scholarly journals Ellagic acid promotes browning of white adipose tissues in high-fat diet-induced obesity in rats through suppressing white adipocyte maintaining genes

2019 ◽  
Vol 66 (10) ◽  
pp. 923-936 ◽  
Author(s):  
Lifeng Wang ◽  
Yuanyuan Wei ◽  
Chao Ning ◽  
Minfang Zhang ◽  
Ping Fan ◽  
...  
Keyword(s):  
Ellagic Acid ◽  
High Fat Diet ◽  
High Fat ◽  
Adipose Tissues ◽  
White Adipocyte ◽  
Diet Induced Obesity

scholarly journals High-Fat Diet Alters the Expression of Reference Genes in Male Mice

2020 ◽  
Vol 7 ◽  
Author(s):  
Xiuqin Fan ◽  
Hongyang Yao ◽  
Xuanyi Liu ◽  
Qiaoyu Shi ◽  
Liang Lv ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Reference Genes ◽  
Molecular Mechanisms ◽  
Obese Mouse ◽  
Mrna Levels ◽  
High Fat ◽  
Adipose Tissues ◽  
Obesity Epidemic ◽  
Diet Induced Obesity

Quantitative PCR (qPCR), the most accurate and sensitive technique for quantifying mRNA expression, and choice of appropriate reference genes for internal error controlling in qPCR are essential to understanding the molecular mechanisms that drive the obesity epidemic and its comorbidities. In this study, using the high-fat diet (HFD)-induced obese mouse model, we assessed the expression of 10 commonly used reference genes to validate gene-expression stability in adipose tissue, liver, and muscle across different time points (4, 8, 12, and 16 weeks after HFD feeding) during the process of obesity. The data were analyzed by the GeNorm, NormFinder, BestKeeper, and Delta-Ct method, and the results showed that the most stable reference genes were different for a specific organ or tissue in a specific time point; however, PPIA, RPLP0, and YWHAZ were the top three most stable reference genes in qPCR experiments on adipose, hepatic tissues, and muscles of mice in diet-induced obesity. In addition, the mostly used genes ACTB and GAPDH were more unstable in the fat and liver, the ACTB mRNA levels were increased in four adipose tissues, and the GAPDH mRNA levels were decreased in four adipose tissues and liver after HFD feeding. These results suggest that PPIA, RPLP0, or YWHAZ may be more appropriate to be used as reference gene than ACTB and GAPDH in the adipose tissue and liver of mice during the process of high-fat diet-induced obesity.

scholarly journals Effects of Curcumin in a Mouse Model of Very High Fat Diet-Induced Obesity

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1368
Author(s):  
Iurii Koboziev ◽  
Shane Scoggin ◽  
Xiaoxia Gong ◽  
Parvin Mirzaei ◽  
Masoud Zabet-Moghaddam ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Protective Effects ◽  
Metabolic Dysfunction ◽  
High Fat ◽  
Dietary Interventions ◽  
Experimental Conditions ◽  
White Adipocyte ◽  
Diet Induced Obesity ◽  
Invaluable Tool ◽  
Very High

Worldwide rates of Western-diet-induced obesity epidemics are growing dramatically. Being linked with numerous comorbidities and complications, including cardiovascular disease, type 2 diabetes, cancer, chronic inflammation, and osteoarthritis (OA), obesity represents one of the most threatening challenges for modern healthcare. Mouse models are an invaluable tool for investigating the effects of diets and their bioactive components against high fat diet (HFD)-induced obesity and its comorbidities. During recent years, very high fat diets (VHFDs), providing 58–60% kcal fat, have become a popular alternative to more traditional HFDs, providing 40–45% total kcal fat, due to the faster induction of obesity and stronger metabolic responses. This project aims to investigate if the 60% fat VHFD is suitable to evaluate the protective effects of curcumin in diet-induced obesity and osteoarthritis. B6 male mice, prone to diet-induced metabolic dysfunction, were supplemented with VHFD without or with curcumin for 13 weeks. Under these experimental conditions, feeding mice a VHFD for 13 weeks did not result in expected robust manifestations of the targeted pathophysiologic conditions. Supplementing the diet with curcumin, in turn, protected the animals against obesity without significant changes in white adipocyte size, glucose clearance, and knee cartilage integrity. Additional research is needed to optimize diet composition, curcumin dosage, and duration of dietary interventions to establish the VHFD-induced obesity for evaluating the effects of curcumin on metabolic dysfunctions related to obesity and osteoarthritis.

scholarly journals Cajanolactone A, a Stilbenoid From Cajanus canjan (L.) Millsp, Prevents High-Fat Diet-Induced Obesity via Suppressing Energy Intake

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhuohui Luo ◽  
Jiawen Huang ◽  
Zhiping Li ◽  
Zhiwen Liu ◽  
Linchun Fu ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Energy Intake ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Drug Candidate ◽  
High Fat ◽  
Adipose Tissues ◽  
Fat Accumulation ◽  
Diet Induced Obesity ◽  
Brown Adipose

Cajanolactone A (CLA) is a stilbenoid isolated from Cajanus canjan (L.) Millsp with the potential to prevent postmenopausal obesity. In this study, the effect of CLA on high-fat diet (HFD)-induced obesity in female C57BL/6 mice was investigated. It was found that, treatment with CLA reduced the energy intake and effectively protected the mice from HFD-induced body weight gain, fat accumulation within the adipose tissues and liver, and impairment in energy metabolism. Further investigation revealed that CLA significantly down-regulated the expression of ORX, ORXR2, pMCH, and Gal in the hypothalamus and antagonized HFD-induced changes in the expression of UCP1, Pgc-1α, Tfam, and Mfn1 in the inguinal white adipose tissue (iWAT); Caveolin-1, MT and UCP3 in the perigonadal white adipose tissue (pWAT); and Pdhb, IRS2, Mttp, Hadhb, and Cpt1b in the liver. CLA also protected the pWAT and liver from HFD-induced mitochondrial damage. However, neither HFD nor CLA showed an effect on the mass of brown adipose tissue (BAT) or the expression of UCP1 in the BAT. In summary, our findings suggest that CLA is a potential drug candidate for preventing diet-induced obesity, at least in females. CLA works most likely by suppressing the hypothalamic expression of orexigenic genes, which leads to reduced energy intake, and subsequently, reduced fat accumulation, thereby protecting the adipose tissues and the liver from lipid-induced mitochondrial dysfunction.

scholarly journals Deletion of PPAR  in adipose tissues of mice protects against high fat diet-induced obesity and insulin resistance

2005 ◽  
Vol 102 (17) ◽  
pp. 6207-6212 ◽  
Author(s):  
J. R. Jones ◽  
C. Barrick ◽  
K.-A. Kim ◽  
J. Lindner ◽  
B. Blondeau ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
High Fat Diet ◽  
High Fat ◽  
Adipose Tissues ◽  
Diet Induced Obesity

Glucagon under high fat diet induced obesity

2007 ◽  
Vol 115 (S 1) ◽  
Author(s):  
LC Bollheimer ◽  
H Wobser ◽  
CE Wrede ◽  
A Schäffler ◽  
J Schölmerich ◽  
...  
Keyword(s):  
High Fat Diet ◽  
High Fat ◽  
Diet Induced Obesity
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