scholarly journals Fructosamine measurements in the early diagnosis of disordered carbohydrate metabolism

1994 ◽  
Vol 40 (6) ◽  
pp. 17-18
Author(s):  
E. G. Gasparyan ◽  
S. A. Nersesyan ◽  
Ye. L. Volkova ◽  
Z. V. Kryuchkova ◽  
S. Nassur ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Early Diagnosis ◽  
Glucose Tolerance ◽  
Carbohydrate Metabolism ◽  
Normal Subjects ◽  
Empty Stomach ◽  
Dependent Diabetes Mellitus ◽  
Serum Fructosamine ◽  
Insulin Dependent ◽  
First Time

Blood serum fructosamine level were measured on an empty stomach in 97 normal subjects and subjects with various disorders of carbohydrate metabolism (the so-called prediabetes and changed glucose tolerance) in order to elucidate the significance of this factor as a marker of such disorders. Fructosamine concentrations were for the first time measured in children whose parents suffered from insulin-dependent or noninsulin-dependent diabetes mellitus. The content of fructosamine on an empty stomach was found to depend on the degree of carbohydrate metabolism disorders, increasing with the progress of these disorders. The findings permit a conclusion on the possibility of using fructosamine as a marker for the diagnosis of prediabetes in subjects with its levels of 2.5 to 2.99 mmol/1 and of disordered glucose tolerance in those with its levels over 3 mmol/1 and glucose level on an empty stomach no more than 6 mmol/l.

scholarly journals Minidiab test to assess the function of pancreatic beta-cells in the children of diabetics

1994 ◽  
Vol 40 (6) ◽  
pp. 7-10
Author(s):  
E. G. Gasparyan ◽  
S. A. Nersesyan ◽  
Ye. A. Volkova ◽  
Z. V. Kryuchkova ◽  
L. I. Velikanova
Keyword(s):  
Diabetes Mellitus ◽  
Glucose Tolerance ◽  
Carbohydrate Metabolism ◽  
Pancreatic Beta Cells ◽  
Test Group ◽  
Normal Subjects ◽  
Dependent Diabetes Mellitus ◽  
Healthy Controls ◽  
C Peptide ◽  
Insulin Dependent

The levels of glucose, insulin, and C-peptide in the blood scrum were measured in 38 subjects with normal and impaired glucose tolerance whose parents suffered from insulin-dependent and noninsulin-dependent diabetes mellitus (IDDM and NID- DM, respectively) and in 12 normal subjects without hereditary aggravation for diabetes mellitus in order to specify the pecualiaritics of development of diabetes mellitus of various types. Reliably increased levels of glucose, insulin, and C-peptide on an empty stomach and absence of adequate secretion of insulin and C-peptide in response to stimulation with 5 mg of minidiab, expressed by a later and less manifest release of insulin and C-peptide, were observed in the test group, in contrast to healthy controls. The detected changes augment with the progress of carbohydrate metabolism disorders, being more marked in the subjects whose parents suffered from IDDM. The findings permit a conclusion that function of the insular system is changed during early disorders of carbohydrate metabolism in subjects whose parents suffered from both forms of diabetes mellitus. Minidiab test is recommended to specify the function of the pancreatic insular system.

Response of plasma somatostatin-like immunoreactivity (SLI) to a 75 g oral glucose tolerance test in normal subjects and patients with impaired glucose tolerance

1983 ◽  
Vol 104 (4) ◽  
pp. 468-474 ◽  
Author(s):  
Mitsuyasu Itoh ◽  
Yoshifumi Hirooka ◽  
Noriyuki Nihei
Keyword(s):  
Diabetes Mellitus ◽  
Glucose Tolerance ◽  
Human Peripheral Blood ◽  
Normal Subjects ◽  
Insulin Dependent Diabetes Mellitus ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Oral Glucose ◽  
Insulin Dependent ◽  
Somatostatin 14

Abstract. To study the role of somatostatin in the pathophysiology of glucose intolerance in man, plasma somatostatin-like immunoreactivity (SLI) was measured in 8 normal subjects, 6 patients with insulin dependent diabetes mellitus (IDDM), 13 with non-insulin dependent diabetes mellitus (NIDDM), and 9 with hyperthyroidism, by extraction of plasma SLI and radioimmunoassay. The extraction method gave a recovery rate for synthetic somatostatin-14 and somatostatin-28 of 72 ± 6 and 55 ± 7%, respectively. No SLI corresponding to somatostatin-28 in human peripheral blood was observed. Incubation of somatostatin-28 in plasma gave a rapid decrease of immunoreactivity, and no conversion to somatostatin-14 was observed. It is speculated that SLI extracted with acid-acetone mainly represents a molecular weight similar to somatostatin-14. After oral administration of glucose (75 g), a clear and sustained rise in plasma SLI was seen in normal subjects from an initial value (± sem) of 29.9 ± 5.4 pg/ml to a peak value, at 60 min of 93.4 ± 15.5 pg/ml. The increase of plasma SLI after 75 g glucose was also observed in IDDM and NIDDM. The peak level of SLI was significantly less than that for normal subjects. The extraction of plasma SLI with acetic acid and acetone gave reproducible results and showed a fluctuation of SLI with glucose concentration.

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