scholarly journals A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species

2016 ◽  
Vol 113 ◽  
pp. 265-269 ◽  
Author(s):  
Madougou GARBA ◽  
Anne LOISEAU ◽  
Laure BENOIT ◽  
Nathalie GAUTHIER
Keyword(s):  
Population Genetics ◽  
Microsatellite Loci ◽  
Parasitic Wasp ◽  
Molecular Tools ◽  
Habrobracon Hebetor ◽  
Polymorphic Microsatellite

scholarly journals Microsatellite loci development and population genetics in Neotropical fish Curimata mivartii (Characiformes: Curimatidae)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5959 ◽  
Author(s):  
Ricardo M. Landínez-García ◽  
Edna J. Marquez
Keyword(s):  
Population Genetics ◽  
Microsatellite Loci ◽  
Molecular Tools ◽  
Migratory Species ◽  
Lower Section ◽  
Neotropical Fish ◽  
International Union ◽  
Commercially Important ◽  
Species Specific ◽  
Near Threatened

The Curimatidae family plays an ecological role in the recycling and distribution of nutrients and constitutes a major food source for several commercially important fishes. Curimata mivartii, a member of this family, is considered a short-distance migratory species (≤100 km), categorized by the International Union for Conservation of Nature as a near threatened species, based on its declining population densities and habitat disturbance and fragmentation. Since population genetics and species-specific molecular tools remain unknown for all members of the Curimatidae family, this study developed a set of microsatellite loci and studied the population genetics of C. mivartii in the lower section of the Colombian Magdalena-Cauca basin. The results showed high levels of genetic diversity and evidence of gene flow even between locations separated over 350 km. This information provides a baseline for designing conservation and management programs for C.mivartii and constitutes the first study of population genetics in Curimatidae.

scholarly journals Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci

2020 ◽  
Vol 68 (3) ◽  
Author(s):  
Ricardo M. Landínez-García ◽  
Edna J. Márquez
Keyword(s):  
Population Genetics ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
International Union ◽  
Genetic Population ◽  
Genetic Diversity And Structure ◽  
Polymorphic Microsatellite ◽  
Species Specific ◽  
Low Coverage ◽  
Risk Categorization

Introduction: The freshwater fish Brycon henni (Characiformes: Bryconidae) is endemic to Colombia and currently considered as “least concern” according to the International Union for Conservation of Nature (IUCN). Objective: To develop microsatellite markers to examine population genetics in Brycon henni. Methods: Using a low-coverage sequenced genomic library, this study developed the first set of microsatellite loci to study the population genetics of this Neotropical species. These loci were used to evaluate the genetic diversity and structure of B. henni from three sites of the Magdalena-Cauca Basin (Colombia). Results: A set of twenty-one polymorphic microsatellite loci was highly informative and revealed that B. henni is evenly genetically structured between two tributaries of the Cauca River separated by only 30 km, a finding that indicates it conforms to reproductively isolated groups. Conclusions: We reported a set of twenty-one polymorphic microsatellite loci that was highly informative and allowed the detection of genetic structure at local and regional scales. This genetic population structure, concordant with that found in eight congeners, is relevant to estimate the B. henni risk categorization as well as for management, conservation, and restocking programs.

scholarly journals Development of thirty-four new microsatellite loci and multiplexing of seven existing loci for Zootoca vivipara (Squamata: Lacertidae)

2017 ◽  
Vol 16 (1) ◽  
pp. 89 ◽  
Author(s):  
Jose Luis Horreo ◽  
Maria Luisa Peláez ◽  
Teresa Suárez ◽  
Benoit Heulin ◽  
Patrick Stefan Fitze
Keyword(s):  
Population Genetics ◽  
Population Dynamics ◽  
Sexual Selection ◽  
Microsatellite Loci ◽  
Population Studies ◽  
Effective Number ◽  
Model Species ◽  
Common Lizard ◽  
Zootoca Vivipara ◽  
Polymorphic Microsatellite

