scholarly journals Deletion of Nuclear Factor kappa B p50 Subunit Decreases Inflammatory Response and Mildly Protects Neurons from Transient Forebrain Ischemia-induced Damage

2016 ◽  
Vol 7 (4) ◽  
pp. 450 ◽  
Author(s):  
Taisia Rolova ◽  
Hiramani Dhungana ◽  
Paula Korhonen ◽  
Piia Valonen ◽  
Natalia Kolosowska ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Nuclear Factor Kappa B ◽  
Nuclear Factor ◽  
Forebrain Ischemia ◽  
Transient Forebrain Ischemia ◽  
Nuclear Factor Kappa

BaeR protein acts as an activator of nuclear factor-kappa B and Janus kinase 2 to induce inflammation in murine cell lines

2016 ◽  
Vol 62 (9) ◽  
pp. 753-761
Author(s):  
Seung-Jin Lee ◽  
Biruk Tesfaye Birhanu ◽  
Elias Gebru Awji ◽  
Myung Hee Kim ◽  
Ji-Yong Park ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Nuclear Factor Kappa B ◽  
Inflammatory Responses ◽  
Janus Kinase ◽  
Cytokine Gene Expression ◽  
Raw 264.7 Cells ◽  
Nuclear Factor ◽  
Janus Kinase 2 ◽  
Nuclear Factor Kappa ◽  
Cox 2

BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to induce an inflammatory response. BaeR protein was purified from Salmonella enterica Paratyphi A and subcloned into a pPosKJ expression vector. RAW 264.7 cells were treated with BaeR, and RNA was extracted by TRIzol reagent for RT-PCR. Cytokine gene expression was analyzed by using the comparative cycle threshold method, while western blotting and ELISA were used to assess protein expression. We confirmed that BaeR activates nuclear factor-kappa B (NF-κB), thereby inducing an inflammatory response and increases the production of interleukins (IL-)1β and IL-6. During this process, the Janus kinase 2 (JAK2)–STAT1 signaling pathway was activated, resulting in an increase in the release of interferons I and II. Additionally, COX-2 was activated and its expression increased with time. In conclusion, BaeR induced an inflammatory response through activation of NF-κB in addition to the MAPKs. Furthermore, activation of the JAK2–STAT1 pathway and COX-2 facilitated the cytokine binding activity, suggesting an additional role for BaeR in the modulation of the immune system of the host and the virulence activity of the pathogen.

scholarly journals Sambucus australis Modulates Inflammatory Response via Inhibition of Nuclear Factor Kappa B (NF-kB) in vitro

2019 ◽  
Vol 91 (1) ◽  
Author(s):  
NORMA V.Q. CARNEIRO ◽  
HUGO B.F. DA SILVA ◽  
RAIMON R. DA SILVA ◽  
TAMIRES C.B. CARNEIRO ◽  
RYAN S. COSTA ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Nuclear Factor Kappa B ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa
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