scholarly journals The respiratory explosion activity of neutrophils in blood from patients with Graves' disease manifestation

2017 ◽  
Vol 63 (1) ◽  
pp. 4-8
Author(s):  
Sergey A. Dogadin ◽  
Margarita A. Dudina ◽  
Andrey A. Savchenko ◽  
Vladimir A. Mankovskiy ◽  
Ivan I. Gvozdev
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Area Under The Curve ◽  
Reactive Oxygen ◽  
Maximum Level ◽  
Burst Activity ◽  
Graves Disease ◽  
Oxygen Species ◽  
Respiratory Burst Activity ◽  
Disease Manifestation

  Objective. To study respiratory burst activity in neutrophilic granulocytes in the onset of Graves’ disease (GD). Material and methods. Twenty-six females aged 18—55 years (mean age, 40.7±13.2 years), with the diagnosis of Graves’ disease verified for the first time, were enrolled in this study. Spontaneous and zymosan-induced chemiluminescence (CL) was assessed using a CL3606 36-channel analyzer for 90 min. The following parameters of the chemiluminescence curve were determined: time to attain the maximum (Tmax), the maximum intensity (Imax), and the area under the curve (S). Results. Patients with GD and lucigenin-dependent CL of neutrophils exhibited decreased zymosan-induced CL and the increased Imax of luminol-dependent spontaneous and zymosan-induced CL of neutrophils. Association between the anti-TPO level in blood serum with Tmax (r=–0,70; р=0.036) and Imax of luminol-dependent induced CL (r=–0.72; р=0.030) was found. The maximum level of synthesis of secondary reactive oxygen species (ROS) was found to be elevated in patients with GP both at relative rest and upon antigen-induced respiratory burst. Conclusion. In patients with GD, respiratory burst activity in neutrophils increases due to synthesis of secondary reactive oxygen species. Upon the onset of GD, already at the stage of subclinical thyrotoxicosis, the association between the indices of respiratory burst in neutrophils and thyroid hormones is lost but there emerges a relationship between the CL response and anti-TPO concentration.

scholarly journals Thyroid Hormone-Induced Cytosol-to-Nuclear Translocation of Rat Liver Nrf2 Is Dependent on Kupffer Cell Functioning

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Luis A. Videla ◽  
Pamela Cornejo ◽  
Pamela Romanque ◽  
Catherine Santibáñez ◽  
Iván Castillo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Kupffer Cell ◽  
Respiratory Burst ◽  
Oxidase Inhibitor ◽  
Burst Activity ◽  
Oxygen Species ◽  
Respiratory Burst Activity ◽  
Nadph Oxidase Inhibitor ◽  
Nrf2 Activation

L-3,3′,5-triiodothyronine (T3) administration upregulates nuclear factor-E2-related factor 2 (Nrf2) in rat liver, which is redox-sensitive transcription factor mediating cytoprotection. In this work, we studied the role of Kupffer cell respiratory burst activity, a process related to reactive oxygen species generation and liver homeostasis, in Nrf2 activation using the macrophage inactivator gadolinium chloride (GdCl3; 10 mg/kg i.v. 72 h before T3[0.1 mg/kg i.p.]) or NADPH oxidase inhibitor apocynin (1.5 mmol/L added to the drinking water for 7 days before T3), and determinations were performed 2 h after T3. T3increased nuclear/cytosolic Nrf2 content ratio and levels of heme oxygenase 1 (HO-1), catalytic subunit of glutamate cysteine ligase, and thioredoxin (Western blot) over control values, proteins whose gene transcription is induced by Nrf2. These changes were suppressed by GdCl3treatment prior to T3, an agent-eliciting Kupffer-cell depletion, inhibition of colloidal carbon phagocytosis, and the associated respiratory burst activity, with enhancement in nuclear inhibitor of Nrf2 kelch-like ECH-associated protein 1 (Keap1)/Nrf2 content ratios suggesting Nrf2 degradation. Under these conditions, T3-induced tumor necrosis factor-α(TNF-α) response was eliminated by previous GdCl3administration. Similar to GdCl3, apocynin given before T3significantly reduced liver Nrf2 activation and HO-1 expression, a NADPH oxidase inhibitor eliciting abolishment of colloidal carbon-induced respiratory burst activity without altering carbon phagocytosis. It is concluded that Kupffer cell functioning is essential for upregulation of liver Nrf2-signaling pathway by T3. This contention is supported by suppression of the respiratory burst activity of Kupffer cells and the associated reactive oxygen species production by GdCl3or apocynin given prior to T3, thus hindering Nrf2 activation.

scholarly journals The Arabidopsis RboHB Encoded by At1g09090 Is Important for Resistance against Nematodes