Few microsatellite loci exist for the European common lizard, Zootoca vivipara, a common model species in studies of population dynamics, sexual selection, population genetics, parity evolution, and physiology. The existing primers did not amplify in all lineages, and multiplexes were not optimized. A total of 34 new polymorphic microsatellite markers have been developed for this species and tested in 64 specimens belonging to oviparous and viviparous clades (B and D). The microsatellites were combined into seven different multiplexes. Results showed that all but one loci successfully amplified in all samples and both clades. The number of alleles detected per locus ranged 7–22 alleles and the effective number 1.58–7.82. The observed heterozygosity ranged 0.312–0.930, showing that all loci were highly variable. Oviparous and viviparous clades exhibited significant genetic differences (in FST). In addition to these new markers, the seven previously published and widely used microsatellite loci have been multiplexed and tested in oviparous clades. These innovations will allow for timesaving and robust analyses in Zootoca vivipara, boosting evolutionary and population studies and easing paternity analyses

Genetic Diversity and Structure of the Nile Soft-Shelled Turtle (Trionyx triunguis) Along the Mediterranean coast of Turkey

2020 ◽  
Vol 27 (6) ◽  
pp. 307-315
Author(s):  
Özgür Güçlü ◽  
Bülent Bozdoğan
Keyword(s):  
Microsatellite Loci ◽  
Geographic Distance ◽  
Iucn Red List ◽  
Genetic Variations ◽  
Mediterranean Coast ◽  
Critically Endangered ◽  
Mediterranean Populations ◽  
Genetic Diversity And Structure ◽  
Polymorphic Microsatellite ◽  
The Mediterranean

The Nile soft-shelled turtle (Trionyx triunguis) is distributed between Dalyan and Samandağ throughout the Mediterranean coast in Turkey. The Mediterranean subpopulation of the Nile soft-shelled turtle is listed as critically endangered in the IUCN Red List Categories. This investigation aimed to determinate levels of genetic variations and patterns of genetic structures among Mediterranean populations in Turkey by using T. triunguis-specific microsatellite primers. A total of 13 polymorphic microsatellite loci were studied among samples of 121 individuals collected from five populations in Turkey. Of 13 polymorphic microsatellite loci used, 3 new were identified in this study. The genetic differentiation among the 5 studied populations of T. triunguis was significant (p 0.001). The analysis of molecular variance (AMOVA) indicated that genetic variations occurred mainly within populations (89.7%) rather than among populations (10.3%). Structure analysis showed presence of two main groups among the Mediterranean T. triunguis populations. However genetic variations among populations were not correlated with geographic distance between the locations. Analysis of data showed that one of the populations (Dalyan) had undergone a bottleneck effect. Migration analysis indicates that T. triunguis migrates between five Mediterranean populations in Turkey. We concluded that based on our results the status of ‘critically endangered’ of T. triunguis should be maintained. Long term population genetic survey studies should be undertaken and changes in habitats of T. triunguis populations, as well as their population size and structure should be monitored for each population to be able to establish a clear strategy for protection of T. triunguis.

scholarly journals Stand out from the Crowd: Small-Scale Genetic Structuring in the Endemic Sicilian Pond Turtle

Diversity ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 343
Author(s):  
Luca Vecchioni ◽  
Federico Marrone ◽  
Marco Arculeo ◽  
Uwe Fritz ◽  
Melita Vamberger
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Small Scale ◽  
Moderate Degree ◽  
Geographical Pattern ◽  
Genetic Structuring ◽  
Pond Turtle ◽  
Emys Trinacris ◽  
Polymorphic Microsatellite ◽  
Entire Distribution

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation of the Sicilian wetlands played a major role in this process. Finally, some conservation and management suggestions aimed at preventing the loss of genetic variability of the species are briefly reported, stressing the importance of considering the five detected clusters as independent Management Units.

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