identified from the 3DEEM by PARAFAC showed that the EPS in two reactors included two humic acid-like compositions C1 (230, 320/406 nm), C2 (250, 360/440 nm) and one protein-like C4 (230, 280/340 nm), while C3 was fulvic acid-like (220/429 nm) and protein-like (230/357 nm) in reactor A and B, respectively. The proportion and FI of protein-like substances in reactor B were higher than that in A. Consequently, it was concluded that reactor A could control the membrane fouling effectively, compared with reactor B.

Optimization for extracellular polymeric substances extraction of microbial aggregates

2015 ◽  
Vol 71 (7) ◽  
pp. 1106-1112 ◽  
Author(s):  
Liang Zhu ◽  
Haitian Yu ◽  
Yimei Liu ◽  
Hanying Qi ◽  
Xiangyang Xu
Keyword(s):  
Humic Acid ◽  
Extracellular Polymeric Substances ◽  
Three Dimensional ◽  
Interaction Mechanism ◽  
Extraction Methods ◽  
Aerobic Granules ◽  
Excitation Emission Matrix ◽  
Cation Exchange Resins ◽  
Heat Method ◽  
Exchange Resins

The extracellular polymeric substances (EPS) are important macromolecular components in microbial aggregates. The three EPS extraction methods – ultrasound + cation exchange resins (CER) + sulfide, ultrasound + formamide + NaOH, and ultrasound + heat – were investigated in the study, and the component differences of extracted EPS from the loose flocs and dense aerobic granules were compared using chemical analysis and three-dimensional excitation-emission matrix (3D-EEM). Results showed that the contents of EPS were extracted effectively by ultrasound + formamide + NaOH and ultrasound + heat methods, and the ultrasound + CER + sulfide method did not extract the polysaccharides (PS) or protein (PN) contents from the sludge samples. The 3D-EEM analysis indicated that the nature of peak B/D, peak C/E/F, and peak A/G were attributed to PN-like, humic acid-like and fulvic acid-like fluorophores. All fluorophores can be detected from the EPS extracted through the ultrasound + heat method. Hopefully this will provide more information about the EPS interaction mechanism of microbial aggregates.

Enhanced Performance of Ultrafiltration Membrane with Powdered Zeolite Addition for Secondary Effluent Treatment

2013 ◽  
Vol 838-841 ◽  
pp. 2712-2716
Author(s):  
Yong Tu ◽  
Yong Gang Bai ◽  
Yong Chen ◽  
Wei Jing Liu ◽  
Jun Xu ◽  
...  
Keyword(s):  
Membrane Fouling ◽  
Fluorescence Spectra ◽  
Municipal Wastewater ◽  
Three Dimensional ◽  
Treatment Plant ◽  
Ultrafiltration Membrane ◽  
Effluent Treatment ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
Sem Images

The research on ultrafiltration membrane assisted by powdered zeolite for the treatment of secondary effluent from a municipal wastewater treatment plant was studied. The results show that membrane fouling rate is reduced by pre-coating the ultrafiltration membrane with powdered zeolite, and the treatment performance of secondary effluent is enhanced. UV-vis, three-dimensional excitation emission matrix (3D-EEM) fluorescence spectra and scanning electron microscopy (SEM) images for ultrafiltration were also discussed.

Investigation of long-term operation and biomass activity in a membrane bioreactor system

2011 ◽  
Vol 63 (9) ◽  
pp. 1906-1912 ◽  
Author(s):  
Simos Malamis ◽  
Andreas Andreadakis ◽  
Daniel Mamais ◽  
Constantinos Noutsopoulos
Keyword(s):  
Membrane Fouling ◽  
Membrane Bioreactor ◽  
Extracellular Polymeric Substances ◽  
Operating Conditions ◽  
Term Operation ◽  
Solids Retention ◽  
Biomass Activity ◽  
Long Term Performance ◽  
Term Performance

The aim of this work was to evaluate the long-term performance of a Membrane Bioreactor (MBR) that operated continuously for 2.5 years and to assess membrane fouling and biomass activity under various operating conditions. Furthermore, a method for the characterisation of influent wastewater was developed based on its separation into various fractions. The MBR system operated at the solids retention times (SRT) of 10, 15, 20 and 33 days. The increase of SRT resulted in a decrease of the fouling rate associated with the reduction of extracellular polymeric substances. Moreover, the SRT increase resulted in a significant reduction of the Oxygen Uptake Rate (OUR) due to the lower availability of substrate and in a notable decrease of the maximum OUR since high SRT allowed the development of slower growing microorganisms. Biomass consisted of small flocs due to extensive deflocculation caused by intense aeration. Finally, the method developed for wastewater characterisation is straightforward and less time consuming than the usual method that is employed.

scholarly journals Biomass Characteristics and Their Effect on Membrane Bioreactor Fouling

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2867 ◽  
Author(s):  
Petros K. Gkotsis ◽  
Anastasios I. Zouboulis
Keyword(s):  
Membrane Fouling ◽  
Membrane Bioreactor ◽  
Suspended Solids ◽  
Extracellular Polymeric Substances ◽  
Membrane Surface ◽  
Membrane Resistance ◽  
Soluble Microbial Products ◽  
Gelling Properties ◽  
Microbial Products ◽  
Soluble Part