2020 ◽  
Vol 21 (15) ◽  
pp. 5556 ◽  
Author(s):  
Abdalmenem I. M. Hawamda ◽  
Adil Zahoor ◽  
Amjad Abbas ◽  
Muhammad Amjad Ali ◽  
Holger Bohlmann
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Reactive Oxygen ◽  
Heterodera Schachtii ◽  
Oxygen Species ◽  
Cyst Nematodes ◽  
Feeding Site ◽  
Plant Life ◽  
Respiratory Burst Oxidase ◽  
Respiratory Burst Oxidase Homologue

Reactive oxygen species are a byproduct of aerobic metabolic processes but are also produced by plants in defense against pathogens. In addition, they can function as signaling molecules that control various aspects of plant life, ranging from developmental processes to responses to abiotic and biotic stimuli. In plants, reactive oxygen species can be produced by respiratory burst oxidase homologues. Arabidopsis contains 10 genes for respiratory burst oxidase homologues that are involved in different aspects of plant life. Plant pathogenic cyst nematodes such as Heterodera schachtii induce a syncytium in the roots of host plants that becomes a feeding site which supplies nutrients throughout the life of the nematode. In line with this function, the transcriptome of the syncytium shows drastic changes. One of the genes that is most strongly downregulated in syncytia codes for respiratory burst oxidase homologue B. This gene is root-specific and we confirm here the downregulation in nematode feeding sites with a promoter::GUS (β-glucuronidase) line. Overexpression of this gene resulted in enhanced resistance against nematodes but also against leaf-infecting pathogens. Thus, respiratory burst oxidase homologue B has a role in resistance. The function of this gene is in contrast to respiratory burst oxidase homologues D and F, which have been found to be needed for full susceptibility of Arabidopsis to H. schachtii. However, our bioinformatic analysis did not find differences between these proteins that could account for the opposed function in the interaction with nematodes.

Effects of raspberry fruit extracts and ellagic acid on respiratory burst in murine macrophages

2014 ◽  
Vol 5 (6) ◽  
pp. 1167-1174 ◽  
Author(s):  
Lina Raudone ◽  
Ramune Bobinaite ◽  
Valdimaras Janulis ◽  
Pranas Viskelis ◽  
Sonata Trumbeckaite
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Ellagic Acid ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Murine Macrophages ◽  
Fruit Extracts ◽  
Species Production

The main finding of our study is that raspberry extracts and ellagic acid inhibit reactive oxygen species production in PMA stimulated macrophages.

scholarly journals Alteration of Neutrophil Reactive Oxygen Species Production by Extracts of Devil’s Claw (Harpagophytum)

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Mbaki Muzila ◽  
Kimmo Rumpunen ◽  
Helen Wright ◽  
Helen Roberts ◽  
Melissa Grant ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Dna Analysis ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Putative Hybrid ◽  
Oxygen Species ◽  
New Variety ◽  
Devil’S Claw ◽  
Anti Inflammatory

Harpagophytum, Devil’s Claw, is a genus of tuberiferous xerophytic plants native to southern Africa. Some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. The objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of variousHarpagophytumtaxa in suppressing respiratory burst in terms of reactive oxygen species produced by human neutrophils challenged with phorbol myristate acetate (PMA), opsonisedStaphylococcus aureus,andFusobacterium nucleatum.Harpagophytumplants were classified into different taxa according to morphology, and DNA analysis was used to confirm the classification. A putative new variety ofH. procumbensshowed the highest degree of antioxidative capacity. Using PMA, threeHarpagophytumtaxa showed anti-inflammatory effects with regard to the PBS control. A putative hybrid betweenH. procumbensandH. zeyheriin contrast showed proinflammatory effect on the response of neutrophils toF. nucleatumin comparison with treatment with vehicle control.Harpagophytumtaxa were biochemically very variable and the response in suppressing respiratory burst differed. Further studies with larger number of subjects are needed to corroborate anti-inflammatory effects of different taxa ofHarpagophytum.

Surfactant protein A modulates release of reactive oxygen species from alveolar macrophages

1994 ◽  
Vol 267 (6) ◽  
pp. L660-L666 ◽  
Author(s):  
S. Weissbach ◽  
A. Neuendank ◽  
M. Pettersson ◽  
T. Schaberg ◽  
U. Pison
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Protein A ◽  
Reactive Oxygen ◽  
Surfactant Protein ◽  
Oxygen Radical ◽  
Surfactant Protein A ◽  
Oxygen Species ◽  
Coated Surfaces

