scholarly journals Method to extract minimally damaged collagen fibrils from tendon

2016 ◽  
Vol 3 (4) ◽  
pp. e54
Author(s):  
Yehe Liu ◽  
Nelly Andarawis-Puri ◽  
Steven J. Eppell
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sea Cucumber ◽  
Dark Field ◽  
Collagen Fibrils ◽  
New Method ◽  
Tendon Tissue ◽  
Fibril Diameter ◽  
Rat Tail ◽  
Scanning Electron

 A new method is presented to extract collagen fibrils from mammalian tendon tissue. Mammalian tendons are treated with a trypsin-based extraction medium and gently separated with tweezers in an aqueous solution. Collagen fibrils released in the solution are imaged using both dark-field light microscopy and scanning electron microscopy. The method successfully extracts isolated fibrils from rat tail and patellar tendons. To examine whether the method is likely to damage fibrils during extraction, sea cucumber dermis fibril lengths are compared against those obtained using only distilled water. The two methods produce fibrils of similar lengths. This is contrasted with fibrils being shortened when extracted using a tissue homogenizer. Scanning electron microscopy shows the new method preserves D-banding features on fibril surfaces and that fibril diameter does not vary substantially compared with water extracted fibrils. 

scholarly journals C-S-H Pore Size Characterization Via a Combined Nuclear Magnetic Resonance (NMR)–Scanning Electron Microscopy (SEM) Surface Relaxivity Calibration

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1779 ◽  
Author(s):  
Christoph Naber ◽  
Florian Kleiner ◽  
Franz Becker ◽  
Long Nguyen-Tuan ◽  
Christiane Rößler ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Ion Beam ◽  
New Method ◽  
Sem Images ◽  
Surface Relaxivity ◽  
Scanning Electron ◽  
Nuclear Magnetic

A new method for the nuclear magnetic resonance (NMR) surface relaxivity calibration in hydrated cement samples is proposed. This method relies on a combined analysis of 28-d hydrated tricalcium silicate samples by scanning electron microscopy (SEM) image analysis and 1H-time-domain (TD)-NMR relaxometry. Pore surface and volume data for interhydrate pores are obtained from high resolution SEM images on surfaces obtained by argon broad ion beam sectioning. These data are combined with T2 relaxation times from 1H-TD-NMR to calculate the systems surface relaxivity according to the fast exchange model of relaxation. This new method is compared to an alternative method that employs sequential drying to calibrate the systems surface relaxivity.

A new method of wet scanning electron microscopy for the analysis of myelination in EAE mouse model of multiple sclerosis

2006 ◽  
Vol 57 (4) ◽  
pp. 291-297 ◽  
Author(s):  
Abraham Nyska ◽  
Miri Horowitz ◽  
Debbie Anaby ◽  
Alon Sabban ◽  
Ilit Leizerman ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Multiple Sclerosis ◽  
Scanning Electron Microscopy ◽  
Mouse Model ◽  
New Method ◽  
Scanning Electron

An Inexpensive Approach for Bright-Field and Dark-Field Imaging by Scanning Transmission Electron Microscopy in Scanning Electron Microscopy

2014 ◽  
Vol 20 (1) ◽  
pp. 124-132 ◽  
Author(s):  
Binay Patel ◽  
Masashi Watanabe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Scanning Transmission Electron Microscopy ◽  
Lattice Spacing ◽  
Dark Field ◽  
Bright Field ◽  
Specimen Holder ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Scanning Electron

AbstractScanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) is a convenient technique for soft materials characterization. Various specimen-holder geometries and detector arrangements have been used for bright-field (BF) STEM-in-SEM imaging. In this study, to further the characterization potential of STEM-IN-SEM, a new specimen holder has been developed to facilitate direct detection of BF signals and indirect detection of dark-field (DF) signals without the need for substantial instrument modification. DF imaging is conducted with the use of a gold (Au)-coated copper (Cu) plate attached to the specimen holder which directs highly scattered transmitted electrons to an off-axis yttrium-aluminum-garnet (YAG) detector. A hole in the copper plate allows for BF imaging with a transmission electron (TE) detector. The inclusion of an Au-coated Cu plate enhanced DF signal intensity. Experiments validating the acquisition of true DF signals revealed that atomic number (Z) contrast may be achieved for materials with large lattice spacing. However, materials with small lattice spacing still exhibit diffraction contrast effects in this approach. The calculated theoretical fine probe size is 1.8 nm. At 30 kV, in this indirect approach, DF spatial resolution is limited to 3.2 nm as confirmed experimentally.

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