scholarly journals A Comparative Assessment of the Antibacterial Efficacy of Licorice Mouth-Rinse with Chlorhexidine on Salivary Streptococcus mutans

2021 ◽  
Vol 10 (15) ◽  
pp. 1042-1047
Author(s):  
Jayashree Pathi ◽  
Kumudini Panigrahi ◽  
Ipsa Mohapatra ◽  
Ratikanta Tripathy
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Tertiary Care ◽  
Licorice Root ◽  
Antimicrobial Efficacy ◽  
Randomised Control Trial ◽  
Root Extract ◽  
Care Hospital ◽  
Mouth Rinse ◽  
Salivary Ph

BACKGROUND Dental and periodontal diseases are common problems worldwide. Strong association exists between Streptococcus mutans and dental caries. Mouthwashes like chlorohexidine and extracts of medicinal plants like liquorice have antimicrobial properties. The objective of the study was to compare the antimicrobial efficacy of licorice mouth-rinse with chlorhexidine on salivary Streptococcus mutans. METHODS A randomised control trial was undertaken in the department of Public Health Dentistry, in a tertiary care hospital of Bhubaneswar, Odisha. Children of both sexes, aged 7 to 14 years, with high risk of caries and providing willingness were included. The products used were Aqueous and Ethanolic licorice root extract – 15 gm and 375 mg / 10 ml respectively, Chlorhexidine 0.12 %. MIC of the products against Streptococcus mutans was determined. The children were divided into three groups, fifteen in each. Each participant rinsed with 10 ml of the randomly allocated prepared suspension for 1 min. Five saliva samples were collected from each, one pre-rinse and four post-rinse 2 mins, 30 mins, 1 hour and 2 hours after the intervention. Streptococcus mutans colony count and salivary pH was used to study the efficacy of the mouthwashes. RESULTS The study revealed that ethanolic extract of licorice had better antimicrobial efficacy. The efficacy of antimicrobial action of licorice extract at 30 minutes of rinsing and rise in salivary pH by use of both the preparations of licorice was significant as compared to the chlorhexidine group. CONCLUSIONS The antimicrobial and cariostatic efficacy of licorice extracts which was evident in the present study suggests and recommends that licorice can be used as a preventive regimen in clinical practice for diseases of mouth cavity especially dental caries. KEY WORDS Dental Caries, Streptococcus mutans, Licorice, Chlorhexidine, Mouth Wash

scholarly journals Frequency of Associated Factors Causing Dental Caries in Patients Reporting at Tertiary Care Hospital

2019 ◽  
Vol 28 (03) ◽  
pp. 118-121
Author(s):  
Zain Abid Khan ◽  
◽  
Beenish Qureshi ◽  
Usman Anwer ◽  
Syeda Kinza Asghar ◽  
...  
Keyword(s):  
Dental Caries ◽  
Associated Factors ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital

In vitro Antimicrobial Activities of 1-Methoxyficifolinol, Licorisoflavan A, and 6,8-Diprenylgenistein against Streptococcus mutans

2014 ◽  
Vol 49 (1) ◽  
pp. 78-89 ◽  
Author(s):  
Sug-Joon Ahn ◽  
Soon-Nang Park ◽  
Young Ju Lee ◽  
Eun-Jung Cho ◽  
Yun Kyong Lim ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Antimicrobial Activities ◽  
Licorice Root ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Root Extract ◽  
Gingival Fibroblast ◽  
Biofilm Development ◽  
Time Kill

The objective of the study was to investigate the antimicrobial effects of purified single compounds from ethanol-extracted licorice root on Streptococcus mutans. The crude licorice root extract (CLE) was obtained from Glycyrrhiza uralensis, which was subjected to column chromatography to separate compounds. Purified compounds were identified by mass spectrometry and nuclear magnetic resonance. Antimicrobial activities of purified compounds from CLE were evaluated by determining the minimum inhibitory concentration and by performing time-kill kinetics. The inhibitory effects of the compounds on biofilm development were evaluated using crystal violet assay and confocal microscopy. Cell toxicity of substances to normal human gingival fibroblast (NHGF) cells was tested using a methyl thiazolyl tetrazolium assay. Chlorhexidine digluconate (CHX) was used in the control group. Three antimicrobial flavonoids, 1-methoxyficifolinol, licorisoflavan A, and 6,8-diprenylgenistein, were isolated from the CLE. We found that the three flavonoids and CHX had bactericidal effects on S. mutans UA159 at the concentration of ≥4 and ≥1 µg/ml, respectively. The purified compounds completely inhibited biofilm development of S. mutans UA159 at concentrations over 4 μg/ml, which was equivalent to 2 μg/ml of CHX. Confocal analysis showed that biofilms were sparsely scattered in the presence of over 4 μg/ml of the purified compounds. However, the three compounds purified from CLE showed less cytotoxic effects on NHGF cells than CHX at these biofilm-inhibitory concentrations. Our results suggest that purified flavonoids from CLE can be useful in developing oral hygiene products, such as gargling solutions and dentifrices for preventing dental caries.