The production and release of reactive oxygen species (the respiratory burst) is a common metabolic pathway linked to several macrophage-related reactions. The most abundant surfactant protein A (SP-A) binds to alveolar macrophages (AM) through a specific surface receptor with high affinity. Because such binding might initiate or modulate the respiratory burst, we wanted to know whether and how SP-A affects the oxygen radical release from AM. To answer these questions, we measured the release of reactive oxygen species from rat AM under various in vitro conditions using enhanced chemiluminescence systems. We prepared SP-A from pulmonary surfactant isolated either from silica-treated rats or adult dogs. Resident AM were harvested from pathogen-free Wistar rats by lung lavage. Adhered and nonadhered AM were assessed on protein-free or protein-coated surfaces of 96-well microtiter plates. On protein-free surfaces, the sole addition of SP-A failed to induce measurable oxygen radical release from 2 x 10(5) adhered or nonadhered AM, while zymosan opsonized with SP-A induced a marked increase over control. On protein-coated surfaces, AM respond differently depending on the coated protein: on SP-A-coated surfaces, a dose-dependent enhancement of oxygen radical release with a mean effective concentration of approximately 1.15 micrograms/ml was found. No such enhancement was seen on plates coated with similar amounts of either human fibronectin or collagen, and the enhancement with serum albumin was not dose related. Our data demonstrate that SP-A only enhances oxygen radical release from AM if SP-A is fixed to zymosan or the surface of the reaction vial in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)

Arabidopsis thaliana constitutively active ROP11 interacts with the NADPH oxidase respiratory burst oxidase homologue F to regulate reactive oxygen species production in root hairs

2016 ◽  
Vol 43 (3) ◽  
pp. 221 ◽  
Author(s):  
Min Yan ◽  
Wen Jing ◽  
Ni Xu ◽  
Like Shen ◽  
Qun Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Respiratory Burst ◽  
Root Hairs ◽  
Reactive Oxygen ◽  
Ros Production ◽  
Oxygen Species ◽  
Wild Type ◽  
Respiratory Burst Oxidase ◽  
Constitutively Active

Reactive oxygen species (ROS) play a key signalling role in cells. Plant NADPH oxidases, also known as respiratory burst oxidase homologues (Rbohs), are well characterised ROS-generating systems. In this study, we found that the constitutively active small guanosine triphosphatase (GTPase) ROP11 (CA-ROP11) interacted with RbohF by using a yeast two-hybrid analysis, a pull-down assay and an in vivo bimolecular fluorescence complementation assay. The mutation of amino acid L336 or L337 in RbohF abolished its interaction with CA-ROP11. Coexpression of CA-ROP11 and wild-type RbohF in Nicotiana benthamiana Domin enhanced ROS production compared with coexpression of CA-ROP11 and mutant RbohF or of dominant negative ROP11 and wild-type RbohF. Moreover, CA-ROP11 overexpression resulted in ROS accumulation and a swollen root hair phenotype in Arabidopsis thaliana (L.) Heynh. The deletion of RbohF partially reduced the increase in ROS in Arabidopsis plants overexpressing CA-ROP11. These results suggest that Arabidopsis ROP11 modulates ROS production by interacting with RbohF in root hairs.

Detection of the Production of Reactive Oxygen Species by Neutrophils in Whole Blood: Modulation by Adamantanes and Triggering by Fe3+-ions

1999 ◽  
Vol 54 (7-8) ◽  
pp. 562-568 ◽  
Author(s):  
Harald Schempp ◽  
Evelyn Albrecht-Goepfert ◽  
Erich F. Elstner
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Whole Blood ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Morbus Parkinson ◽  
Oxygen Species ◽  
Edta Complex ◽  
Activated Neutrophils ◽  
Radical Type

Abstract Using indicators for the production of reactive oxygen species (ROS) such as the a) OH- radical type (α-keto-γ-methiolbutyric acid. KMB) or b) hypochlorous acid (1-amino-cyclo- propvl-1-carboxylic acid, ACC) neutrophil activities can be both quantified and differentiated in whole blood via ethene production, Ethene is trapped in the head space of blood samples incubated in the presence of zymosan and the respective indicators, KMB or ACC. This procedure allows the detection of effects of aminoadamantanes (AAD) such as amantadine or memantine, compounds frequently used for the treatment of Morbus Parkinson and Morbus Alzheimer. In this report we describe the detection of OH-type oxidants produced by isolated activated neutrophils and whole blood. Immunomodulatory activities of AAD are deduced from the following observations: AAD-stimulated ethene formation from (KMB) as an indicator for production of OH′-type reactive oxygen species by zymosan-stimulated neutrophils (“respiratory burst”) is detectable with isolated neutrophils. In whole blood, how- ever, this reaction is only measurable in the presence of Fe-EDTA-complex. Stimulating effects of AAD are observed within a concentration range between 10-8 and 10-4 m with a maximum at 1 μΜ. Ethene release from (ACC) as indicator for the myeloperoxidase reaction after degranulation is not stimulated by AAD but inhibited at concentrations higher than 100 μΜ. The presented results suggest that submicromolar concentrations of AAD only stimulate the respiratory burst and apparently not degranulation of zymosan-prestimulated poly- morphonuclear neutrophils (PMN).

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