scholarly journals Thermal Behavior of Bio-related Material Gel in Low Water Content

2009 ◽  
Vol 34 (3) ◽  
pp. 485-488
Author(s):  
Atsushi Nakamura ◽  
Ken-ichi Hirakawa ◽  
Kazuhiro Hara ◽  
Nobuyasu Hiramatsu
Keyword(s):  
Thermal Behavior ◽  
Water Content ◽  
Related Material

Effect of Water Content on Thermal Behavior of Freeze-Dried Soy Whey and Their Isolated Proteins

2011 ◽  
Vol 59 (8) ◽  
pp. 3950-3956 ◽  
Author(s):  
Pablo A. Sobral ◽  
Gonzalo G. Palazolo ◽  
Jorge R. Wagner
Keyword(s):  
Thermal Behavior ◽  
Water Content ◽  
Effect Of Water ◽  
Freeze Dried

Variation of Water Content and Thermal Behavior of Talc Upon Grinding: Effect of Repeated Slip on Fault Weakening

2019 ◽  
Vol 32 (3) ◽  
pp. 201-211 ◽  
Author(s):  
Min Sik Kim ◽  
◽  
Jin Woo Kim ◽  
Chang Du Kang ◽  
Byung Dal So ◽  
...  
Keyword(s):  
Thermal Behavior ◽  
Water Content

Thermal behavior of ferric selenite hydrates (Fe2(SeO3)3·3H2O, Fe2(SeO3)3·5H2O) and the water content in the natural ferric selenite mandarinoite

Geochemistry ◽  
2018 ◽  
Vol 78 (2) ◽  
pp. 228-240 ◽  
Author(s):  
Astrid Holzheid ◽  
Marina V. Charykova ◽  
Vladimir G. Krivovichev ◽  
Brendan Ledwig ◽  
Elena L. Fokina ◽  
...  
Keyword(s):  
Thermal Behavior ◽  
Water Content

scholarly journals Thermal Behavior of Albumin Gel in Low Water Content

2010 ◽  
Vol 35 (4) ◽  
pp. 869-872
Author(s):  
Atsushi Nakamura ◽  
Kazuhiro Hara ◽  
Nobuyasu Hiramatsu
Keyword(s):  
Thermal Behavior ◽  
Water Content

Characterization of frozen hydrated biological specimens by low-loss EELS

1992 ◽  
Vol 50 (2) ◽  
pp. 1572-1573
Author(s):  
Songquan Sun ◽  
Richard D. Leapman
Keyword(s):  
Water Content ◽  
Direct Method ◽  
Internal Standard ◽  
Dry Weight ◽  
Dark Field ◽  
Protein Solutions ◽  
Subcellular Compartment ◽  
Differential Shrinkage ◽  
Electron Energy Loss

Analyses of ultrathin cryosections are generally performed after freeze-drying because the presence of water renders the specimens highly susceptible to radiation damage. The water content of a subcellular compartment is an important quantity that must be known, for example, to convert the dry weight concentrations of ions to the physiologically more relevant molar concentrations. Water content can be determined indirectly from dark-field mass measurements provided that there is no differential shrinkage between compartments and that there exists a suitable internal standard. The potential advantage of a more direct method for measuring water has led us to explore the use of electron energy loss spectroscopy (EELS) for characterizing biological specimens in their frozen hydrated state.We have obtained preliminary EELS measurements from pure amorphous ice and from cryosectioned frozen protein solutions. The specimens were cryotransfered into a VG-HB501 field-emission STEM equipped with a 666 Gatan parallel-detection spectrometer and analyzed at approximately −160 C.

STEM measurement of subcellular water distributions

1993 ◽  
Vol 51 ◽  
pp. 568-569
Author(s):  
R.D. Leapman ◽  
S.Q. Sun ◽  
S-L. Shi ◽  
R.A. Buchanan ◽  
S.B. Andrews
Keyword(s):  
Water Content ◽  
Internal Standard ◽  
Dry Weight ◽  
Dry Mass ◽  
Scanning Transmission Electron Microscope ◽  
Dark Field ◽  
Bulk Water ◽  
Inelastic Electron ◽  
Scanning Transmission ◽  
Annular Dark Field

Recent advances in rapid-freezing and cryosectioning techniques coupled with use of the quantitative signals available in the scanning transmission electron microscope (STEM) can provide us with new methods for determining the water distributions of subcellular compartments. The water content is an important physiological quantity that reflects how fluid and electrolytes are regulated in the cell; it is also required to convert dry weight concentrations of ions obtained from x-ray microanalysis into the more relevant molar ionic concentrations. Here we compare the information about water concentrations from both elastic (annular dark-field) and inelastic (electron energy loss) scattering measurements.In order to utilize the elastic signal it is first necessary to increase contrast by removing the water from the cryosection. After dehydration the tissue can be digitally imaged under low-dose conditions, in the same way that STEM mass mapping of macromolecules is performed. The resulting pixel intensities are then converted into dry mass fractions by using an internal standard, e.g., the mean intensity of the whole image may be taken as representative of the bulk water content of the tissue.

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