scholarly journals Isolating astrocytes and neurons sequentially from postnatal murine brains with a magnetic cell separation technique

2014 ◽  
Vol 1 (2) ◽  
pp. 11 ◽  
Author(s):  
Maria Feldmann ◽  
Praneeti Pathipati ◽  
R Ann Sheldon ◽  
Xiangning Jiang ◽  
Donna M Ferriero
Keyword(s):  
Cell Separation ◽  
Separation Technique ◽  
Magnetic Cell Separation

Clinical Scale Enrichment and Expansion of Highly Pure CD25+Foxp3+ Regulatory T Cells.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 3718-3718
Author(s):  
Carina Conrads ◽  
Jürgen Schmitz ◽  
Mario Assenmacher ◽  
Claudia Niemand ◽  
Alexander Scheffold
Keyword(s):  
Clinical Application ◽  
Cell Separation ◽  
Foxp3 Expression ◽  
Clinical Scale ◽  
Organ Rejection ◽  
Immune Mediated ◽  
In Vitro Expansion ◽  
Magnetic Cell Separation ◽  
Effector Cytokines

Abstract Abstract 3718 CD25+Foxp3+ regulatory T cell (Treg) bear great potential to prevent or treat a variety of immune mediated diseases, including autoimmunity, organ rejection or GvHD. Currently Treg for clinical application can be separated by magnetic cell separation via the CliniMACS® Plus Instrument using CD25 enrichment plus/minus prior depletion of CD8 or CD19 positive cells. With this technology Treg can be enriched to a mean purity of about 50% and first clinical trials for prevention of GvHD show no adverse effects at all. Despite these promising results, concerns have been raised whether in the setting of organ transplantation or autoimmunity higher Treg purities and/or the in vitro expansion of Treg without loss of Foxp3+ expression are required. Therefore, we have optimized the parameters for CD25 enrichment via CliniMACS to achieve higher purity of the isolated Treg. The purity of Treg could be increased by about 20–30% resulting in an average purity of 70–80% of Foxp3+ Treg. We have also developed a protocol for the in vitro expansion of CliniMACS isolated Treg using CD3/CD28 coated MACSiBead™Particles. In the presence of Rapamycin CliniMACS isolated Treg could be expanded about 20 fold with a single round of stimulation. Importantly Foxp3+ expression was not affected by the expansion but remained constant at about 70–80%. Similarly the expression of effector cytokines by expanded Treg was greatly suppressed by Rapamycin. These data show that Treg for clinical application can efficiently be isolated with high purity via CliniMACS and subsequently be expanded in vitro without loss of Foxp3 expression. Disclosures: Conrads: Miltenyi Biotec: Employment. Schmitz:Miltenyi Biotec: Employment. Assenmacher:m: Employment. Niemand:Miltenyi Biotec: Employment. Scheffold:Miltenyi Biotec: Employment.

scholarly journals Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Daryoush Shahbazi-Gahrouei ◽  
Mohammad Abdolahi ◽  
Sayyed Hamid Zarkesh-Esfahani ◽  
Sophie Laurent ◽  
Corine Sermeus ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Cell Surface ◽  
Surface Antigen ◽  
Cell Separation ◽  
Cell Surface Antigen ◽  
Cell Surface Antigens ◽  
Vitro Assay ◽  
Gold Standard Method ◽  
Magnetic Cell Separation

Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis.

scholarly journals Utility of Magnetic Cell Separation as a Molecular Sperm Preparation Technique

2007 ◽  
Vol 29 (2) ◽  
pp. 134-142 ◽  
Author(s):  
T. M. Said ◽  
A. Agarwal ◽  
M. Zborowski ◽  
S. Grunewald ◽  
H.-J. Glander ◽  
...  
Keyword(s):  
Cell Separation ◽  
Preparation Technique ◽  
Sperm Preparation ◽  
Magnetic Cell Separation

scholarly journals The Origins and the Current Applications of Microfluidics-Based Magnetic Cell Separation Technologies

2022 ◽  
Vol 8 (1) ◽  
pp. 10
Author(s):  
Ozgun Civelekoglu ◽  
A. Bruno Frazier ◽  
A. Fatih Sarioglu
Keyword(s):  
Cell Separation ◽  
Cell Types ◽  
High Throughput Analysis ◽  
Precise Control ◽  
Micro Scale ◽  
Bulk Separation ◽  
Wide Range ◽  
Magnetic Cell Separation ◽  
Magnetic Gradients ◽  
Operational Simplicity

The magnetic separation of cells based on certain traits has a wide range of applications in microbiology, immunology, oncology, and hematology. Compared to bulk separation, performing magnetophoresis at micro scale presents advantages such as precise control of the environment, larger magnetic gradients in miniaturized dimensions, operational simplicity, system portability, high-throughput analysis, and lower costs. Since the first integration of magnetophoresis and microfluidics, many different approaches have been proposed to magnetically separate cells from suspensions at the micro scale. This review paper aims to provide an overview of the origins of microfluidic devices for magnetic cell separation and the recent technologies and applications grouped by the targeted cell types. For each application, exemplary experimental methods and results are discussed.

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