scholarly journals Intrathyroid stable iodine level and thyroid tumors

1996 ◽  
Vol 42 (3) ◽  
pp. 6-8
Author(s):  
I. O. Tomashevsky ◽  
Ye. A. Troshina ◽  
G. A. Gerasimov ◽  
M. E. Bronstein ◽  
S. Yu. Serpukhovitin ◽  
...  
Keyword(s):  
Clinical Studies ◽  
Thyroid Nodules ◽  
Iodine Concentration ◽  
Thyroid Tumors ◽  
X Ray ◽  
Fluorescent Analysis ◽  
Iodine Level ◽  
The Usa ◽  
Stable Iodine

Intrathyroid iodine level was measured in 126 patients with different thyroid tumours in Russia and in 37 ones in the USA by X-ray fluorescent analysis in vitro. A decrease of intrathyroid iodine concentration was found to be associated with a stepwise loss of differentiation of thyroid tumours. In colloid goitre tissue from Russia, the intrathyroid level of iodine was increased. It was markedly increased in microfollicular adenomas and colloid goitre tissue from American patients, which fact may reflect a higher iodine supply in the USA. X-Ray fluorescent analysis together with clinical studies may be used in preoperative examinations of patients with thyroid nodules.

scholarly journals Diagnostic possibilities for autoimmune thyroiditis using non-invasive determination of intrathyroid iodine concentration in children

2019 ◽  
Vol 45 (1) ◽  
pp. 21-23
Author(s):  
I. A. Vasilevskaya ◽  
I. O. Tomashevsky
Keyword(s):  
Enzyme Immunoassay ◽  
Case History ◽  
Autoimmune Thyroiditis ◽  
Microsomal Fraction ◽  
Iodine Concentration ◽  
Threshold Level ◽  
X Ray ◽  
Non Invasive ◽  
Stable Iodine

Sixty-seven children (55 girls and 12 boys) aged 5-16 years were examined. Autoimmune thyroiditis (AT) was diagnosed in 28 and diffuse nontoxic goiter (DNG) in 39. The diseases were diagnosed on the basis of case history, results of examination and palpation of the thyroid, ultrasonic findings, presence of antibodies to thyroglobulin (in AT) or their absence (in DNG), and the microsomal fraction detected by enzyme immunoassay using Boehringer Mannheim kits. AT diagnosis was confirmed cytomorphologically in all patients. Intrathyroid stable iodine (ISI) was measured by a Russian noninvasive x-ray fluorescent analyzer. ISI concentration was notably decreased in children with autoimmune thyroiditis confirmed by cytomorphological methods: below the threshold level of the method in 46%) cases and 120±10 mcg/g in 54%). This confirms a high informative value of the proposed method in this disease. Measurements of ISI in children with enlarged thyroid helps differentiate the hypertrophic form of AT from DNG: ISI concentration under 200 mcg/g is characteristic of AT, while in DNG the concentration of ISI in Moscow children is 500±40 mcg/g (M±m).

scholarly journals The ability to diagnose autoimmune thyroiditis with an X-ray fluorescence method to determine the level of stable intrathyroid iodine

1999 ◽  
Vol 45 (3) ◽  
pp. 28-30
Author(s):  
I. O. Tomashevsky
Keyword(s):  
Autoimmune Thyroiditis ◽  
Laboratory Data ◽  
Reference Specimen ◽  
Thyroid Status ◽  
Normal Thyroid ◽  
X Ray ◽  
Fluorescent Analysis ◽  
Fluorescent Method ◽  
History Of ◽  
Stable Iodine

The concentration of intrathyroid stable iodine (ISI) is measured using a Russian commercial reference specimen for noninvasive x-ray fluorescent analysis in 74 women with autoimmune thyroiditis (verified cytomorphologically) aged 36-55 years, residents of Moscow, and in 36 women without a history of thyroid diseases, with normal thyroid status confirmed by clinical and laboratory data and /SI level of at least 200 pg/g, which is considered as the critical (control). Noninvasive x-ray fluorescent method for detecting ISI deficiency permits the recognition of autoimmune thyroiditis with sensitivity and accuracy of at least 9% but with low specificity, which recommends it for screening diagnosis.

The iodine concentration in benign and malignant thyroid nodules measured by X-ray fluorescence

1981 ◽  
Vol 54 (643) ◽  
pp. 626-629 ◽  
Author(s):  
T. G. Tadros ◽  
M. N. Maisey ◽  
S. C. Ng Tang Fui ◽  
P. C. Turner
Keyword(s):  
Thyroid Nodules ◽  
Iodine Concentration ◽  
X Ray

Sem Studies of Co-Cr-Mo Surgical Implant Alloy Corrosion Behavior

1982 ◽  
Vol 40 ◽  
pp. 520-521
Author(s):  
Ann Chidester Van Orden ◽  
John L. Chidester ◽  
Anna C. Fraker ◽  
Pei Sung
Keyword(s):  
Electron Microscopy ◽  
Corrosion Behavior ◽  
Anodic Polarization ◽  
Saline Solution ◽  
Saturated Calomel Electrode ◽  
Physiological Saline ◽  
X Ray ◽  
Physiological Saline Solution ◽  
Scanning Electron

The influence of small variations in the composition on the corrosion behavior of Co-Cr-Mo alloys has been studied using scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), and electrochemical measurements. SEM and EDX data were correlated with data from in vitro corrosion measurements involving repassivation and also potentiostatic anodic polarization measurements. Specimens studied included the four alloys shown in Table 1. Corrosion tests were conducted in Hanks' physiological saline solution which has a pH of 7.4 and was held at a temperature of 37°C. Specimens were mechanically polished to a surface finish with 0.05 µm A1203, then exposed to the solution and anodically polarized at a rate of 0.006 v/min. All voltages were measured vs. the saturated calomel electrode (s.c.e.).. Specimens had breakdown potentials near 0.47V vs. s.c.e.

