scholarly journals Comparative Study of Different Methods to Determine the Role of Reactive Oxygen Species Induced by Zinc Oxide Nanoparticles

2015 ◽  
Vol 3 (2) ◽  
pp. 30-34 ◽  
Author(s):  
Nigar Najim ◽  
Keyword(s):  
Reactive Oxygen Species ◽  
Zinc Oxide ◽  
Comparative Study ◽  
Zinc Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Oxide Nanoparticles ◽  
Oxygen Species

scholarly journals Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles

2021 ◽  
Vol 22 (17) ◽  
pp. 9529
Author(s):  
Anna Król-Górniak ◽  
Katarzyna Rafińska ◽  
Fernanda Monedeiro ◽  
Paweł Pomastowski ◽  
Bogusław Buszewski
Keyword(s):  
Reactive Oxygen Species ◽  
Methylene Blue ◽  
Zinc Oxide ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Uv Light ◽  
Reactive Oxygen ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Zno Nps

In this paper, a study of the cytotoxicity of bare and functionalized zinc oxide nanoparticles (ZnO NPs) is presented. The functionalized ZnO NPs were obtained by various types of biological methods including microbiological (intra- and extracellular with Lactobacillus paracasei strain), phytochemical (Medicago sativa plant extract) and biochemical (ovalbumin from egg white protein) synthesis. As a control, the bare ZnO NPs gained by chemical synthesis (commercially available) were tested. The cytotoxicity was measured through the use of (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye as well as lactate dehydrogenase (LDH) assays against murine fibroblast L929 and Caco-2 cell lines. As a complementary method, scanning electron microscopy (SEM) was performed to assess the morphology of the tested cells after treatment with ZnO NPs. The microscopic data confirmed the occurrence of apoptotic blebbing and loss of membrane permeability after the administration of all ZnO NPs. The reactive oxygen species (ROS) concentration during the cell lines’ exposure to ZnO NPs was measured fluorometrically. Additionally, the photocatalytic degradation of methylene blue (MB) dye in the different light conditions, as well as the antioxidant activity of bare and functionalized ZnO NPs, is also reported. The addition of all types of tested ZnO NPs to methylene blue resulted in enhanced rates of photo-degradation in the presence of both types of irradiation, but the application of UV light resulted in higher photocatalytic activity of ZnO NPs. Furthermore, bare (chemically synthetized) NPs have been recognized as the strongest photocatalysts. In the context of the obtained results, a mechanism underlying the toxicity of bio-ZnO NPs, including (a) the generation of reactive oxygen species and (b) the induction of apoptosis, is proposed.

scholarly journals Reactive Oxygen Species Mediated Bacterial Biofilm Inhibition via Zinc Oxide Nanoparticles and Their Statistical Determination

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e111289 ◽  
Author(s):  
Sourabh Dwivedi ◽  
Rizwan Wahab ◽  
Farheen Khan ◽  
Yogendra K. Mishra ◽  
Javed Musarrat ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Bacterial Biofilm ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Biofilm Inhibition ◽  
Statistical Determination
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