A cell preparation system for realising automatic zebra fish cell injection

2012 ◽  
Vol 2 (2) ◽  
pp. 93
Author(s):  
Cong Lu ◽  
James K. Mills
Keyword(s):  
Fish Cell ◽  
Zebra Fish ◽  
Cell Preparation ◽  
Cell Injection ◽  
A Cell

Fish cell culture: Characteristics of a cell line from the silver perch,Bairdiella chrysura

In Vitro ◽  
1977 ◽  
Vol 13 (6) ◽  
pp. 389-397 ◽  
Author(s):  
J. H. Wharton ◽  
R. D. Ellender ◽  
B. L. Middlebrooks ◽  
P. K. Stocks ◽  
Adrian R. Lawler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
Culture Characteristics ◽  
A Cell

A cell preparation method allowing subcellular localization of cholesterol and phosphocholine with imaging TOF-SIMS

2003 ◽  
Vol 30 (1-2) ◽  
pp. 87-92 ◽  
Author(s):  
Håkan Nygren ◽  
Cecilia Eriksson ◽  
Per Malmberg ◽  
Herman Sahlin ◽  
Lennart Carlsson ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Preparation Method ◽  
Cell Preparation ◽  
Tof Sims ◽  
A Cell

scholarly journals Immobilization of fish chromatophores for use as a micro-biosensor for biological toxins

2003 ◽  
Vol 57 (12) ◽  
pp. 605-610 ◽  
Author(s):  
Ljiljana Mojovic ◽  
Goran Jovanovic
Keyword(s):  
Cell Attachment ◽  
Cell Immobilization ◽  
Betta Splendens ◽  
Microbial Pathogens ◽  
Fish Cell ◽  
First Order Kinetics ◽  
Microcarrier Bead ◽  
Color Response ◽  
A Cell ◽  
Selection Of

Chromatophores isolated from the Siamese fighting fish, Betta splendens represent a class of living cells that provide a vivid color response to microbial pathogens and environmental toxins. The selection of the most appropriate microcarrier and the development of the optimal technique for the chromatophore immobilization in order to enable directed transport of the sensor cells throughout microchannels of the biosensor, as well to preserve the cell survival and its functionality was studied. Microcarriers derived from glass, polystyrene and gelatin (collagen) were tested as substrates for chromatophore attachement. Gelatin microcarriers were found to be the most suitable, due to high attachment efficiency (95% of attached cells), preservation of the cell viability and enhanced cell sensitivity. The optimum conditions for fish cell immobilization on collagen microcarriers were determined based on the cell-to-microcarrier bead ratio and the pH of the solution. The rate of cell attachment to the gelatin microcarrier followed first-order kinetics. Pretreatment of the gelatin beads with fibronectin, known as a cell attachment-promoting agent, resulted in a 10% higher attachment rate constant (k).

The transformation of arsenicals by Candida humicola

1979 ◽  
Vol 25 (10) ◽  
pp. 1201-1205 ◽  
Author(s):  
William R. Cullen ◽  
Barry C. McBride ◽  
A. Wendy Pickett
Keyword(s):  
Analytical Procedure ◽  
Biological Synthesis ◽  
Cell Preparation ◽  
Detection And Identification ◽  
Unknown Species ◽  
Cell Homogenate ◽  
A Cell ◽  
Trimethylarsine Oxide

An analytical procedure for the separation, detection, and identification of some of the compounds produced by a cell preparation of the fungus Candida humicola from 74As-arsenate, [14C]-methylarsonate, and [14C]-dimethylarsinate has been devised and tested. It has been possible to detect five distinct 74As-containing compounds following the incubation of 74As-arsenate with a broken cell homogenate, three of which have been identified as arsenite, methylarsonate, and dimethylarsinate. With [14C]methylarsonate as substrate, [14C]dimethylarsinate and [14C]trimethylarsine oxide are produced. Products from [14C|dimethylarsinate include [14C]methylarsonate and [14C]trimethylarsine oxide as well as an unknown species. The absence of any transformation when buffer replaces the cell preparation implicates these various compounds as intermediates in a biological synthesis of trimethylarsine.

Fish Cell Culture: Properties of a Cell Line from the Sheepshead, Archosargus probatocephalus

1978 ◽  
Vol 35 (4) ◽  
pp. 470-473 ◽  
Author(s):  
W. M. Law ◽  
R. D. Ellender ◽  
J. H. Wharton ◽  
B. L. Middlebrooks
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Chloride Concentration ◽  
Antibody Test ◽  
Lactic Dehydrogenase ◽  
Fish Cell ◽  
Cytotoxic Antibody ◽  
Fish Cell Culture ◽  
A Cell ◽  
Key Words Fish

A cell line designated SHF-1 was established from fin tissue of the sheepshead, Archosargus probatocephalus. Cells were fibroblastlike and grew best at 26 °C in Leibovitz (L-15) medium containing serum supplements and a final sodium chloride concentration of 0.150 M. The 2N number of primary sheepshead cells was 48 but the SHF-1 line at passage levels above 11 was heteroploid. SHF-1 cells did not replicate any of seven viruses tested. Species confirmation of SHF-1 was accomplished by the cytotoxic antibody test and was attempted with isoelectric focusing of lactic dehydrogenase isoenzymes. Plating efficiency and freeze viability studies were also performed. Key words: fish cell culture, sheepshead, Archosargus probatocephalus, microbiology

Hybrid force and position control of a conducting tri-layer electro-active polymer actuator