scholarly journals PERBANDINGAN EFEKTIFITAS JUS LIDAH BUAYA DAN KLORHEXIDIN 0.06% TERHADAP JUMLAH KOLONI STREPTOCOCCUS MUTANS SALIVA ANAK DENGAN KARIES

2018 ◽  
Vol 5 (1) ◽  
pp. 54
Author(s):  
Shanty Chairani ◽  
Sri W Rais ◽  
Rani Purba ◽  
Amalia AH
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Aloe Vera ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Control Group ◽  
Mouth Rinse ◽  
Active Components ◽  
Control Group Design

Background: Aloe vera contains active components such as antrhaquinones,saponin, glucomannans, and acemannans that have been known for its antimicrobial properties. In vitro study showed that Aloe vera extract can inhibit the growth of Streptococcus mutans. Studies reported that children with caries have high level of salivary S. mutans which is the main pathogen in dental caries. The gold standard mouthrinse for dental caries is chlorhexidine gluconate, but it has several side effects. The objective of this study was to compare the effect between Aloe vera juice and 0.06% chlorhexidine mouth rinse on salivary S. mutans in children with caries.Methods: This experimental research with pretest and post-test control group design conducted on 30 children (6-12 years old) with number of carious teeth >4. Subjects divided into 2 groups: Aloe vera juice group and 0.06% chlorhexidine group, which instructed to rinse their mouth with 10 ml stated mouthwash, twice a day, during 7-day period. Unstimulated saliva was collected at baseline and after 7 days then inoculated on mitis salivarius bacitracin agar. S. mutans were counted and expressed in CFU/ml. Data were analyzed using paired t-test and independent t-test.Result: The result showed that a significant reduction in salivary S.mutanscounts was observed with Aloe vera juice mouth rinse and 0.06% chlorhexidine (p<0.05). The mean reduction of S. mutans counts was not statistically significantly between grup (p>0.05).Conclusion: These findings suggest that Aloe vera juice can reduce salivary S. mutans in children with caries so it can be considered as alternative mouthwash to prevent caries.

scholarly journals Association of Dental Caries with Body Mass Index in Patients Attending Dental OPD of Tertiary Care Hospital, Pakistan: A Cross-Sectional Study

2021 ◽  
pp. 23-28
Author(s):  
Mahwish Memon ◽  
Azizullah Muhammad Nawaz Qureshi ◽  
Aosaf Anwar Memon ◽  
Sheba Ramzan ◽  
Abdul Ghani Shaikh ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Dental Caries ◽  
Body Mass ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Health Sciences ◽  
Permanent Teeth ◽  
Care Hospital ◽  
Sectional Study ◽  
Cross Sectional

Background: Dental caries area public health problem. Its prevalence is about 60%  in the Pakistani population. Purpose: The purpose of this study was to correlate the association between decayed, missing, and filled teeth (DMFT) score and its body mass index in patients visiting the Dental Department of Liaquat University of Medical and Health Sciences, Jamshoro. Materials and Methods: This cross-sectional study was conducted from January 2021 to May 2021 at the Department of Operative Dentistry Liaquat University of Medical and Health Sciences. Patients with age 16 to 70 years with presence of atleast 15 to 18 permanent teeth were included in te study. Patients having primary teeth were set in exclusion criteria. For Dental caries status, decayed, missing, and filled teeth (DMFT) index was used. Height and weight were measured using digital scales. BMI was calculated according to the formula weight in kilograms/height in centimeters. Data were analyzed for descriptive statistics. One Way ANOVA test was used for association. The statistical significance level was evaluated at p<0.05 Results: We were able to obtain the data of a total of 203 patients of which 57% were female.The mean age was 36.7±13.8. ). The mean DMTF score was 5.7±3.7. In association of BMI with DMTF score, females BMI was statistically significant with DMTF score with p=0.039 and in overall was also significant p =0.002 Conclusion: DMFT score was higher in overweight and obese individuals and it was significant statistically. However, large community based studies are required to confirm the association.