Biomass characteristics are regarded as particularly influential for fouling in Membrane Bio-Reactors (MBRs). They primarily include the Mixed Liquor Suspended Solids (MLSS), the colloids and the Extracellular Polymeric Substances (EPS). Among them, the soluble part of EPS, which is also known as Soluble Microbial Products (SMP), is the most significant foulant, i.e., it is principally responsible for membrane fouling and affects all fundamental fouling indices, such as the Trans-Membrane Pressure (TMP) and the membrane resistance and permeability. Recent research in the field of MBRs, tends to consider the carbohydrate fraction of SMP (SMPc) the most important characteristic for fouling, mainly due to the hydrophilic and gelling properties, which are exhibited by polysaccharides and allow them to be easily attached on the membrane surface. Other wastewater and biomass characteristics, which affect indirectly membrane fouling, include temperature, viscosity, dissolved oxygen (DO), foaming, hydrophobicity and surface charge. The main methods employed for the characterization and assessment of biomass quality, in terms of filterability and fouling potential, can be divided into direct (such as FDT, SFI, TTF100, MFI, DFCM) or indirect (such as CST, TOC, PSA, RH) methods, and they are shortly presented in this review.

Performance of membrane bioreactor combined with pre-coagulation/sedimentation

2004 ◽  
Vol 4 (1) ◽  
pp. 143-149 ◽  
Author(s):  
T. Itonaga ◽  
Y. Watanabe
Keyword(s):  
Membrane Fouling ◽  
Membrane Bioreactor ◽  
Suspended Solids ◽  
Extracellular Polymeric Substances ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Municipal Wastewater Treatment ◽  
Term Operation ◽  
Made In

This paper deals with the performance of a hybrid membrane bioreactor (MBR) combined with pre-coagulation/sedimentation. Primary clarifier effluent in a municipal wastewater treatment plant was fed into the hybrid MBR to investigate its performance during long-term operation. Pre-coagulation/sedimentation process efficiently removed the suspended solids including organic matter and phosphorus. Comparison of the hybrid MBR and conventional MBR was made in terms of the permeate quality and membrane fouling. As the organic loading to the MBR was significantly reduced by the pre-coagulation/sedimentation, production and accumulation of extracellular polymeric substances (EPS) may be limited. Therefore, the mixed liquor viscosity in the hybrid MBR was much lower than that in the conventional MBR. These effect caused by pre-coagulation/sedimentation brought a remarkable improvement in both permeate quality and membrane permeability.

scholarly journals Swine manure treatment by anaerobic membrane bioreactor with carbon, nitrogen and phosphorus recovery

2017 ◽  
Vol 76 (8) ◽  
pp. 1939-1949 ◽  
Author(s):  
Fan Bu ◽  
Shiyun Du ◽  
Li Xie ◽  
Rong Cao ◽  
Qi Zhou
Keyword(s):  
Relaxation Rate ◽  
Membrane Fouling ◽  
Membrane Bioreactor ◽  
Extracellular Polymeric Substances ◽  
Swine Manure ◽  
Phosphorus Recovery ◽  
Methane Yield ◽  
Continuous Stirred Tank Reactor ◽  
Chemical Cleaning ◽  
Anaerobic Membrane Bioreactor

Swine manure wastewater was treated in an anaerobic membrane bioreactor (AnMBR) that combined a continuous stirred tank reactor (CSTR) and a hollow-fiber ultrafiltration membrane, and the feasibility of ammonia and phosphorus recovery in the permeate was investigated. The AnMBR system was operated steadily with a high mixed liquor suspended solids (MLSS) concentration of 32.32 ± 6.24 g/L for 120 days, achieving an average methane yield of 280 mL/gVSadded and total chemical oxygen demand removal efficiency of 96%. The methane yield of the AnMBR is 83% higher than that of the single CSTR. The membrane fouling mechanism was examined, and MLSS and the polysaccharide contents of the extracellular polymeric substances were found to be the direct causes of membrane fouling. The effects of the permeation/relaxation rate and physical, chemical cleaning on membrane fouling were assessed for membrane fouling control, and results showed that a decrease in the permeation/relaxation rate together with chemical cleaning effectively reduced membrane fouling. In addition, a crystallization process was used for ammonia and phosphorus recovery from the permeate, and pH 9 was the optimal condition for struvite formation. The study has an instructive significance to the industrial applications of AnMBRs in treating high strength wastewater with nutrient recovery.

Effect of Extracellular Polymeric Substances on Operation of Membrane Bioreactor

2012 ◽  
Vol 549 ◽  
pp. 491-495
Author(s):  
Wan You Zhang ◽  
Xin Yan Wang ◽  
Li Juan Xi
Keyword(s):  
Membrane Fouling ◽  
Membrane Bioreactor ◽  
Extracellular Polymeric Substances ◽  
Membrane Resistance ◽  
Volume Index ◽  
Direct Influence ◽  
Sludge Volume Index ◽  
Simple Method ◽  
Modified Fouling Index ◽  
The Relationship

In order to study the relationship between extracellular polymeric substances (EPS) and membrane fouling, the effect of extracellular polymeric substances (EPS) on the operation of membrane bioreactor (MBR) was investigated in this paper. The operation of membrane was analyzed by evaluating sludge volume index (SVI), modified fouling index (MFI), and membrane resistance (Rt), respectively. The results showed that SVI, MFI, and Rt increased with the accumulation of EPS, and membrane fouling aggravated with the increase of EPS, this illustrated that the content of EPS had a direct influence on SVI, MFI, Rt and membrane fouling. The consequences could offer a simple method to monitor the concentration of EPS by analyzing SVI, MFI, or Rt.

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