2016 ◽  
Vol 39 (3) ◽  
pp. 288-296 ◽  
Author(s):  
M Y Coskun ◽  
C Sancak ◽  
M Itik ◽  
G Alici
Keyword(s):  
Position Control ◽  
Control Strategies ◽  
Point Of View ◽  
Smith Predictor ◽  
Electroactive Polymer ◽  
Cell Injection ◽  
Injection Process ◽  
Polymer Actuator ◽  
A Cell ◽  
Position Control System

In this study, the displacement and blocking force of the tip point of a cantilevered electro-active polymer (EAP) actuator has been controlled for a cell injection process which consists of approaching, interacting and leaving steps. A vision-based system is used to acquire the tip displacement data for identifying a transfer function model of the actuator and its position control. Discrete time Proportional-Integral controllers are used to control the position and blocking force. A Smith Predictor is utilized in the vision-based position control system to compensate for the time delay due to image processing. Experimental position and blocking force results prove that the proposed control strategies are effective enough to guide the actuator to undertake the cell injection process. This study contributes to the previously published work from the point of view of simultaneously controlling the position and blocking force of the electroactive polymer actuators and widening their application areas.

PURIFICATION AND CHARACTERIZATION OF LEYDIG CELLS FROM RAT TESTES

1976 ◽  
Vol 70 (3) ◽  
pp. 345-359 ◽  
Author(s):  
F. H. A. JANSZEN ◽  
B. A. COOKE ◽  
M. J. A. VAN DRIEL ◽  
H. J. VAN DER MOLEN
Keyword(s):  
Incubation Period ◽  
Leydig Cell ◽  
Leydig Cells ◽  
Cell Preparation ◽  
Collagenase Treatment ◽  
A Cell ◽  
Dextran Solution ◽  
Isopycnic Centrifugation ◽  
Original Crude

SUMMARY An LH-responsive Leydig cell preparation (containing 6 ± 2% Leydig cells) was obtained by collagenase treatment of rat testis. Centrifugation of this cell preparation through a 13% Ficoll solution for 10 min at 1500 g resulted in a four times purification of the Leydig cells, with a concomitant increase in steroidogenic activity. Addition of 0·2% albumin to the 13% Ficoll solution, adjusted to 280 mosmol/l, resulted in a further twofold purification of the Leydig cells paralleled by a twofold increase in steroidogenic activity. Centrifugation of these Ficoll-albumin-purified Leydig cells through a 6% dextran solution for 2 min at 100 g resulted in a further 1·7 times purification of the Leydig cells. A combination of the two centrifugation steps resulted in a 12·5 times purification of Leydig cells compared with the original crude cell suspension, while an increase in steroidogenic activity of 22·5 times was obtained. This final cell preparation contained 59 ± 17% Leydig cells (mean ± s.d., n = 6). The recovery of Leydig cells was 29%. Collagenase treatment of testes deficient in spermatogenesis resulted in a cell preparation with the same steroidogenic activity as Ficoll-purified cells from normal testes. Centrifugation of these cells through a 13% Ficoll solution gave only a limited increase in the steroidogenic activity. Isopycnic centrifugation of the crude cell preparation on a discontinuous Ficoll metrizoate gradient resulted in two discrete peaks of Leydig cells, one peak at a density of 1·039–1·055 g/ml and one at a density of 1·068–1·088 g/ml. Both types of cells produced testosterone. In the presence of LH, cyclic AMP production in both types of Leydig cells increased, but testosterone production was only increased by LH in the 'denser' Leydig cells and not in the 'light' Leydig cells. No difference in sensitivity to LH could be observed between the Leydig cell preparations of different purity. Using a 60 min pre-incubation period the highest testosterone response was obtained with 100–1000 ng LH/ml. The same maximum testosterone response was obtained with 10–100 ng LH/ml when the pre-incubation period was omitted.

scholarly journals Transcription-dependent induction of G1 phase during the zebra fish midblastula transition.

1997 ◽  
Vol 17 (2) ◽  
pp. 529-536 ◽  
Author(s):  
E Zamir ◽  
Z Kam ◽  
A Yarden
Keyword(s):  
Cell Cycle ◽  
Early Development ◽  
Actinomycin D ◽  
Flow Cytometric Analysis ◽  
G1 Phase ◽  
Fish Cell ◽  
Zebra Fish ◽  
Midblastula Transition ◽  
Cell Cycles ◽  
Zygotic Transcription

The early development of the zebra fish (Danio rerio) embryo is characterized by a series of rapid and synchronous cell cycles with no detectable transcription. This period is followed by the midblastula transition (MBT), during which the cell cycle gradually lengthens, cell synchrony is lost, and zygotic transcription is initially detected. In this work, we examined the changes in the pattern of the cell cycle during MBT in zebra fish and whether these changes are dependent on the initiation of zygotic transcription. To characterize the pattern of the early zebra fish cell cycles, the embryonic DNA content was determined by flow cytometric analysis. We found that G1 phase is below detection levels during the first 10 cleavages and can be initially detected at the onset of MBT. Inhibition of zygotic transcription, by microinjection of actinomycin D, abolished the appearance of G1 phase at MBT. Premature activation of zygotic transcription, by microinjection of nonspecific DNA, induced G1 phase before the onset of MBT, while coinjection of actinomycin D and nonspecific DNA abolished this early appearance of G1 phase. We therefore suggest that during the early development of the zebra fish embryo, G1 phase appears at the onset of MBT and that the activation of transcription at MBT is essential and sufficient for the G1-phase induction.

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