scholarly journals Oligophrenin-1: the link between calcium-regulated exocytosis and compensatory endocytosis in neuroendocrine cells

2016 ◽  
Keyword(s):  
Neuroendocrine Cells ◽  
Regulated Exocytosis

Synaptic Transmission in the Immune System

e-Neuroforum ◽  
2017 ◽  
Vol 23 (4) ◽  
Author(s):  
Jens Rettig ◽  
David R. Stevens
Keyword(s):  
Nervous System ◽  
Immune System ◽  
Synaptic Transmission ◽  
Molecular Mechanisms ◽  
Secretory Granules ◽  
Neuroendocrine Cells ◽  
Regulated Exocytosis ◽  
Protein Receptor ◽  
Immunological Synapses ◽  
Immunological Diseases

AbstractThe release of neurotransmitters at synapses belongs to the most important processes in the central nervous system. In the last decades much has been learned about the molecular mechanisms which form the basis for this fundamental process. Highly regulated exocytosis, based on the SNARE (soluble N-ethylmaleimide-sensitive attachment protein receptor) complex and its regulatory molecules is the signature specialization of the nervous system and is shared by neurons and neuroendocrine cells. Cells of the immune system use a similar mechanism to release cytotoxic materials from secretory granules at contacts with virally or bacterially infected cells or cancer cells, in order to remove these threats. These contact zones have been termed immunological synapses in reference to the highly specific targeted exocytosis of effector molecules. Recent findings indicate that mutations in SNARE or SNARE-interacting proteins are the basis of a number of devastating immunological diseases. While SNARE complexes are ubiquitous and mediate a wide variety of membrane fusion events it is surprising that in many cases the SNARE proteins involved in immunological synapses are the same molecules which mediate regulated exocytosis of transmitters and hormones in neurons and neuroendocrine cells. These similarities raise the possibility that results obtained at immunological synapses may be applicable, in particular in the area of presynaptic function, to neuronal synapses. Since immunological synapses (IS) are assembled and disassembled in about a half an hour, the use of immune cells isolated from human blood allows not only the study of the molecular mechanisms of synaptic transmission in human cells, but is particularly suited to the examination of the assembly and disassembly of these “synapses” via live imaging. In this overview we discuss areas of similarity between synapses of the nervous and immune systems and in the process will refer to results of our experiments of the last few years.

Regulation of exocytosis by protein kinase C

2005 ◽  
Vol 33 (6) ◽  
pp. 1341-1344 ◽  
Author(s):  
A. Morgan ◽  
R.D. Burgoyne ◽  
J.W. Barclay ◽  
T.J. Craig ◽  
G.R. Prescott ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Cell Types ◽  
Neuroendocrine Cells ◽  
Protein Targets ◽  
Site Specific ◽  
Regulated Exocytosis ◽  
Kinase C ◽  
Pkc Protein Kinase C

PKC (protein kinase C) has been known for many years to modulate regulated exocytosis in a wide variety of cell types. In neurons and neuroendocrine cells, PKC regulates several different stages of the exocytotic process, suggesting that these multiple actions of PKC are mediated by phosphorylation of distinct protein targets. In recent years, a variety of exocytotic proteins have been identified as PKC substrates, the best characterized of which are SNAP-25 (25 kDa synaptosome-associated protein) and Munc18. In the present study, we review recent evidence suggesting that site-specific phosphorylation of SNAP-25 and Munc18 by PKC regulates distinct stages of exocytosis.

scholarly journals ARF6 Regulates the Synthesis of Fusogenic Lipids for Calcium-regulated Exocytosis in Neuroendocrine Cells

2009 ◽  
Vol 284 (8) ◽  
pp. 4836-4845 ◽  
Author(s):  
Aurélie Béglé ◽  
Petra Tryoen-Tóth ◽  
Jean de Barry ◽  
Marie-France Bader ◽  
Nicolas Vitale
Keyword(s):  
Neuroendocrine Cells ◽  
Regulated Exocytosis

scholarly journals Regulated Exocytosis in Neuroendocrine Cells: A Role for Subplasmalemmal Cdc42/N-WASP-induced Actin Filaments

2004 ◽  
Vol 15 (2) ◽  
pp. 520-531 ◽  
Author(s):  
Stéphane Gasman ◽  
Sylvette Chasserot-Golaz ◽  
Magali Malacombe ◽  
Michael Way ◽  
Marie-France Bader
Keyword(s):  
Plasma Membrane ◽  
Membrane Trafficking ◽  
Actin Polymerization ◽  
Cell Activation ◽  
Secretory Granules ◽  
Small Gtpases ◽  
Neuroendocrine Cells ◽  
Actin Dynamics ◽  
Cell Periphery ◽  
Regulated Exocytosis

