Overexpression of bone morphogenetic protein-3b (BMP-3b) in adipose tissues protects against high-fat diet-induced obesity

2017 ◽  
Vol 41 (4) ◽  
pp. 483-488 ◽  
Author(s):  
J Hino ◽  
M Nakatani ◽  
Y Arai ◽  
K Tsuchida ◽  
M Shirai ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
High Fat Diet ◽  
High Fat ◽  
Adipose Tissues ◽  
Diet Induced Obesity ◽  
Morphogenetic Protein

scholarly journals High-Fat Diet Alters the Expression of Reference Genes in Male Mice

2020 ◽  
Vol 7 ◽  
Author(s):  
Xiuqin Fan ◽  
Hongyang Yao ◽  
Xuanyi Liu ◽  
Qiaoyu Shi ◽  
Liang Lv ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Reference Genes ◽  
Molecular Mechanisms ◽  
Obese Mouse ◽  
Mrna Levels ◽  
High Fat ◽  
Adipose Tissues ◽  
Obesity Epidemic ◽  
Diet Induced Obesity

Quantitative PCR (qPCR), the most accurate and sensitive technique for quantifying mRNA expression, and choice of appropriate reference genes for internal error controlling in qPCR are essential to understanding the molecular mechanisms that drive the obesity epidemic and its comorbidities. In this study, using the high-fat diet (HFD)-induced obese mouse model, we assessed the expression of 10 commonly used reference genes to validate gene-expression stability in adipose tissue, liver, and muscle across different time points (4, 8, 12, and 16 weeks after HFD feeding) during the process of obesity. The data were analyzed by the GeNorm, NormFinder, BestKeeper, and Delta-Ct method, and the results showed that the most stable reference genes were different for a specific organ or tissue in a specific time point; however, PPIA, RPLP0, and YWHAZ were the top three most stable reference genes in qPCR experiments on adipose, hepatic tissues, and muscles of mice in diet-induced obesity. In addition, the mostly used genes ACTB and GAPDH were more unstable in the fat and liver, the ACTB mRNA levels were increased in four adipose tissues, and the GAPDH mRNA levels were decreased in four adipose tissues and liver after HFD feeding. These results suggest that PPIA, RPLP0, or YWHAZ may be more appropriate to be used as reference gene than ACTB and GAPDH in the adipose tissue and liver of mice during the process of high-fat diet-induced obesity.

scholarly journals Ellagic acid promotes browning of white adipose tissues in high-fat diet-induced obesity in rats through suppressing white adipocyte maintaining genes

2019 ◽  
Vol 66 (10) ◽  
pp. 923-936 ◽  
Author(s):  
Lifeng Wang ◽  
Yuanyuan Wei ◽  
Chao Ning ◽  
Minfang Zhang ◽  
Ping Fan ◽  
...  
Keyword(s):  
Ellagic Acid ◽  
High Fat Diet ◽  
High Fat ◽  
Adipose Tissues ◽  
White Adipocyte ◽  
Diet Induced Obesity

scholarly journals Cajanolactone A, a Stilbenoid From Cajanus canjan (L.) Millsp, Prevents High-Fat Diet-Induced Obesity via Suppressing Energy Intake

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhuohui Luo ◽  
Jiawen Huang ◽  
Zhiping Li ◽  
Zhiwen Liu ◽  
Linchun Fu ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Energy Intake ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Drug Candidate ◽  
High Fat ◽  
Adipose Tissues ◽  
Fat Accumulation ◽  
Diet Induced Obesity ◽  
Brown Adipose

