Experimental Study of Chloride Diffusion Properties of Mortar Mixed with Ion-Exchange Resin

2018 ◽  
Vol 115 (5) ◽  
Author(s):  
Yao Luan ◽  
Osamu Sanada ◽  
Hiroshi Mutsuyoshi
Keyword(s):  
Experimental Study ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Chloride Diffusion ◽  
Diffusion Properties

Experimental Study on Chloride Diffusion, Strength and Fresh Properties of Ion-Exchange Resin Mixed Grout

2016 ◽  
Vol 711 ◽  
pp. 436-443
Author(s):  
Osamu Sanada ◽  
Hiroshi Mutsuyoshi ◽  
Luan Yao ◽  
Atsushi Sumida
Keyword(s):  
Ion Exchange ◽  
Prestressed Concrete ◽  
Exchange Resin ◽  
Chloride Ion ◽  
Ion Exchange Resin ◽  
Chloride Ions ◽  
Concrete Bridges ◽  
Chloride Diffusion ◽  
Chloride Attack ◽  
Increased Strength

In Japan, a number of existing prestressed concrete bridges are suffering chloride attack. Especially in the cases that bridges were constructed decades ago, grouts tending to bleed or swell were often used, and the grouting was not well done. As a result, ducts were not completely filled and voids remained. For the incompletely grouted zones, tendons were vulnerable and more severely corroded. To repair those bridges, effective and economic regrouting materials and method are requisite. Recently, a new repair grout has been developed in the authors’ group by mixing ion-exchange resin, a widely used polymer in water purification, into cement paste. Since IER can absorb chloride ions, IER mixed grout is promising to remove free chloride ions in the unfilled duct. In this study, the chloride ion diffusion properties of IER mixed grout was investigated experimentally. Tendons, which were preliminarily spread at the surface with chloride, were grouted using the IER mixed grout. After curing, the chloride amount in the grout was analyzed using potentiometric titration against silver nitrate. It was confirmed that IER effectively absorbed chloride ions from the surface of tendon, and thus is promising to alleviate the corrosion. Furthermore, to promote the practical use, other properties of the grout, such as strength, flowability and bleeding ratio, were test. It is found that as IER ratio increased strength and flowability tended to decrease but bleeding did not occur. When mix proportion and addition ratio of IER were adopted properly, those properties satisfied the acceptance criterias in design code.

scholarly journals A Study of Properties of Ion-Exchange Resin Mixed Grout: Chloride Diffusion, Strength and Rheology

◽  
2016 ◽  
Author(s):  
O. Sanada ◽  
◽  
H. Mutsuyoshi ◽  
A. Sumida ◽  
Y. Luan ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Chloride Diffusion

OPTIMIZATION OF DNA EXTRACTION METHODS FROM BIOLOGICAL MATERIALS OF HONEY BEES IN A DIFFERENT METAMOTPHOSIS PHASE

2016 ◽  
Vol 52 ◽  
pp. 171-176
Author(s):  
M. Palkina ◽  
O. Metlitska
Keyword(s):  
Ion Exchange ◽  
Dna Extraction ◽  
Honey Bees ◽  
Biological Material ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Extraction Methods ◽  
Chelex 100 ◽  
Drone Brood ◽  
Dna Extraction Methods

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]:  3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.

Chromatography of chlorinated biphenyls on an ion-exchange resin

1977 ◽  
Vol 49 (6) ◽  
pp. 764-766 ◽  
Author(s):  
Toshihiko. Hanai ◽  
Harold F. Walton
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin
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