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

Preparation of cultured astrocytes for x-ray microanalysis

1989 ◽  
Vol 47 ◽  
pp. 988-989
Author(s):  
N.K.R. Smith ◽  
K.E. Hunter ◽  
P. Mobley ◽  
L.P. Felpel
Keyword(s):  
Cultured Cells ◽  
Elemental Content ◽  
Elemental Distribution ◽  
Sprague Dawley ◽  
X Ray ◽  
Cultured Astrocytes ◽  
Freeze Dried ◽  
Ultimate Objective

Electron probe energy dispersive x-ray microanalysis (XRMA) offers a powerful tool for the determination of intracellular elemental content of biological tissue. However, preparation of the tissue specimen , particularly excitable central nervous system (CNS) tissue , for XRMA is rather difficult, as dissection of a sample from the intact organism frequently results in artefacts in elemental distribution. To circumvent the problems inherent in the in vivo preparation, we turned to an in vitro preparation of astrocytes grown in tissue culture. However, preparations of in vitro samples offer a new and unique set of problems. Generally, cultured cells, growing in monolayer, must be harvested by either mechanical or enzymatic procedures, resulting in variable degrees of damage to the cells and compromised intracel1ular elemental distribution. The ultimate objective is to process and analyze unperturbed cells. With the objective of sparing others from some of the same efforts, we are reporting the considerable difficulties we have encountered in attempting to prepare astrocytes for XRMA.Tissue cultures of astrocytes from newborn C57 mice or Sprague Dawley rats were prepared and cultured by standard techniques, usually in T25 flasks, except as noted differently on Cytodex beads or on gelatin. After different preparative procedures, all samples were frozen on brass pins in liquid propane, stored in liquid nitrogen, cryosectioned (0.1 μm), freeze dried, and microanalyzed as previously reported.

Time-Resolved Cryo-Electron Microscopy of Oscillating Microtubules

1990 ◽  
Vol 48 (1) ◽  
pp. 502-503
Author(s):  
Eva-Maria Mandelkow ◽  
Ron Milligan
Keyword(s):  
Electron Microscopy ◽  
Dynamic Instability ◽  
Entire Solution ◽  
Reaction Scheme ◽  
Time Resolved ◽  
X Ray ◽  
X Ray Scattering ◽  
A Chain ◽  
Ray Scattering

Microtubules form part of the cytoskeleton of eukaryotic cells. They are hollow libers of about 25 nm diameter made up of 13 protofilaments, each of which consists of a chain of heterodimers of α-and β-tubulin. Microtubules can be assembled in vitro at 37°C in the presence of GTP which is hydrolyzed during the reaction, and they are disassembled at 4°C. In contrast to most other polymers microtubules show the behavior of “dynamic instability”, i.e. they can switch between phases of growth and phases of shrinkage, even at an overall steady state [1]. In certain conditions an entire solution can be synchronized, leading to autonomous oscillations in the degree of assembly which can be observed by X-ray scattering (Fig. 1), light scattering, or electron microscopy [2-5]. In addition such solutions are capable of generating spontaneous spatial patterns [6].In an earlier study we have analyzed the structure of microtubules and their cold-induced disassembly by cryo-EM [7]. One result was that disassembly takes place by loss of protofilament fragments (tubulin oligomers) which fray apart at the microtubule ends. We also looked at microtubule oscillations by time-resolved X-ray scattering and proposed a reaction scheme [4] which involves a cyclic interconversion of tubulin, microtubules, and oligomers (Fig. 2). The present study was undertaken to answer two questions: (a) What is the nature of the oscillations as seen by time-resolved cryo-EM? (b) Do microtubules disassemble by fraying protofilament fragments during oscillations at 37°C?

Evaluation of Antibacterial and Antidiabetic Potential of Silver Nanoparticles using Eugenia Uniflora L. Seed Extract

2020 ◽  
Vol 13 (6) ◽  
pp. 5217-5225
Author(s):  
Guru Kumar Dugganaboyana ◽  
Chethankumar Mukunda ◽  
Suresh Darshini Inakanally
Keyword(s):  
Biomedical Applications ◽  
Pathogenic Bacteria ◽  
Seed Extract ◽  
Drug Formulation ◽  
Visible Spectroscopy ◽  
Plant Materials ◽  
X Ray ◽  
Eugenia Uniflora ◽  
Uv Visible

In recent years, green nanotechnology-based approaches using plant materials have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the seed extract of the Eugenia uniflora L. (E.uniflora). Characterization was done using UV-Visible spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV-Visible spectroscopy (at 466 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in seed of E.uniflora extract accountable for the reduction of Ag+ ion and the stabilization of AgNPs was investigated. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AgNPs were evaluated for their antibacterial and along with their antidiabetic potential. The results showed that AgNPs are extremely effective with potent antidiabetic potential at a very low concentration. It also exhibited potential antibacterial activity against the three tested human pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. It could be concluded that E. uniflora seed extract AgNPs can be used efficiently for in vitro evaluation of their antibacterial and antidiabetic effects with potent biomedical applications.

Sign in / Sign up

Export Citation Format

Share Document