scholarly journals Arthrospira platensis Mutants Containing High Lipid Content by Electron Beam Irradiation and Analysis of Its Fatty Acid Composition

2013 ◽  
Vol 24 (6) ◽  
pp. 628-632 ◽  
Author(s):  
Soo-Jeong Choi ◽  
Young-Hwa Kim ◽  
Andre Kim ◽  
Jae-Hwa Lee
2018 ◽  
Vol 10 (2) ◽  
pp. 388-395
Author(s):  
Windra Yuniarsih ◽  
Heli Siti Halimatul ◽  
Asri Peni Wulandari

Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.


2021 ◽  
Vol 98 ◽  
pp. 103161
Author(s):  
Xiaohu Luo ◽  
Zhihong Du ◽  
Kai Yang ◽  
Jing Wang ◽  
Jing Zhou ◽  
...  

Copeia ◽  
1999 ◽  
Vol 1999 (3) ◽  
pp. 766 ◽  
Author(s):  
Ileana E. Clavijo ◽  
Carolyn S. Dunn ◽  
Pamela J. Seaton

2009 ◽  
Vol 86 (12) ◽  
pp. 1155-1161 ◽  
Author(s):  
Shu Cheng Liu ◽  
De Tao Li ◽  
Peng Zhi Hong ◽  
Chao Hua Zhang ◽  
Hong Wu Ji ◽  
...  

2010 ◽  
Vol 23 (1) ◽  
pp. 3-17 ◽  
Author(s):  
P. Mayzaud ◽  
S. Lacombre ◽  
M. Boutoute

AbstractChanges in adult weight, lipid and fatty acid composition per lipid class were studied over a complete seasonal cycle in Drepanopus pectinatus Brady. This copepod displayed a strong seasonality in adult individual weight and total lipid content. Stages C3 to adult from a summer cohort were compared in terms of lipid and fatty acid structure. Changes in lipid content were related to wax esters and polar lipids (PL) while triacylglycerols (TAG) and cholesterol remained minor constituents except at the end of winter. Changes in fatty acid composition of different lipid classes showed that food limitation in winter was high enough to affect 22:6n-3 (DHA) content in PL, and provoke accumulation of maximal percentages of bacterial marker as branched acids. Composition of TAG followed closely the changes in trophic interactions as diatom and flagellate markers dominated in spring and summer while bacterial and detritus particle markers dominated in late winter. The composition of wax esters followed the same seasonal succession but with a lower level of turn over time and shifted characteristics (bacterial markers) from winter to spring individuals when compared to TAG. Ontogenetic changes showed high triacylglycerols content in younger C3 stages and increasing wax esters with increasing developmental stages. Fatty acid composition of PL showed low percentages of EPA and DHA in stage C3, and recovery of high levels of DHA for stage C4 or 20:5n-3 (EPA) at stage C5. The same trend was observed for 18:4n-3. The reverse pattern was noted for the n-6 polyunsaturated acids (PUFA) suggesting a greater requirement in younger stages. Fatty acid partitioning between neutral and PL suggested essential fatty acids selective incorporation from neutral classes into membrane lipids.


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