scholarly journals Anatomical Structure of Shark Fish Digestive (Carcharhinus sorrah)

2019 ◽  
Vol 2 ◽  
pp. 169-171
Author(s):  
Ulfaricha Cahya Happyalita ◽  
Nur Lailatul Kamila
Keyword(s):  
Microscopic Observation ◽  
Anatomical Structure ◽  
Digestive Organs ◽  
Macroscopic Observation ◽  
Hematoxylin Eosin ◽  
Paraffin Method

This study aims to determine the anatomical structure of sharks. Sharks are members of the subclass elasmobranchii. Sharks (Carcharhinus sorrah) are shaped like terpedoit. This research was conducted using macroscopic and microscopic observations on the digestive organs of sharks (Carcharhinus sorrah). Macroscopic observation is done by looking at shapes, structures, and analyzing photos of organs. Microscopic observation was carried out by making organ preparations using paraffin method, using hematoxylin-eosin staining then observed using a microscope. Digestive organs of sharks (Carcharhinus sorrah) were observed, namely, stomach, intestine and liver.

scholarly journals Study of Shark Kidney Histology (Carcharhinus sorrah)

2017 ◽  
Vol 1 ◽  
pp. 67-69
Author(s):  
Zakia Darajat
Keyword(s):  
Microscopic Observation ◽  
Lymphoid Tissue ◽  
Animal Species ◽  
Cartilaginous Fish ◽  
Proximal Tubules ◽  
Bony Fishes ◽  
Distal Tubules ◽  
Different Characteristics ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Shark is a fish widespread in the tropical Indo-Pacific Ocean with a depth of 75 to 130 meters. Shark is a cartilaginous fish (Elasmobranchii). The fish is an ancient animal species that are still alive and also have different characteristics with bony fishes. Research on the histology of the shark's kidneys is still rare. The purpose of this study was to describe the histology of the shark kidneys (Carcharhinus sorrah). In this study we used one individual shark (Carcharhinus sorrah) from Depok Beach, Yogyakarta. The method used in this research was paraffin method with Hematoxylin-Eosin staining. From microscopic observation, the kidneys consist of glomerular parts, proximal tubules, distal tubules and lymphoid tissue.

Studi Histokimia Usus Biawak Air (Varanus salvator bivittatus) (Reptil: Varanidae)

2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ernawati
Keyword(s):  
Tissue Sample ◽  
Histological Section ◽  
Tissue Perfusion ◽  
Intestinal Wall ◽  
Biological Information ◽  
Suitable Habitat ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Biological information, behaviour and suitable habitat of water monitor was very less in order to support its maintenance management and breeding efforts. One of important information is the information of digestive tract, particularly the information about the structure of intestine tissue of water monitor. Sample in this research was intestine organ of water monitor. The animal was anesthetized, exanguinated, and fixed in paraformaldehyde 4% by tissue perfusion method. The intestine tissue sample for histological section with paraffin method was cutted with 3-4 μm thick and coloured with hematoxylin eosin (HE). Observation were performed to the structure of intestine histology. The results was analysed descriptively and presented in figures. Monitor lizard intestine consist of small intestine and large intestine. The small intestinal wall was observed similar to jejunum and ileum. The large intestinal wall was composed of transitional ephytelia and connective tissue. However, the ephytelial layer in this tissue was composed of transitional ephytelia that similar to vesica urinaria and there are no villi.

scholarly journals Lung Histopathology of Laying Hens Infected by Colibacillosis in the Animal Cages Experiments of the Disease Investigation Center 6, Denpasar, Bali

2019 ◽  
Vol 3 (2) ◽  
pp. 25
Author(s):  
Kadek Ayu Trisna Yanti ◽  
Iriani Setyawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Laying Hens ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Gallus Gallus Domesticus ◽  
Directorate General ◽  
Lung Histopathology ◽  
Live Stock ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Neutral Buffer Formalin

This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.

scholarly journals STRUKTUR ANATOMIS VALVULA INTESTINALIS PARI GITAR (Rhinobatos typus)

2019 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Kusrini Kusrini ◽  
La Aba
Keyword(s):  
Anatomical Structure ◽  
Internal Part ◽  
Habitat Differences ◽  
Food Absorption ◽  
Paraffin Method

