scholarly journals Histological Study of Respiratory Organ of Betta sp.

2019 ◽  
Vol 2 ◽  
pp. 181-184
Author(s):  
Nurul Safitri Apriliani ◽  
Hikmah Supriyati ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Enzyme Production ◽  
Histological Study ◽  
Ornamental Fish ◽  
Respiratory Organ ◽  
Gas Bladder ◽  
Low Volume ◽  
Gill Lamellae ◽  
Gill Raker ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Betta sp. is a freshwater ornamental fish which also known as a fighting fish. One of the fundamental organs to support fishes life is respiratory organ. Fighting fish is belongs to the suborder Anabantoidei which means labyrinth fishes group. The aim of the study was to know histology of the respiratory organs of Betta sp. Histological preparations were done using paraffin method, stained with Hematoxylin-Eosin (HE). The result showed that Betta sp. has a respiratory organ common fish i.e gills and additional respiratory organ structure namely labyrinth and pseudobranch that makes Betta sp can survive in a low volume of water. The gill is consists of gill arch, gill raker, gill fillament and gill lamellae. The labyrinth is consist of connective tissue and folded ephitelium. Pseudobranch according to some literature function as an additional respiratory. Functions attributed to the pseudobranch include; regulation of oxygen to the eyes, enzyme production for use in the gas bladder, osmoregulation, and many others.

scholarly journals Histological Study of Mudskipper (Periophthalmus gracilis) Gills

2019 ◽  
Vol 2 ◽  
pp. 177-179
Author(s):  
Hikmah Supriyati ◽  
Nurul Safitri Apriliani ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Histological Study ◽  
Gill Filament ◽  
Histological Structure ◽  
Respiratory Organ ◽  
Secondary Lamellae ◽  
Long Time ◽  
Qualitative Descriptive ◽  
Gill Structure ◽  
And Behavior ◽  
Hematoxylin Eosin

Mudskipper belongs to the Gobiidae family which has respiratory adaptation to fits their habitat. Mudskipper has a different gill structure or modification of gills, this different structure allows the mudskipper to survive for a long time outside water. This study aimed to determine the histology of gills and find out whether there is a modification of gills in the mudskipper respiratory organs (Periophthalmus gracilis). Histological preparations were done using paraffin method, stained with Hematoxylin-Eosin. Data analysis was carried out in a qualitative descriptive. The results showed that there is no additional respiratory organ in the mudskipper respiration, whereas the gills have some modifications. The histological structure of mudskipper gills consists of gill arches, arteries, gill filament, primary lamellae, and secondary lamellae. The gills of mudskipper have a different structure from the general fish, which has a thick secondary lamellae with a low amount of density, the shape of the filament are short and bent. This gill structure is a form of adaptation to habitat and behavior to live outside the water in a relatively long time.

Studi Histokimia Usus Biawak Air (Varanus salvator bivittatus) (Reptil: Varanidae)

2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ernawati
Keyword(s):  
Tissue Sample ◽  
Histological Section ◽  
Tissue Perfusion ◽  
Intestinal Wall ◽  
Biological Information ◽  
Suitable Habitat ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Biological information, behaviour and suitable habitat of water monitor was very less in order to support its maintenance management and breeding efforts. One of important information is the information of digestive tract, particularly the information about the structure of intestine tissue of water monitor. Sample in this research was intestine organ of water monitor. The animal was anesthetized, exanguinated, and fixed in paraformaldehyde 4% by tissue perfusion method. The intestine tissue sample for histological section with paraffin method was cutted with 3-4 μm thick and coloured with hematoxylin eosin (HE). Observation were performed to the structure of intestine histology. The results was analysed descriptively and presented in figures. Monitor lizard intestine consist of small intestine and large intestine. The small intestinal wall was observed similar to jejunum and ileum. The large intestinal wall was composed of transitional ephytelia and connective tissue. However, the ephytelial layer in this tissue was composed of transitional ephytelia that similar to vesica urinaria and there are no villi.

scholarly journals Tracheotomy in growing rats: histological aspects

2003 ◽  
Vol 18 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Mônica Cecília Bochetti Manna ◽  
Edna Frasson de Souza Montero ◽  
Maria Antonieta Longo Galvão da Silva ◽  
Yara Juliano
Keyword(s):  
Inflammatory Infiltrate ◽  
Sham Group ◽  
Histological Study ◽  
Reference Pattern ◽  
Microsurgical Technique ◽  
Growing Rats ◽  
Global Comparison ◽  
Different Types ◽  
Hematoxylin Eosin ◽  
Epidermoid Metaplasia