In neuroendocrine cells, actin reorganization is a prerequisite for regulated exocytosis. Small GTPases, Rho proteins, represent potential candidates coupling actin dynamics to membrane trafficking events. We previously reported that Cdc42 plays an active role in regulated exocytosis in chromaffin cells. The aim of the present work was to dissect the molecular effector pathway integrating Cdc42 to the actin architecture required for the secretory reaction in neuroendocrine cells. Using PC12 cells as a secretory model, we show that Cdc42 is activated at the plasma membrane during exocytosis. Expression of the constitutively active Cdc42L61 mutant increases the secretory response, recruits neural Wiskott-Aldrich syndrome protein (N-WASP), and enhances actin polymerization in the subplasmalemmal region. Moreover, expression of N-WASP stimulates secretion by a mechanism dependent on its ability to induce actin polymerization at the cell periphery. Finally, we observed that actin-related protein-2/3 (Arp2/3) is associated with secretory granules and that it accompanies granules to the docking sites at the plasma membrane upon cell activation. Our results demonstrate for the first time that secretagogue-evoked stimulation induces the sequential ordering of Cdc42, N-WASP, and Arp2/3 at the interface between granules and the plasma membrane, thereby providing an actin structure that makes the exocytotic machinery more efficient.

scholarly journals Mono- and Poly-unsaturated Phosphatidic Acid Regulate Distinct Steps of Regulated Exocytosis in Neuroendocrine Cells

Cell Reports ◽  
2020 ◽  
Vol 32 (7) ◽  
pp. 108026 ◽  
Author(s):  
Emeline Tanguy ◽  
Pierre Costé de Bagneaux ◽  
Nawal Kassas ◽  
Mohamed-Raafet Ammar ◽  
Qili Wang ◽  
...  
Keyword(s):  
Phosphatidic Acid ◽  
Neuroendocrine Cells ◽  
Regulated Exocytosis

scholarly journals Phosphorylation cycling of Annexin A2 Tyr23 is critical for calcium-regulated exocytosis in neuroendocrine cells

2019 ◽  
Vol 1866 (7) ◽  
pp. 1207-1217 ◽  
Author(s):  
Marion Gabel ◽  
Franck Delavoie ◽  
Cathy Royer ◽  
Tam Tahouly ◽  
Stéphane Gasman ◽  
...  
Keyword(s):  
Annexin A2 ◽  
Neuroendocrine Cells ◽  
Regulated Exocytosis

Calcium-regulated Exocytosis in Neuroendocrine Cells: Intersectin-1L Stimulates Actin Polymerization and Exocytosis by Activating Cdc42

2009 ◽  
Vol 1152 (1) ◽  
pp. 209-214 ◽  
Author(s):  
Fanny Momboisse ◽  
Stéphane Ory ◽  
Valerie Calco ◽  
Magali Malacombe ◽  
Marie-France Bader ◽  
...  
Keyword(s):  
Actin Polymerization ◽  
Neuroendocrine Cells ◽  
Regulated Exocytosis

Gliotransmission: focus on exocytotic release of L-glutamate and D-serine from astrocytes

2013 ◽  
Vol 41 (6) ◽  
pp. 1557-1561 ◽  
Author(s):  
Magalie Martineau
Keyword(s):  
Amino Acids ◽  
Plasma Membrane ◽  
Synaptic Plasticity ◽  
Membrane Proteins ◽  
Cognitive Functions ◽  
Neurological Disorders ◽  
Excitatory Amino Acids ◽  
Neuroendocrine Cells ◽  
Present Review ◽  
Regulated Exocytosis

The release of neuromodulators, called gliotransmitters, by astrocytes is proposed to modulate neurotransmission and synaptic plasticity, and thereby cognitive functions; but they are also proposed to have a role in diverse neurological disorders. Two main routes have been proposed to ensure gliotransmitter release: non-exocytotic release from cytosolic pools through plasma membrane proteins, and Ca2+-regulated exocytosis through the fusion of gliotransmitter-storing secretory organelles. Regulated Ca2+-dependent glial exocytosis has received much attention and is appealing since its existence endows astrocytes with some of the basic properties thought to be exclusive to neurons and neuroendocrine cells. The present review summarizes recent findings regarding the exocytotic mechanisms underlying the release of two excitatory amino acids, L-glutamate and D-serine.

scholarly journals Mono- and Polyunsaturated Phosphatidic Acid Regulate Distinct Steps of Regulated Exocytosis in Neuroendocrine Cells

2020 ◽  
Author(s):  
Emeline Tanguy ◽  
Pierre Costé de Bagneaux ◽  
Nawal Kassas ◽  
Mohamed-Raafet Ammar ◽  
Qili Wang ◽  
...  
Keyword(s):  
Phosphatidic Acid ◽  
Neuroendocrine Cells ◽  
Regulated Exocytosis

scholarly journals A Direct Inhibitory Role for the Rab3-specific Effector, Noc2, in Ca2+-regulated Exocytosis in Neuroendocrine Cells

2000 ◽  
Vol 276 (13) ◽  
pp. 9726-9732 ◽  
Author(s):  
Lee P. Haynes ◽  
Gareth J. O. Evans ◽  
Alan Morgan ◽  
Robert D. Burgoyne
Keyword(s):  
Neuroendocrine Cells ◽  
Regulated Exocytosis ◽  
Inhibitory Role ◽  
Specific Effector
Sign in / Sign up

Export Citation Format

Share Document