<p><em>Rhinobatos typus is included in Elasmobranchii Subclasses which has a unique intestine structure on internal part forms folds called valvula intestinalis as extension surface of food absorption. The uniqueness is influenced by habitat differences and food types. Ray’s habitat is in all waters, one of them is in waters Wakatobi Island. The aims of this research to determine Rhinobatos typus anatomical structure variations of valvula intestinalis were decided by observing intestine internal folds variations. Microanatomy structures were performed by making histological preparations using paraffin method, NBF (netral buffer formalin) solution fixation with Hematoxilin-Eosin (HE) staining. The result of the research indicated microanatomis structures of Rhinobatos typus has valvula intestinalis in spiral-shaped. Microanatomic structures of both internals valvula intestinalis from outside to inside consists of mucosa, submucosa, and muscularis.</em><em></em></p>

scholarly journals Microscopic Characterization of Fusarium sp. Associated with Yellow Disease of Pepper (Piper nigrum L.) in South Bangka Regency

2021 ◽  
Vol 9 (1) ◽  
pp. 1-9
Author(s):  
Ayu Lestari ◽  
Henri Henri ◽  
Eka Sari ◽  
Tri Wahyuni
Keyword(s):  
Microscopic Observation ◽  
Sampling Technique ◽  
Piper Nigrum ◽  
Pepper Plant ◽  
Colony Diameter ◽  
Colony Color ◽  
Macroscopic Observation ◽  
Fusarium Sp ◽  
Orange Color

Pepper production has decreased recently, especially due to yellow diseases of Fusarium sp. Thus, this research aimed to isolate and characterize Fusarium sp. from soil and root of healthy and diseased pepper plants. The sampling technique used was purposive sampling. Soil and root pepper samples were taken from lands in Payung and Ranggung Village, Payung District, South Bangka Regency. There were 3 varieties of pepper plant used, including Petaling 1, Nyelungkup, and Merapin Daun Kecil. The characterization of Fusarium sp. isolate included macroscopic and microscopic observation. Macroscopic observation included colony color, colony base color, and growth rate/colony diameter size (cm), while microscopic observation included hyphae structure, and the shape and size of microconidia, macroconidia, chlamydospore, and conidiophore. The research found 66 isolates of Fusarium genus based on the colony color. Most of the isolates were white or purple and red. Colony color of Fusarium sp. showed white color, which then turned to orange color. All isolates showed septate hyphae. Isolates with macroconidia 3-4 septate and micronidia 0-1 septate showed the character of Fusarium oxysporum, while isolates with macroconidia 3-5 septate and microconidia 0-2 septate showed the character of Fusarium solani.

scholarly journals JUMLAH TRAKEA PADA DAUN LUDWIGIA ADSCENDENS (L.) HARA DALAM KONDISI TERGENANG : SEBUAH GEJALA PLASTISITAS ANATOMI ##plugins.themes.bootstrap3.article.sidebar##

1999 ◽  
Vol 4 (2) ◽  
pp. 111
Author(s):  
Joko Budiono
Keyword(s):  
Cross Section ◽  
Cross Sections ◽  
Water Depth ◽  
Vascular Bundle ◽  
Water Surface ◽  
Anatomical Structure ◽  
Leaf Primordium ◽  
Aquatic Weed ◽  
Paraffin Method ◽  
Ludwigia Adscendens

This study was conducted to reveal the influence of waterlogging on the number of tracheas in each vascular bundle of Ludwigia adscendens (L.) Hara leaf. This plant is well known as aquatic weed which can grow floating on water surface. The leaf primordium extend across the water from 10-15 cm below water surface. The plant was grown in aquarium at 0, 10 and 15 cm water depth. The observed leaves were first, third and fifth the base. Anatomical structure were analyzed using serial cross sections of he leaf the cross section were made by paraffin method. The data were analyzed using ANOVA and DMRT method. The result showed that significance difference on the number of tracheas in each vascular bundle were found among leaves of different depth.

scholarly journals Comparative Anatomy of Labyrinth and Gill of Catfish (Clarias gariepinus) (Burchell, 1822) and Snakehead Fish (Channa striata) (Bloch, 1793)

2018 ◽  
Vol 7 (2) ◽  
pp. 39-43
Author(s):  
Ina Karlina ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Blood Vessels ◽  
Fish Species ◽  
Respiratory System ◽  
Comparative Anatomy ◽  
Clarias Gariepinus ◽  
Low Oxygen ◽  
Gill Structure ◽  
Channa Striata ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Dumbo catfish (Clarias gariepinus) and Gabus (Channa striata) have additional organ respiratory system structures in the gills, called labyrinth. This organ is a tool for fish to take oxygen directly from the air so that it can live in low oxygen habitats. Both fish have differences at the Order level. Catfish is an order of Siluriformes as Gabus is an order of Perciformes. The purpose of this study was to distinguish the anatomy and histology of the gill structure and labyrinth in both fish. The macroanatomy observation was conveyed by dissection to determine the gill topography in the two fish species. Histology preparations were carried out using the paraffin method and using Hematoxylin-Eosin (HE) staining that showed in the labyrinth organ there were many blood vessels to bind oxygen then distributed to all parts of the fish's body.

