scholarly journals A comparative study of zero-order and derivative spectrophotometric methods for the determination of rivastigmine in single dosage form

2004 ◽  
pp. 013-033
Author(s):  
S. Dermiş GÜNGÖR
Keyword(s):  
Comparative Study ◽  
Dosage Form ◽  
Zero Order ◽  
Spectrophotometric Methods ◽  
Single Dosage

scholarly journals Simple two spectrophotometric methods for estimation of Cephalexin in pure and pharmaceutical dosage form

2021 ◽  
Vol 3 (1) ◽  
pp. 37-45
Author(s):  
Neda Ibrahim Mahdi .
Keyword(s):  
Area Under Curve ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Zero Order ◽  
Maximum Absorption ◽  
Order Method ◽  
Pharmaceutical Dosage Form ◽  
Spectrophotometric Methods ◽  
Curve Method

This study describes two extraction-free and direct spectrophotometric methods for the determination of Cephalexin in a pure and pharmaceutical dosage form. In this study, a zero-order method has exhibited maximum absorption max at 263 nm, while area under curve method has calculated in the range 260-266nm. The Linearity of both methods in the range (10- 60 nm), the limit of detection (LOD) is 0.808 for Zero-order method and 0.781 for the area under curve method. These methods are successfully applied to determination of Cephalexin in a pharmaceutical dosage form.

UV SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF PALBOCICLIB IN BULK AND IN-HOUSE CAPSULE FORMULATION

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (08) ◽  
pp. 35-40
Author(s):  
Rahul D. Rathod ◽  
Anand B. Mundada ◽  
Harun M. Patel ◽  
Atul A Shirkhedkar ◽  
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Zero Order ◽  
Distilled Water ◽  
Order Method ◽  
Capsule Formulation ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Made In

Four different simple, accurate and precise UV-spectrophotometric methods have been developed for the estimation of palbociclib (PB) in bulk and in-house capsule dosage form by zero order (Method I), zero order AUC (Method II), ‘first order derivative UV-spectrophotometric (Method III), and first order AUC (Method IV) methods. The drug was dissolved in methanol (AR-Grade) and further dilution was made in double distilled water. Zero order was performed at λ max 220.00 nm of PB (Method I) and AUC was calculated between 215.40 nm - 228.20 nm wavelength (Method II). In Method-III zero-order spectra were derivatized into first-order and amplitude measured at 231.00 nm and the AUC was recorded between 224.00 nm - 240.60 nm (Method IV). PB followed linearity in the concentration range of 4.08-20.40 µg/mL with correlation coefficient (r2 )> 0.99 for PB.

scholarly journals Simultaneous spectrophotometric determination of drugs lacking peak maxima in their zero-order profiles by graphical or statistical representation of data

2018 ◽  
Vol 9 (3) ◽  
pp. 194-201
Author(s):  
Ragaa Magdy ◽  
Ahmed Hemdan ◽  
Nermine Victor Fares ◽  
Maha Farouk
Keyword(s):  
Graphical Representation ◽  
Reference Method ◽  
Zero Order ◽  
Regression Equations ◽  
Spectrophotometric Methods ◽  
Statistical Representation ◽  
Tablet Dosage ◽  
Direct Spectrophotometry ◽  
One Way Anova

Trandolapril has no sharp peak in its zero-order spectrum, therefore it is difficult to be measured by direct spectrophotometry. In this study, direct univariate spectrophotometric methods were developed and validated for determination of Trandolapril and Verapamil combination in pure and tablet dosage forms. The first method for measuring both Trandolapril and Verapamil is Absorbance Subtraction (AS), this method depends on the presence of iso-absorptive point in the zero-order curve at 217 nm. It has the advantage of measuring the concentration of both Trandolapril and Verapamil from unified regression equation at the iso-absorptive point. The second, third and fourth methods were applied on the first order spectra of the studied drugs. Second method is Derivative Subtraction (DS) for Trandolapril and Derivative subtraction followed by spectrum subtraction (DS-SS) for Verapamil. The third and fourth methods are constant value and concentration value methods. In the concentration value method, the concentration of the drugs is determined from the graphical representation without the use of regression equations. All the developed methods were validated as per International Conference on Harmonization guidelines and the results proved that the developed methods are simple, accurate, and selective. Moreover, a statistical comparison between the developed methods and a reference method was done. Also, One-way ANOVA statistical test was done between all the proposed spectrophoto-metric methods and results showed no significant differences.

scholarly journals Spectrophometric Determination of Nelfinavir Mesylate

2006 ◽  
Vol 3 (2) ◽  
pp. 78-82 ◽  
Author(s):  
K. Vanitha Prakash ◽  
Jangala Venkateswara Rao
Keyword(s):  
Ferric Chloride ◽  
Dosage Form ◽  
Potassium Ferricyanide ◽  
Reagent Blank ◽  
Spectrophotometric Methods ◽  
Bluish Green ◽  
Nelfinavir Mesylate ◽  
Bulk Drug ◽  
Tablet Dosage

Two new simple, sensitive, rapid and economical Spectrophotometric Methods (A and B) have been developed for the determination of Nelfinavir Mesylate in pharmaceutical bulk and tablet dosage form. The method A is based on the reaction of Nelfinavir with ferric chloride, potassium ferricyanide and hydrochloric acid to form a bluish green colored chromogen. The Method B is based on the formation of blood red colored chromogen with Ferric chloride and 1,10-phenanthroline. The absorbances of the chromogen were measured at their respective wavelength of maximum absorbance against the corresponding reagent blank. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate.

Spectrophotometric Methods for Simultaneous Determination of Sofosbuvir and Ledipasvir (HARVONI Tablet): Comparative Study with Two Generic Products

2017 ◽  
Vol 100 (4) ◽  
pp. 976-984 ◽  
Author(s):  
Nisreen F Abo-Talib ◽  
Mohamed R El-Ghobashy ◽  
Marwa H Tammam
Keyword(s):  
Dosage Form ◽  
Drug Dosage ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Drug Dosage Form ◽  
Generic Products ◽  
Third Derivative ◽  
Combination Pill

Abstract Sofosbuvir and ledipasvir are the first drugs in a combination pill to treat chronic hepatitis C virus. Simple, sensitive, and rapid spectrophotometric methods are presented for the determination of sofosbuvir and ledipasvir in their combined dosage form. These methods were based on direct measurement of ledipasvir at 333 nm (due to the lack of interference of sofosbuvir) over a concentration range of 4.0–14.0 µg/mL, with a mean recovery of 100.78 ± 0.64%. Sofosbuvir was determined, without prior separation, by third-derivative values at 281 nm; derivative ratio values at 265.8 nm utilizing 5.0 µg/mL ledipasvir as a divisor; the ratio difference method using values at 270 and 250 nm using 5.0 µg/mL ledipasvir as a divisor; and the ratio subtraction method using values at 261 nm. These methods were found to be linear for sofosbuvir over a concentration range of 5.0–35.0 µg/mL. The suggested methods were validated according to International Conference on Harmonization guidelines. Statistical analysis of the results showed no significant difference between the proposed methods and the manufacturer's LC method of determination with respect to accuracy and precision. These methods were used to compare the equivalence of an innovator drug dosage form and two generic drug dosage forms of the same strength.

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