PURPOSE: To compare morphologically three different types of tracheotomy in growing rats, applying microsurgical technique. METHODS: EPM-1 Wistar growing rats (n=57) weighing 88gm and aged 35 days were randomized in four groups, according tracheotomy incision type (longitudinal, transverse and tracheal segment excision), and sham group. Following intramuscular anesthesia with ketamine and xylazine, the trachea was exposed and incised, according to the group, and a hand-made endotracheal cannula was inserted into the organ, under sterile conditions. This cannula was removed after 7 days, and animals have been sacrificed 30 days later. Tracheas samples were submitted to histological study, stained by hematoxylin-eosin and Masson trichrome, evaluating fibrosis, inflammatory infiltrate and epidermoid metaplasia. RESULTS: There was more frequency of inflammatory infiltrate at the tracheal epithelium in the tracheal segment excision group (87%) compared to the longitudinal (40%) and transverse (36%) incision groups (p=0.009). Evaluating epidermoid metaplasia, tracheal segment excision and the longitudinal groups presented 33% and 40%, respectively, compared to 0% of the transverse group (p=0.03). Concerning to fibrosis, in a global comparison (p=0.1) among the three groups there was no difference, however, compared to the longitudinal group the transverse group showed lower level of fibrosis (p=0.04). Sham group did not present any relevant morphologic alterations and it was used as reference pattern. CONCLUSION: Taken together, our data show that tracheal segment excision promotes more epithelium aggression and transverse tracheal incision shows less morphologic alterations.

scholarly journals Histological analysis of the tongue, esophagus and crop of Southern Carcará (Polyborus plancus)

2021 ◽  
Vol 10 (2) ◽  
pp. e21410212439
Author(s):  
Vanessa Sobue Franzo ◽  
Leiny Paula de Oliveira ◽  
Ana Carolina Pôssas Viana ◽  
Adriano Tony Ramos ◽  
Jéssica Fernanda Bertolino ◽  
...  
Keyword(s):  
Digestive System ◽  
Histological Analysis ◽  
Squamous Epithelium ◽  
Eating Habits ◽  
Histological Study ◽  
Pekin Ducks ◽  
The Family ◽  
Body Weights ◽  
Stratified Squamous Epithelium ◽  
Hematoxylin Eosin

The Southern Caracara is a bird of prey, of the family Falconidae, inhabits all the Brazilian territory, they are omnivorous birds adapted for hunting. The digestive system of the predators presents differences in their morphology due to their eating habits. Knowing the importance of the gastrointestinal system, this study aims to carry out a histological study of the tongue, esophagus and the crop of Polyborus plancus. For this purpose, eight avian of both sexes, with different body weights and ages were used, they were euthanized and the organs were collected, fixed, dehydrated and stained with hematoxylin-eosin. It was noted that the tongue has stratified squamous epithelium with mucous glands and with different degrees of keratinization. The esophagus and crop have four well developed muscle layers and mucous glands. Additionally, in the crop there is presence of an adventitious layer that consists of loose connective tissue. We conclude that the tongue, the esophagus and the crop of the Southern Caracara have similar histological organization of several birds like the Pekin Ducks, ostriches, domestic chicken and partridges.

scholarly journals Lung Histopathology of Laying Hens Infected by Colibacillosis in the Animal Cages Experiments of the Disease Investigation Center 6, Denpasar, Bali

2019 ◽  
Vol 3 (2) ◽  
pp. 25
Author(s):  
Kadek Ayu Trisna Yanti ◽  
Iriani Setyawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Laying Hens ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Gallus Gallus Domesticus ◽  
Directorate General ◽  
Lung Histopathology ◽  
Live Stock ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Neutral Buffer Formalin

This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.

scholarly journals Study of Shark Kidney Histology (Carcharhinus sorrah)

2017 ◽  
Vol 1 ◽  
pp. 67-69
Author(s):  
Zakia Darajat
Keyword(s):  
Microscopic Observation ◽  
Lymphoid Tissue ◽  
Animal Species ◽  
Cartilaginous Fish ◽  
Proximal Tubules ◽  
Bony Fishes ◽  
Distal Tubules ◽  
Different Characteristics ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Shark is a fish widespread in the tropical Indo-Pacific Ocean with a depth of 75 to 130 meters. Shark is a cartilaginous fish (Elasmobranchii). The fish is an ancient animal species that are still alive and also have different characteristics with bony fishes. Research on the histology of the shark's kidneys is still rare. The purpose of this study was to describe the histology of the shark kidneys (Carcharhinus sorrah). In this study we used one individual shark (Carcharhinus sorrah) from Depok Beach, Yogyakarta. The method used in this research was paraffin method with Hematoxylin-Eosin staining. From microscopic observation, the kidneys consist of glomerular parts, proximal tubules, distal tubules and lymphoid tissue.

scholarly journals STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator))

2016 ◽  
Vol 10 (2) ◽  
pp. 153-156 ◽  
Author(s):  
Hamny Hamny ◽  
Muhammad Iqbal ◽  
Sri Wahyuni ◽  
Mustafa Sabri ◽  
M. Jalaluddin ◽  
...  
Keyword(s):  
Histological Study ◽  
Histological Structure ◽  
Ventral Lobe ◽  
Nacl Solution ◽  
Dorsal Lobe ◽  
Yellow Color ◽  
Monitor Lizard ◽  
Oval Form ◽  
Varanus Salvator ◽  
Hematoxylin Eosin

