scholarly journals Large-scale production of lentiviral vectors using multilayer cell factories

2018 ◽  
Vol 5 (2) ◽  
pp. 90 ◽  
Author(s):  
Nathan Rout-Pitt ◽  
Alexandra McCarron ◽  
Chantelle McIntyre ◽  
David Parsons ◽  
Martin Donnelley
Keyword(s):  
Large Scale ◽  
Lentiviral Vectors ◽  
Scale Production ◽  
Cell Factories ◽  
Large Scale Production

scholarly journals Large-Scale Production of Lentiviral Vectors: Current Perspectives and Challenges

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1051
Author(s):  
Eduardo Martínez-Molina ◽  
Carlos Chocarro-Wrona ◽  
Daniel Martínez-Moreno ◽  
Juan A. Marchal ◽  
Houria Boulaiz
Keyword(s):  
Viral Vectors ◽  
Large Scale ◽  
Lentiviral Vectors ◽  
Good Manufacturing Practice ◽  
Scale Production ◽  
Gene Therapies ◽  
Packaging Cell ◽  
Large Scale Production ◽  
Target Production

Lentiviral vectors (LVs) have gained value over recent years as gene carriers in gene therapy. These viral vectors are safer than what was previously being used for gene transfer and are capable of infecting both dividing and nondividing cells with a long-term expression. This characteristic makes LVs ideal for clinical research, as has been demonstrated with the approval of lentivirus-based gene therapies from the Food and Drug Administration and the European Agency for Medicine. A large number of functional lentiviral particles are required for clinical trials, and large-scale production has been challenging. Therefore, efforts are focused on solving the drawbacks associated with the production and purification of LVsunder current good manufacturing practice. In recent years, we have witnessed the development and optimization of new protocols, packaging cell lines, and culture devices that are very close to reaching the target production level. Here, we review the most recent, efficient, and promising methods for the clinical-scale production ofLVs.

scholarly journals Inducible Packaging Cells for Large-scale Production of Lentiviral Vectors in Serum-free Suspension Culture

2008 ◽  
Vol 16 (3) ◽  
pp. 500-507 ◽  
Author(s):  
Sophie Broussau ◽  
Nadine Jabbour ◽  
Guillaume Lachapelle ◽  
Yves Durocher ◽  
Rosanne Tom ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Large Scale ◽  
Lentiviral Vectors ◽  
Scale Production ◽  
Serum Free ◽  
Large Scale Production ◽  
Serum Free Suspension

scholarly journals Large-Scale Production of High-Quality Helper-Dependent Adenoviral Vectors Using Adherent Cells in Cell Factories

2010 ◽  
Vol 21 (1) ◽  
pp. 120-126 ◽  
Author(s):  
Masataka Suzuki ◽  
Racel Cela ◽  
Christian Clarke ◽  
Terry K. Bertin ◽  
Susana Mouriño ◽  
...  
Keyword(s):  
Large Scale ◽  
Adenoviral Vectors ◽  
Adherent Cells ◽  
Scale Production ◽  
High Quality ◽  
Cell Factories ◽  
Large Scale Production

scholarly journals 708. Process Development of Lentiviral Vectors for Large Scale Production Using a HEK293 Producer Cell Line

2016 ◽  
Vol 24 ◽  
pp. S279-S280
Author(s):  
Alexandre Audy ◽  
Marc-André Robert ◽  
Parminder Chahal ◽  
Rénald Gilbert ◽  
Bruno Gaillet
Keyword(s):  
Cell Line ◽  
Large Scale ◽  
Process Development ◽  
Lentiviral Vectors ◽  
Scale Production ◽  
Producer Cell Line ◽  
Large Scale Production ◽  
Producer Cell

Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo

2003 ◽  
Vol 12 (3) ◽  
pp. 221-228 ◽  
Author(s):  
Jason E. Coleman ◽  
Matthew J. Huentelman ◽  
Sergey Kasparov ◽  
Beverly L. Metcalfe ◽  
Julian F. R. Paton ◽  
...  
Keyword(s):  
Animal Studies ◽  
Large Scale ◽  
Lentiviral Vector ◽  
High Titer ◽  
Lentiviral Vectors ◽  
Efficient Production ◽  
Scale Production ◽  
Peripheral Tissues ◽  
Large Scale Production

The aim of this study was to develop an efficient method for packaging and concentrating lentiviral vectors that consistently yields high-titer virus on a scale suitable for in vivo applications. Transient cotransfection of 293T packaging cells with DNA plasmids encoding lentiviral vector components was optimized using SuperFect, an activated dendrimer-based transfection reagent. The use of SuperFect allowed reproducible and efficient production of high-titer lentiviral vector at concentrations greater than 1 × 107transducing units per ml (TU/ml) and required less than one-third of the total amount of DNA used in traditional calcium phosphate transfection methods. Viral titers were further increased using a novel concentration protocol that yielded an average final titer of 1.4 × 1010TU/ml. Lentiviruses produced using these methods exhibited efficient transduction of central nervous system and peripheral tissues in vivo. The method is reproducible and can be scaled up to facilitate the use of these vectors in animal studies.

Large-Scale Production of Pseudotyped Lentiviral Vectors Using Baculovirus GP64

2003 ◽  
Vol 14 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Mukesh Kumar ◽  
Brian P. Bradow ◽  
Joshua Zimmerberg
Keyword(s):  
Large Scale ◽  
Lentiviral Vectors ◽  
Scale Production ◽  
Large Scale Production

I. S. A. Baud. Forms of Production and Women's Labour: Gender Aspects of Industrialisation in India and Mexico. New Delhi: Sage Publications. 1992. 321 pp.Hardbound. Price: Indian Rs 295.00.

1993 ◽  
Vol 32 (1) ◽  
pp. 129-131
Author(s):  
Naureen Talha
Keyword(s):  
Large Scale ◽  
Indian Society ◽  
New Delhi ◽  
Scale Production ◽  
Female Labour ◽  
Self Employment ◽  
Commodity Production ◽  
Large Scale Production ◽  
Mexican Society ◽  
Small Production

The literature on female labour in Third World countries has become quite extensive. India, being comparatively more advanced industrially, and in view of its size and population, presents a pictures of multiplicity of problems which face the female labour market. However, the author has also included Mexico in this analytical study. It is interesting to see the characteristics of developing industrialisation in two different societies: the Indian society, which is conservative, and the Mexican society, which is progressive. In the first chapter of the book, the author explains that he is not concerned with the process of industrialisation and female labour employed at different levels of work, but that he is interested in forms of production and women's employment in large-scale production, petty commodity production, marginal small production, and self-employment in the informal sector. It is only by analysis of these forms that the picture of females having a lower status is understood in its social and political setting.

Sign in / Sign up

Export Citation Format

Share Document