scholarly journals Genetic diversity and population structure of Fusarium oxysporum f. sp. cepae, the causal agent of fusarium basal plate rot on onion, using RAPD markers

2010 ◽  
Vol 16 (3) ◽  
pp. 139-149
Author(s):  
BAYRAKTAR Harun
2012 ◽  
Vol 60 (3) ◽  
pp. 893-903 ◽  
Author(s):  
Sundan Suresh ◽  
Jong-Wook Chung ◽  
Jung-Sook Sung ◽  
Gyu-Taek Cho ◽  
Jong-Hyun Park ◽  
...  

2020 ◽  
Vol 10 (9) ◽  
pp. 3261-3269
Author(s):  
Hannah C Halpern ◽  
Peng Qi ◽  
Robert C Kemerait ◽  
Marin T Brewer

Abstract To better understand the evolution of virulence we are interested in identifying the genetic basis of this trait in pathogenic fungi and in developing tools for the rapid characterization of variation in virulence among populations associated with epidemics. Fusarium oxysporum f. sp. vasinfectum (FOV) is a haploid fungus that causes devastating outbreaks of Fusarium wilt of cotton wherever it is grown. In the United States, six nominal races and eleven genotypes of FOV have been characterized based on the translation elongation factor (EF-1α) gene and intergenic spacer region (IGS), but it is unclear how race or genotype based on these regions relates to population structure or virulence. We used genotyping-by-sequencing to identify SNPs and determine genetic diversity and population structure among 86 diverse FOV isolates. Six individuals of Fusarium oxysporum closely related to FOV were genotyped and included in some analyses. Between 193 and 354 SNPs were identified and included in the analyses depending on the pipeline and filtering criteria used. Phylogenetic trees, minimum spanning networks (MSNs), principal components analysis (PCA), and discriminant analysis of principal components (DAPC) demonstrated that races and genotypes of FOV are generally not structured by EF-1α genotype, nor are they monophyletic groups with the exception of race 4 isolates, which are distinct. Furthermore, DAPC identified between 11 and 14 genetically distinct clusters of FOV, whereas only eight EF-1α genotypes were represented among isolates; suggesting that FOV, especially isolates within the widely distributed and common race 1 genotype, is more genetically diverse than currently recognized.


2014 ◽  
Vol 36 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Can-Wei Shu ◽  
Cheng-Jia Zou ◽  
Jie-Ling Chen ◽  
Fang Tang ◽  
Run-Hua Yi ◽  
...  

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1125
Author(s):  
Carla L. Saldaña ◽  
Johan D. Cancan ◽  
Wilbert Cruz ◽  
Mirian Y. Correa ◽  
Miriam Ramos ◽  
...  

Capirona (Calycophyllum spruceanum Benth.) is a tree species of commercial importance widely distributed in South American forests that is traditionally used for its medicinal properties and wood quality. Studies on this tree species have been focused mainly on wood properties, propagation, and growth. However, genetic studies on capirona have been very limited to date. Currently, it is possible to explore genetic diversity and population structure in a fast and reliable manner by using molecular markers. We here used 10 random amplified polymorphic DNA (RAPD) markers to analyze the genetic diversity and population structure of 59 samples of capirona that were sampled from four provinces located in the eastern region of the Peruvian amazon. A total of 186 bands were manually scored, generating a 59 × 186 presence/absence matrix. A dendrogram was generated using the UPGMA clustering algorithm, and, similar to the principal coordinate analysis (PCoA), it showed four groups that correspond to the geographic origin of the capirona samples (LBS, Irazola, Masisea, Iñapari). Similarly, a discriminant analysis of principal components (DAPC) and STRUCTURE analysis confirmed that capirona is grouped into four clusters. However, we also noticed that a few samples were intermingled. Genetic diversity estimation was conducted considering the four groups (populations) identified by STRUCTURE software. AMOVA revealed the greatest variation within populations (71.56%) and indicated that variability among populations is 28.44%. Population divergence (Fst) between clusters 1 and 4 revealed the highest genetic difference (0.269), and the lowest Fst was observed between clusters 3 and 4 (0.123). RAPD markers were successful and effective. However, more studies are needed, employing other molecular tools. To the best of our knowledge, this is the first investigation employing molecular markers in capirona in Peru considering its natural distribution, and as such it is hoped that this helps to pave the way towards its genetic improvement and the urgent sustainable management of forests in Peru.


2018 ◽  
Vol 75 (4) ◽  
pp. 546
Author(s):  
Chavlesh Kumar ◽  
S. K. Singh ◽  
Rakesh Singh ◽  
K. K. Pramanick ◽  
M. K. Verma ◽  
...  

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