scholarly journals Uji Toksisitas Surfaktan Linear Alkilbenzene Sulfonate (LAS) Histologi Insang dan Hati Benih Ikan Kerapu Bebek (Cromileptes altivelis)

2019 ◽  
Vol 8 (1) ◽  
pp. 107-116
Author(s):  
Devi Kristianti ◽  
Resty Paramitha ◽  
Fitri Agustriani ◽  
Gusti Diansyah
Keyword(s):  
Side Effects ◽  
Experimental Method ◽  
Liver Damage ◽  
Tissue Damage ◽  
Surfactant Concentration ◽  
Liver Histology ◽  
Toxic Effects ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Kristianti et al, 2019. Toxicity of Linear Alkilbenzene Sulfonate (LAS) Surfactant Gills and Liver Histology of Humpback Grouper Seed (Cromileptes Altivelis). JLSO 8(1):107-116.LAS is surfactant that is most commonly used because this surfactant is the main cleaning substance contained in detergent. Waste of detergent can give side effects in the tissue of organisms, especially on the gills and liver of fish. The aims of this research were to analyze the toxic effects arised from LAS surfactant to the survival of fish seed experiment with different concentrations, to determine levels of LAS surfactant that are capable to be tolerated and to analyze the gills and liver damage arising from LAS surfactant to fish seed experiment. The test of LAS surfactant toxicity on fish seed experiment was conducted using the laboratory of experimental method, while the testing of gills and liver histology of fish seed experiment with paraffin method and Hematoxylin Eosin (HE) staining. The results showed that LAS surfactant gave toxic effects on Humpback Grouper Seed (Cromileptes altivelis) and gave effect such damage to the gills and liver histology. Toxicity and tissue damage rose as the increase of LAS surfactant concentration. LAS surfactant levels were able to be tolerated by Humpback Grouper Seed test not more than 0,253 mg/l.

scholarly journals Anatomical and Histological Structure of Black Pomfret Fish Kidney (Formio niger)

2017 ◽  
Vol 1 ◽  
pp. 71-74
Author(s):  
Nurul Safitri Apriliani
Keyword(s):  
Growth Rate ◽  
Fish Species ◽  
Economic Value ◽  
Histological Structure ◽  
Lymphoid Tissues ◽  
The Many ◽  
Fish Kidney ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Fast Growth Rate

Black pomfret is one of the many fish species found in the southern sea. Pomfret has high economic value and is an active swimmer that has fast growth rate, resistant to disease, and easy to maintain. The purpose of this research was to know the structure of anatomy and histology of black pomfret’s kidney. Histological slides were made using paraffin method and stain with Hematoxylin-Eosin. The results showed that kidney of black pomfret have a soft texture, and the colour is red degradation brown. Histologically, the kidney is found a glomerolous, tubules and lymphoid tissues.

scholarly journals Comparative Anatomy and Histology of Digestive Organs of Fruit-Eating Bats (Pteropus Vampyrus Linnaeus, 1758) and Insect-Eating Bats (Rhinolopus pusillus Temminck, 1834)

2019 ◽  
Vol 2 ◽  
pp. 37-40
Author(s):  
Desi Arsita Yani ◽  
Wanda Nurma Yuliyantika
Keyword(s):  
Comparative Anatomy ◽  
Digestive Organ ◽  
Insectivorous Bats ◽  
Digestive Organs ◽  
The Difference ◽  
Pteropus Vampyrus ◽  
And Behavior ◽  
Hematoxylin Eosin

Animal Bat consists of Megachiroptera and Microchiroptera. The difference between the two bats lies in the type and behavior of eating. Megachiroptera is a fruit-eating bat, while Microchiroptera is an insect-eating bat. This study aims to compare the digestive organ of fruit-eating bats (Pteropus vampyrus) and insect-eating bats (Rhinolopus pusillus). This research was conducted by observing macroanatomy and microanatomy in the digestive organ of both bats. Macroanatomy observation is done by looking at the shape and curvature of the analysis through organ photo media. Microanatomy observation was done by making organ preparations with hematoxylin-eosin staining and observed using a microscope. The digestive organ of fruit-eating bats and insectivores consists of the esophagus, stomach, duodenum and rectum. Macroanatomically, fruit-eating bats and insectivores differ in size, where the fruit-eating bat's digestive organs are larger than insectivorous bats. Microanatomically, fruit-eating bats and insectivores have relatively similar histological structures, which differ only in the size of the cell.

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