Cajanolactone A (CLA) is a stilbenoid isolated from Cajanus canjan (L.) Millsp with the potential to prevent postmenopausal obesity. In this study, the effect of CLA on high-fat diet (HFD)-induced obesity in female C57BL/6 mice was investigated. It was found that, treatment with CLA reduced the energy intake and effectively protected the mice from HFD-induced body weight gain, fat accumulation within the adipose tissues and liver, and impairment in energy metabolism. Further investigation revealed that CLA significantly down-regulated the expression of ORX, ORXR2, pMCH, and Gal in the hypothalamus and antagonized HFD-induced changes in the expression of UCP1, Pgc-1α, Tfam, and Mfn1 in the inguinal white adipose tissue (iWAT); Caveolin-1, MT and UCP3 in the perigonadal white adipose tissue (pWAT); and Pdhb, IRS2, Mttp, Hadhb, and Cpt1b in the liver. CLA also protected the pWAT and liver from HFD-induced mitochondrial damage. However, neither HFD nor CLA showed an effect on the mass of brown adipose tissue (BAT) or the expression of UCP1 in the BAT. In summary, our findings suggest that CLA is a potential drug candidate for preventing diet-induced obesity, at least in females. CLA works most likely by suppressing the hypothalamic expression of orexigenic genes, which leads to reduced energy intake, and subsequently, reduced fat accumulation, thereby protecting the adipose tissues and the liver from lipid-induced mitochondrial dysfunction.

scholarly journals Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice

2021 ◽  
pp. 1234-1240
Author(s):  
Mas Rizky A. A. Syamsunarno ◽  
Fenty Alia ◽  
Neni Anggraeni ◽  
Vanessa Ayu Sumirat ◽  
Suhendra Praptama ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bioactive Compounds ◽  
High Fat Diet ◽  
Moringa Oleifera ◽  
Chow Diet ◽  
High Fat ◽  
Bone Morphogenetic Protein 7 ◽  
Protein Levels ◽  
Morphogenetic Protein ◽  
Brown Adipose

Background and Aim: Brown adipose tissue's (BAT) ability to increase energy expenditure has become a new focus in obesity research. The amount and activity of BAT are inversely correlated with body-mass index and body fat percentage. Bone morphogenetic protein 7 (BMP7) plays a role in the differentiation and development of BAT, which can be increased by bioactive compounds from several medicinal plants. Moringa oleifera (MO) leaves are rich with vitamin, minerals, and bioactive compounds and have been used for treating obesity-related diseases in the past. The aim of this study was to explore the potency of MO leaf extract (MOLE) to modulate BAT differentiation in mice fed a high-fat diet (HFD). Materials and Methods: Twenty-four, 5-week-old male Deutsche Denken Yoken mice (Mus musculus) were randomly divided into four groups: The normal chow diet group was fed a normal diet, the HFD group was fed a HFD, the HFD+MOLE1, and the HFD+MOLE2 groups were fed HFD and MOLE in a dose of 280 and 560 mg/kg body weight (BW)/day, respectively. The experiment was performed for 7 weeks. At the end of the experiment, histological analysis was performed on the interscapular BAT, and blood was drawn for BMP7 protein levels. Results: After 7 weeks, BAT weight in the HFD group was nearly twice in the weight of the HFD+MOLE1 group (125±13.78 mg vs. 75±13.78 mg; p<0.001). There was also a significant increase in BAT cell density in the HFD+MOLE1 group. BMP7 serum protein levels were significantly higher in the HFD+MOLE1 group compared to the HFD group. Conclusion: The administration of MOLE in a dose of 280 mg/kg BW/day in HFD-mice induces BAT differentiation and proliferation by upregulating BMP7 protein levels.

scholarly journals Deletion of PPAR  in adipose tissues of mice protects against high fat diet-induced obesity and insulin resistance

2005 ◽  
Vol 102 (17) ◽  
pp. 6207-6212 ◽  
Author(s):  
J. R. Jones ◽  
C. Barrick ◽  
K.-A. Kim ◽  
J. Lindner ◽  
B. Blondeau ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
High Fat Diet ◽  
High Fat ◽  
Adipose Tissues ◽  
Diet Induced Obesity

Glucagon under high fat diet induced obesity

2007 ◽  
Vol 115 (S 1) ◽  
Author(s):  
LC Bollheimer ◽  
H Wobser ◽  
CE Wrede ◽  
A Schäffler ◽  
J Schölmerich ◽  
...  
Keyword(s):  
High Fat Diet ◽  
High Fat ◽  
Diet Induced Obesity
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