The antimicrobial effects of deglycyrrhizinated licorice root extract on Streptococcus mutans UA159 in both planktonic and biofilm cultures

Anaerobe ◽  
2012 ◽  
Vol 18 (6) ◽  
pp. 590-596 ◽  
Author(s):  
Sug-Joon Ahn ◽  
Eun-Jung Cho ◽  
Hyoung-Jun Kim ◽  
Soon-Nang Park ◽  
Yun-Kyong Lim ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Licorice Root ◽  
Root Extract ◽  
Antimicrobial Effects ◽  
Licorice Root Extract

scholarly journals Evaluation of Antimicrobial Efficacy of Pudina (Menthaarvenis), Tulsi (Ocimumtenuiflorum) and Curry Leaves (Murrayakoenigii) on Streptococcus mutans by Modified Direct Contact Test- An In vitro Study

2020 ◽  
Author(s):  
Hima Patel ◽  
Adish Anand Saraf ◽  
TM Mangala ◽  
Rushikesh Mahaparale ◽  
Sneha Mali ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Direct Contact ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Contact Test ◽  
Curry Leaves ◽  
Direct Contact Test

Introduction: Dental caries is a pandemic disease caused by Streptococcus mutans. Numerous preventive strategies have been developed to prevent dental caries with modern medicines. Recently, there has been a shift from use of modern medicines to herbal ayurvedic preparations which are easily available and cause least possible side effects but have not been tested against S. mutans. Aim: To evaluate antimicrobial value of Pudina, Tulsi and Curry leaves against S. mutans by modified direct contact test. Materials and Methods: This in vitro study used finely powdered form of Pudina, Tulsi and Curry leaves extracts (Hakim Chi Chi Pharmacy, Surat, India) without any added preservatives. The test microorganism S. mutans was isolated from human saliva using mitis salivarius agar. The Minimum Inhibition Concentration (MIC) was determined using agar well diffusion for Group 1 (Tulsi), Group 2 (Pudina), Group 3 (Curry leaves) and Group 4 (Chlorhexidine). Using this MIC, modified Direct Contact Test was performed for specific evaluation of the antimicrobial efficacy of each product. Chlorhexidine (0.2%) was used as a positive control for the comparison of each product. The results of modified direct contact test were subjected to ANOVA Test and Tukey’s Test using SPSS software version 23.0 which showed that when compared against chlorhexidine, the antimicrobial efficacy against S. mutans in decreasing order was of curry leaves, tulsi and pudina and the result of the study was significant. Results: Curry leaves showed higher antimicrobial efficacy than tulsi and pudina when compared against chlorhexidine (control) and the results were statistically significant (p<0.05). Conclusion: Curry leaf extract shows promising antimicrobial property against S. mutans and can be recommended for caries control with further investigations.

scholarly journals A Comparative Study on the Effect of Spilanthes acmella and Chlorhexidine (0.2%) Mouth Rinses on Salivary Streptococcus mutans Count in Children

2020 ◽  
pp. 79-86
Author(s):  
Sajeela Ismail ◽  
Sajid Ahamed ◽  
Nisha Elizabeth John ◽  
Ameera Ahmed Ismail
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Healthy Children ◽  
Mouth Rinse ◽  
Potassium Tellurite ◽  
Beneficial Effects ◽  
Mouth Rinses ◽  
Colony Forming Units ◽  
Spilanthes Acmella ◽  
In The Beginning

In the wake of an increased interest in finding natural alternatives for chemical medicinal products, this study assesses and compares the effects and acceptance of Spilanthes acmella, a widely used plant in various traditional systems of medicine, with Chlorhexidine (0.2%) mouth rinse on the dental caries causing pathogen Streptococcus mutans colonizing the oral cavity of children. The study included 40 healthy children in the age group of 8-12 years based on inclusion and extrusion criteria. In the beginning of the study, non-stimulated whole salivary samples were tested for the baseline count of colony forming units (CFU) of Streptococcus mutans using Mitis Salivarius Agar supplemented with potassium tellurite. The same process was repeated 24 hours after 15 days of regular use of mouth rinses twice daily. Results showed that there was statistically highly significant decrease in CFU in the post rinse phase in both the groups. S. acmella showed greater acceptance by study subjects when compared with the Chlorhexidine mouth rinse. It can be concluded based on the results of this study that S. acmella has beneficial effects against S. mutans and can be a potential option for preventive measures against dental caries, although more studies are needed for its validation.

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