scholarly journals A simple antegrade perfusion method for isolating viable single cardiomyocytes from neonatal to aged mice

2018 ◽  
Vol 6 (9) ◽  
pp. e13688 ◽  
Author(s):  
Mariko Omatsu-Kanbe ◽  
Kengo Yoshioka ◽  
Ryo Fukunaga ◽  
Hironori Sagawa ◽  
Hiroshi Matsuura
Keyword(s):  
Aged Mice ◽  
Perfusion Method

γ‐Oryzanol improves exercise endurance and muscle strength by upregulating PPARδ and ERRγ activity in aged mice

2021 ◽  
pp. 2000652
Author(s):  
Jisong Ahn ◽  
Hyo Jeong Son ◽  
Hyo Deok Seo ◽  
Tae Youl Ha ◽  
Jiyun Ahn ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Aged Mice ◽  
Exercise Endurance

scholarly journals Bcl-2-dependent autophagy disruption during aging impairs amino acid utilization that is restored by hochuekkito

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Miwa Nahata ◽  
Sachiko Mogami ◽  
Hitomi Sekine ◽  
Seiichi Iizuka ◽  
Naoto Okubo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Energy Homeostasis ◽  
Mitochondrial Dynamics ◽  
Negative Regulator ◽  
Aged Mice ◽  
Glucose Levels ◽  
Therapeutic Benefits ◽  
Age Related ◽  
Daily Food ◽  
Young Mice

AbstractChronic undernutrition contributes to the increase in frailty observed among elderly adults, which is a pressing issue in the sector of health care for older people worldwide. Autophagy, an intracellular recycling system, is closely associated with age-related pathologies. Therefore, decreased autophagy in aging could be involved in the disruption of energy homeostasis that occurs during undernutrition; however, the physiological mechanisms underlying this process remain unknown. Here, we showed that 70% daily food restriction (FR) induced fatal hypoglycemia in 23–26-month-old (aged) mice, which exhibited significantly lower hepatic autophagy than 9-week-old (young) mice. The liver expressions of Bcl-2, an autophagy-negative regulator, and Beclin1–Bcl-2 binding, were increased in aged mice compared with young mice. The autophagy inducer Tat-Beclin1 D11, not the mTOR inhibitor rapamycin, decreased the plasma levels of the glucogenic amino acid and restored the blood glucose levels in aged FR mice. Decreased liver gluconeogenesis, body temperature, physical activity, amino acid metabolism, and hepatic mitochondrial dynamics were observed in the aged FR mice. These changes were restored by treatment with hochuekkito that is a herbal formula containing several autophagy-activating ingredients. Our results indicate that Bcl-2 upregulation in the liver during the aging process disturbs autophagy activation, which increases the vulnerability to undernutrition. The promotion of liver autophagy may offer clinical therapeutic benefits to frail elderly patients.

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