Nonlinear Equilibrium and Axial Mixing Effects in Intraparticle Diffusion-Controlled Sorption by Ion Exchange Resin Beds. Experimental Study

1971 ◽  
Vol 10 (1) ◽  
pp. 65-70 ◽  
Author(s):  
C. J. Colwell ◽  
J. S. Dranoff
Keyword(s):  
Experimental Study ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Intraparticle Diffusion ◽  
Mixing Effects ◽  
Diffusion Controlled ◽  
Nonlinear Equilibrium

A Kinetic study of Cadmium(Ii) Adsorption on Lewatit Tp260 Resin

2003 ◽  
Vol 2003 (3) ◽  
pp. 144-146 ◽  
Author(s):  
Francisco José Alguacil
Keyword(s):  
Ion Exchange ◽  
Moving Boundary ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Langmuir Equation ◽  
Metal Adsorption ◽  
Particle Diffusion ◽  
Operating Variables ◽  
Concentration Time ◽  
Diffusion Controlled

Cadmium(II) adsorption from aqueous sulphate medium on Lewatit TP260 cationic (di-Na+) ion exchange resin was investigated. The influence of operating variables such as aqueous pH, metal concentration, time and temperature on the equilibrium parameters was studied. Cadmium(II) adsorption on the resin can described by the Langmuir equation, whereas cadmium(II) uptake is particle diffusion controlled. The moving boundary particle diffusion model only fits the initial metal adsorption on the resin.

scholarly journals Adsorption and Desorption Behavior of Ectoine Using Dowex® HCR-S Ion-Exchange Resin

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2068
Author(s):  
Yu-Chi Wu ◽  
Yu-Hong Wei ◽  
Ho-Shing Wu
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ph Value ◽  
Ion Exchange Resin ◽  
Intraparticle Diffusion ◽  
Second Order ◽  
Isotherm Models ◽  
Initial Concentration ◽  
Operation Conditions ◽  
Pseudo Second Order

Dowex® HCR-S ion-exchange resin was used to adsorb ectoine in a batch system under varying operation conditions in terms of contact time, temperature, pH value, initial concentration of ectoine, and type of salt. Six adsorption isotherm models (Langmuir, Freundlich, Temkin, Dubinin–Radushkevich, Sips, and Redlich–Peterson) and three kinetic models (pseudo-first-order, pseudo-second-order, and intraparticle diffusion) were used to investigate the ectoine adsorption mechanism of ion-exchange resin. According to the experimental results, the mechanism of ectoine adsorption using an ion exchanger includes the ion-exchange reaction and physisorption. Both the Langmuir and Freundlich models were found to have a high fitting. For the kinetic analysis, the pseudo-second-order and intraparticle diffusion models were suitable to describe the ectoine adsorption. Dowex® HCR-S resin has an average saturated adsorption capacity of 0.57 g/g and 93.6% of ectoine adsorption at 25~65 °C, with an initial concentration of 125 g/L. By changing the pH of the environment using NaOH solution, the adsorbed ectoine on the ion-exchange resin can be desorbed to 87.7%.

OPTIMIZATION OF DNA EXTRACTION METHODS FROM BIOLOGICAL MATERIALS OF HONEY BEES IN A DIFFERENT METAMOTPHOSIS PHASE

2016 ◽  
Vol 52 ◽  
pp. 171-176
Author(s):  
M. Palkina ◽  
O. Metlitska
Keyword(s):  
Ion Exchange ◽  
Dna Extraction ◽  
Honey Bees ◽  
Biological Material ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Extraction Methods ◽  
Chelex 100 ◽  
Drone Brood ◽  
Dna Extraction Methods

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]:  3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.

Chromatography of chlorinated biphenyls on an ion-exchange resin

1977 ◽  
Vol 49 (6) ◽  
pp. 764-766 ◽  
Author(s):  
Toshihiko. Hanai ◽  
Harold F. Walton
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin
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