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.45±0.64 cm (dorsal lobe) and 7.00±0.42 cm (ventral lobe). Weight of dorsal lobe was 0.15±0.01 g and ventral lobe was 6.35±2.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.

scholarly journals Comparative Anatomy of Labyrinth and Gill of Catfish (Clarias gariepinus) (Burchell, 1822) and Snakehead Fish (Channa striata) (Bloch, 1793)

2018 ◽  
Vol 7 (2) ◽  
pp. 39-43
Author(s):  
Ina Karlina ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Blood Vessels ◽  
Fish Species ◽  
Respiratory System ◽  
Comparative Anatomy ◽  
Clarias Gariepinus ◽  
Low Oxygen ◽  
Gill Structure ◽  
Channa Striata ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Dumbo catfish (Clarias gariepinus) and Gabus (Channa striata) have additional organ respiratory system structures in the gills, called labyrinth. This organ is a tool for fish to take oxygen directly from the air so that it can live in low oxygen habitats. Both fish have differences at the Order level. Catfish is an order of Siluriformes as Gabus is an order of Perciformes. The purpose of this study was to distinguish the anatomy and histology of the gill structure and labyrinth in both fish. The macroanatomy observation was conveyed by dissection to determine the gill topography in the two fish species. Histology preparations were carried out using the paraffin method and using Hematoxylin-Eosin (HE) staining that showed in the labyrinth organ there were many blood vessels to bind oxygen then distributed to all parts of the fish's body.

scholarly journals Effect of vydate gill histology in female mosquito fish Gambusia affinis (Baird and Girard)

2017 ◽  
Vol 14 (1) ◽  
pp. 1-10
Author(s):  
Baghdad Science Journal
Keyword(s):  
Histological Study ◽  
Gambusia Affinis ◽  
Chloride Cells ◽  
Control Group ◽  
University Campus ◽  
Histological Changes ◽  
Histological Alterations ◽  
Mosquito Fish ◽  
Secondary Lamellae ◽  
Gill Lamellae

This study aimed to identify the histological changes of mosquito fish (Gambusia affinis) exposed to vydate pesticide. (48) female fishes were collected from local markets and also from the stream that surround Baghdad university campus at Al-jadyria, fishes divided into (4) groups and each group consist of 12 fish: T1(exposed to 0.0000001 mg/L),T2 (exposed to 0.0000002 mg/L ) and T3 (exposed to 0.000001 mg /L) of vydate pesticide for (8) weeks. As well as a control group. Fishes were dissected for histological preparations after each (2,4,6,8) weeks of exposure time. Gills were fixed by Bouin’s fluid and stained with Hematoxylin and eosin. Histological study showed that the structure of gill in a control group consists of primary and secondary gill lamellae appeared parallel and oblique. The secondary gill lamellae lined with squamous epithelial and pillar cells, and each secondary lamellae have capillary derived from blood vessel in primary lamellae, chlorid cells and few mucous cells found between secondary lamellae. The histological alterations in gills included hyperplasia of chloride cells, congestion of blood vessels, lamellar disarray, lifting of epithelial cells, Oedema, fusion of adjacent secondary lamella and Aneurysm. Results concluded that vydate had a vicious effect on gills and caused severe histological alterations such as hyperplasia of chloride cells and simple alterations such as lamellar disarray, and also these histological changes commensurate direct proportion with concentrations were used in this study.

scholarly journals Anatomical and Histological Study of Shark (Carcharhinus sorrah) Kidney

2019 ◽  
Vol 8 (2) ◽  
pp. 37-40
Author(s):  
Hikmah Supriyati ◽  
Rakhmiyati Rakhmiyati ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Sea Water ◽  
Histological Study ◽  
Head Kidney ◽  
Cartilaginous Fish ◽  
The Body ◽  
Histological Structure ◽  
Descriptive Data ◽  
Water Fish ◽  
Qualitative Descriptive ◽  
Paraffin Method

Sharks are sea water fishes belong to the class Chondrichthyes, Subclass Elasmobranchii. Sharks are cartilaginous fish that have a different osmoregulation process than any other sea water fish. Cartilaginous fish is the only vertebrate that can maintain urea. This study aims to determine the anatomical and histological structure of the kidney in the anterior, medial and posterior parts of kidney. The study was conducted by observing anatomy of the kidney. Histological preparations were made using the paraffin method. Qualitative descriptive data analysis was done. Research results show that shark kidneys consist of three parts, namely the head kidney, the body kidney, and the tail kidney. Kidney sharks are brownish red with a size of 18 cm long. Histological observations of shark kidney in the head kidney reveals many glomerulus, body kidney reveals many distal and tubule proximal contractile tubules whereas tail kidney reveals stroma that is rarely found in vertebrate kidney.

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